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Diss Factsheets
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EC number: 222-613-4 | CAS number: 3555-47-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22nd March - 19th April 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on sampling:
- Test substance was not analysed
- Vehicle:
- no
- Details on preparation and application of test substrate:
- AMENDMENT OF SOIL
- Type of organic substrate: Speyer 2.3 soil
- Other:
APPLICATION OF TEST SUBSTANCE TO SOIL
- Method: Since the test substance was received as a liquid, a carrier was not used. Approximately half of the soil batch was transferred to a 4-L jar. An appropriate amount of the test substance was dosed dropwise across the soil surface using a pipette. The remaining half of the soil batch was then added to the 4-L jar. The soil was mixed by rolling the capped jar for approximately 3 hours at approximately 15 rpm on a rolling mill. The fortified soil was transferred to the bowl of a food mixer and mechanically mixed for 10 minutes at low speed. During this mechanical mixing, the soil was amended with 5.0 g of lucerne meal (i.e. alfalfa). Also during this mechanical mixing a volume of purified reagent water was added to the soil in order to bring the moisture content to 45 ± 5% of the soil’s maximum water holding capacity. After dosing and mixing, each batch of soil was divided equally into three replicate test samples in 1-L glass bottle test vessels.
VEHICLE:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): N/a
- Concentration of vehicle in test medium (stock solution and final test solution): N/a
- Evaporation of vehicle before use:N/a - Test organisms (inoculum):
- soil
- Total exposure duration:
- 28 d
- Test temperature:
- 20 ± 2 °C
- Moisture:
- The soil moisture content was maintained at approximately 45% of the maximum water holding capacity throughout the test.
- Organic carbon content (% dry weight):
- 0.6
- Details on test conditions:
- TEST SYSTEM
- Testing facility: Smithers Viscient, 790 Main Street, Wareham, Massachusetts 02571-1037
- Test container (type, material, size): The 1-L glass bottle test vessels were covered with perforated parafilm to allow for air exchange while reducing water loss.
- Amount of soil: 333g
- No. of replicates per concentration: 3
- No. of replicates per control: 3
- No. of replicates per vehicle control: N/a
SOIL INCUBATION
- Method: bulk / series of individual subsamples
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographical reference of sampling site (latitude, longitude): Offenbach, Rheinland-Pfalz, Germany.
- History of site:Over the past 4 years, no pesticides or organic fertilizers have been applied to the collection site.
- Vegetation cover: Not reported
- Treatments with pesticides or fertilizers: Over the past 4 years, no pesticides or organic fertilizers have been applied to the collection site.
- Accidental contamination: Not reported
- Other:
- Depth of sampling: 0-20cm
- Soil texture: USDA
- % sand: 68
- % silt: 26
- % clay: 6
- Soil taxonomic classification: Not reported
- Soil classification system: Not reported
- pH (in water): 6.5
- Initial nitrate concentration for nitrogen transformation test (mg nitrate/kg dry weight): 84.0-87.0 (unknown whether dry weight or not)
- Maximum water holding capacity (in % dry weight): 26.4
- Cation exchange capacity (mmol/kg): 5.7meq/100 g
- Pretreatment of soil: N/a
- Storage (condition, duration): refrigerated (approximately 4 °C) and in the dark.
