Registration Dossier
Registration Dossier
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EC number: 290-370-1 | CAS number: 90131-58-1 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Thuya occidentalis, Pinaceae.
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- december 2017 to january 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Thuya occidentalis, ext.
- EC Number:
- 290-370-1
- EC Name:
- Thuya occidentalis, ext.
- Cas Number:
- 90131-58-1
- Molecular formula:
- not relevant for a UVCB substance
- IUPAC Name:
- Essential oil of thuya occidentalis obtained from leaves by steam distillation
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- batch number 4802
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- water treatment plant "STEP de Lille" 33290 Blanquefort (France) - The inoculum was a fresh sample of activated sludge and was collected from the aeration tank of a sewage treatment plant receiving predominantly domestic sewage.
- Duration of test (contact time):
- 28 d
- Details on study design:
- The medium was prepared from concentrated stock solutions in ultrapure water as decribed in the guideline.
Reference substance
- Reference substance:
- other: sodium benzoate
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- average
- Value:
- 58.44
- Sampling time:
- 28 d
- Details on results:
- The test item “HUILE ESSENTIELLE DE THUYA OCCIDENTALIS / THUYA OCCIDENTALIS OIL” contains no nitrogen according to the elementary analysis (see Appendix 6: Study plan, Annex 1-2), the evaluation of biodegradation has to be based in the following expressed as ThODNH4.
The degradation for the 10-day window beginning was reached on Day 5, at the end of the 10-day window on Day 15 the mean degradation was 45.87%, therefore the D-day window failed.
The degradation rate of the test item “HUILE ESSENTIELLE DE THUYA OCCIDENTALIS /THUYA OCCIDENTALIS OIL” reached 58.44% (54.40 to 63.05), following the ThODNH4, after 28 days of incubation.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The degradation rate of the test item “HUILE ESSENTIELLE DE THUYA OCCIDENTALIS /THUYA OCCIDENTALIS OIL” reached 58.44% (54.40 to 63.05), following the ThODNH4, after 28 days of incubation.
According to the OECD guideline 301F, the test item is not considered to be readily biodegradable.
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