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Diss Factsheets
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EC number: 422-030-7 | CAS number: 91538-82-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Feb 15 - 20, 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- Activated Sludge, Respiration Inhibition Test, adopted April 04, 1984
- Deviations:
- yes
- Remarks:
- Due to technical reaseons the temperature of 18-22 °C according to the study protocol was exceeded to 23 °C.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 88/302/EEC
- Deviations:
- yes
- Remarks:
- Due to technical reaseons the temperature of 18-22 °C according to the study protocol was exceeded to 23 °C.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Due to the low solubility of the test item in test water, adequate amounts of the test item were directly weighed into each test flask. One test solution with a final volume of 500 mL was tested per treatment in a glass flask (>500 mL). Samples were prepared in about 15-minute intervals.
The following test item concentrations have been used: 1000, 320, 100, 32, and 10 mg/L - Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Species: activated sludge, micro organisms from a domestic waste water treatment plant.
Origin: supplied by the sewage plant Darmstadt, Germany
Conditioning:
The sludge was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was re-suspended in tap water and again centrifuged. The latter pro¬cedure was repeated twice. An aliquot of the fmal sludge sus-pension was weighed, dried and the ratio of wet sludge (g) to its dry weight (g) determined. The washed sludge suspension was made up with tap water and 50 mL synthetic sewage feed (see 6.6) per litre was added and the sludge was kept at room temperature under continuous aeration for two days until use. Immediately before use, the dry weight of the activated sludge was determined, and diluted to 2 g/L with tap water. The pH of the activated sludge was 7.1. - Test type:
- other: aerobic
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20-23 °C
- pH:
- 7-8.5
- Dissolved oxygen:
- 7.3 mg/L
- Nominal and measured concentrations:
- 1000, 320, 100, 32, and 10 mg/L
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- EC50 = 13.2 mg/L (Acceptance range: 5-30 mg/L)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 3-hour EC20 and EC50 are clearly higher than 1000 mg/L under the present test conditions.
- Executive summary:
Purpose
The influence of the test item on the activity of activated sludge was evaluated by measuring the respiration rate under defined conditions.
Study design
The respiration rate (oxygen consumption) of an aerobic activated sludge fed with a standard amount of synthetic sewage was measured in the presence of various concentrations (10, 32, 100, 320 and 1000 mg/L) of the test item after an incubation period of 3 hours. This study was conducted according to Good Laboratory Practice (GLP) and the method applied followed the OECD Guideline for Testing of Chemicals, Section 2, No.209: "Activated Sludge, Respiration Inhibition Test", adopted April 04, 1984.
Results
In comparison to the inoculum controls the respiration rate of the activated sludge was slightly inhibited between 0 % and 5.9 % in a concentration range from 10 mg/L up to 1000 mg/L. Concentrations exceeding 1000 mg/L nominal were not tested.
Based on measured inhibition rates, the 3-hour EC 20 and EC 50 could not be quantified because up to the highest nominal test concentration of 1000 mg/L less than 20 % inhibition was noted after three hours incubation. Nevertheless, the 3-hour EC 20 and EC 50 are clearly higher than 1000 mg/L under the present test conditions.
The 3-hour EC 50 for the positive control 3,5 -dichlorophenol, which was tested in the same way as the test item, was found to be 13.2 mg/L and is within the range of 5 - 30 mg/L recommended by the test guidelines; thus, confirming suitability of the activated sludge.Conclusion
The 3 -hour EC20and EC50 of the test material are clearly higher than 1000 mg/L under the present test conditions.
Reference
Results and Discussion
Inhibition of Respiration Rate
In comparison to the inoculum controls the respiration rate of the activated sludge was slightly inhibited between 0%and 5.9 % in a concentration range from 10mg/Lup to 1000 mg/L. Concentrations exceeding 1000 mg/L nominal were not tested.
3-hour EC20/80and 3-hour EC50:
Based on measured inhibition rates, no 3-hour EC20/80and EC50could be quantified because up to the highest nominal test concentration of 1000 mg/L less than 10%inhibition was noted after three hours incubation. Nevertheless, the 3-hour EC20/80and EC50are clearly higher than 1000 mg/L under the present test conditions.
Description of key information
Based on measured inhibition rates, no 3-hour EC20/80 and EC50 could be quantified because up to the highest nominal test concentration of 1000 mg/L less than 10% inhibition was noted after three hours incubation. Nevertheless, the 3-hour EC20/80 and EC50
are clearly higher than 1000 mg/L under the present test conditions.
Key value for chemical safety assessment
Additional information
The influence of the test material on the growth and growth rate of the green algae species Desmodesmus subspicatus was tested. Therefore, a GLP compliant study according to OECD TG 201 was undertaken. The study design included one control and one test material group with three replicates, each containing 100 mL reconstituted water or test medium and about 10,000 cells/mL at the start of the experimental phase. The study was performed as a limit test in an open static test system. The algae were exposed to a filtrate of nominal 100 mg/L. The growth of the algae was calculated after 24, 48, and 72 hours exposure in the test medium. The analysis of the saturated aqueous preparation revealed that the water solubility of the test item in reconstituted water was < 0.0114 mg/L. An exact value could not be determined. The test material concentration in the aqueous medium could not be quantified since it was below the limit of quantification.
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Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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