- Initial microbial biomass as % of total organic C: 2.2
DETAILS OF PREINCUBATION OF SOIL (if any): Before use, the soil was transferred to an environmental chamber set to maintain a temperature of 20 °C and allowed to equilibrate to incubation conditions for 2 to 28 days
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Nitrogen formation rate
VEHICLE CONTROL PERFORMED: N/a
RANGE-FINDING STUDY
- Test concentrations: N/a
- Results used to determine the conditions for the definitive study:N/a - Nominal and measured concentrations:
- Nominal: Untreated control, 1.2, 3.7, 11, 33, and 100 mg/kg dry weight soil
- Reference substance (positive control):
- no
- Duration:
- 28 d
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Duration:
- 28 d
- Dose descriptor:
- EC25
- Effect conc.:
- > 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Details on results:
- - Effect concentrations exceeding solubility of substance in test medium: Not reported
- Other: - Results with reference substance (positive control):
- No reference test reported
- Reported statistics and error estimates:
- The nitrate formation rates were input into CETIS (Ives, 2019). Inhibition between the untreated control and the treated soils were tested for significance using the ANOVA and Dunnett’s Multiple Comparison Test statistical programs. All statistical conclusions were made at the 95% level of certainty. The nitrate formation rates were analyzed using the linear interpolation (ICPIN) statistical program in CETIS to determine EC10, EC25, and EC50 values (the concentrations at which nitrate formation is affected by 10, 25, and 50% relative to the untreated control, respectively). The results were investigated for outliers using the Grubbs Extreme Value Test statistical program in CETIS.
- Validity criteria fulfilled:
- yes
- Remarks:
- Relative standard deviation in nitrate concentration and formation rate for the untreated control samples ranged from 0.3 to 3.5%, meeting the 15% validity criteria.
- Conclusions:
- No effects were observed in a 28-day exposure of the test substance to soil microorganisms, producing EC10, EC25 and EC50 values of >100 mg/kg.
Reference
Table 1 - Nitrate concentration and formation rate
Nominal concentration (mg/kg) |
Value type |
Day 0 Nitrate concentration (mg/kg) |
Day 0 Nitrate concentration (mg/kg) |
Nitrate formation rate (mg/kg/day |
Untreated control
|
Mean |
87.0 |
254 |
5.98 |
SD |
0.26 |
5.97 |
0.21 |
|
RSD |
0.30 |
2.35 |
3.49 |
|
1.2
|
Mean |
85.8 |
285 |
7.11 |
SD |
0.05 |
2.26 |
0.08 |
|
RSD |
0.05 |
0.79 |
1.14 |
|
3.7
|
Mean |
86.3 |
255 |
6.03 |
SD |
0.09 |
7.48 |
0.27 |
|
RSD |
0.11 |
2.93 |
4.45 |
|
11
|
Mean |
84.7 |
244 |
5.68 |
SD |
0.62 |
3.89 |
0.14 |
|
RSD |
0.74 |
1.60 |
2.42 |
|
33
|
Mean |
84.0 |
256 |
6.13 |
SD |
0.92 |
2.87 |
0.09 |
|
RSD |
1.10 |
1.12 |
1.45 |
|
100
|
Mean |
84.6 |
246 |
5.77 |
SD |
0.93 |
1.02 |
0.07 |
|
RSD |
1.10 |
0.41 |
1.17 |
Description of key information
28-day EC10: >100 mg/kg dw (highest concentration tested) in accordance with OECD 216, based on read-across within the Reconsile Siloxane Category.
Key value for chemical safety assessment
Additional information
No data are available with the registration substance. Data from the structural analogue, dodecamethylpentasiloxane (L5, CAS 141-63-9), are read across.
A 28-day toxicity to soil microorganisms study for effects of L5 on nitrate formation rate of soil microflora has been conducted in accordance with OECD TG 216 (Soil Microorganisms: Nitrogen Transformation Test) at concentrations up to 100 mg/kg soil dry weight. No effects on nitrate formation rate were observed.
A 28-day EC50 value of >100 mg/kg soil dw and an EC10 value of >100 mg/kg soil dw (highest concentration tested) have been determined for the effects of dodecamethylpentasiloxane (L5, CAS 141-63-9) on nitrate formation rate of soil microflora based on nominal concentrations.
Analytical verification of test substance concentrations during the earthworm toxicity test indicate that the test substance is relatively stable in the soil. The soil was prepared using a similar method in both the earthworm and microorganisms tests, therefore it is likely that the soil microorganisms were exposed to the test substance throughout the test.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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