Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 223-228-4 | CAS number: 3775-90-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Review by Japanese government and included in the SIDS dossier for 2-dimethylaminoethyl methacrylate as Klimisch 1.
Data source
Referenceopen allclose all
- Reference Type:
- review article or handbook
- Title:
- 2-Dimethylaminoethyl methacrylate SIDS Initial Assessment Report for SIAM 14
- Author:
- OECD SIDS
- Year:
- 2 002
- Bibliographic source:
- OECD SIDS Initial Assessment Report For SIAM 14, Paris, France, 26-28 March 2002
- Reference Type:
- secondary source
- Title:
- Unnamed
- Year:
- 1 989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- MADAME
- IUPAC Name:
- MADAME
- Reference substance name:
- 2-dimethylaminoethyl methacrylate
- EC Number:
- 220-688-8
- EC Name:
- 2-dimethylaminoethyl methacrylate
- Cas Number:
- 2867-47-2
- Molecular formula:
- C8H15NO2
- IUPAC Name:
- 2-(dimethylamino)ethyl methacrylate
- Test material form:
- other: liquid
- Details on test material:
- Purity <99.0%
Constituent 1
Constituent 2
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Summary does not include these details
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- Negative control, distilled water
Positive control: Cyclophosphamide , physiological saline (0.9% NaCl) - Details on exposure:
- Single oral (gavage) dose followed by sampling at 24hour, 48hour and 72 hours after dosing.
- Duration of treatment / exposure:
- Single oral (gavage) dose followed by sampling at 24hour, 48hour and 72 hours after dosing.
- Frequency of treatment:
- Single dose
- Post exposure period:
- Up to 72 hours
Doses / concentrations
- Remarks:
- Doses / Concentrations:
1000 mg/kg limit dose
Basis:
nominal in water
- No. of animals per sex per dose:
- 5
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- Cyclophosphamide, dose 40mg/kg bodyweight
Examinations
- Tissues and cell types examined:
- Polychromatic erythrocytes from the bone marrow in the femur.
- Details of tissue and slide preparation:
- Summary does not include these details
- Evaluation criteria:
- Cytotoxicty was assess by comparing the ratio of the PCE (polychromatic erythrocytes) the NCE (normochromatic erthrocytes follwing and staining. A decrease in the PCE/NCE ratio compared to the negative controls would be taken to indicate cytotoxicty and confirm that the test substnace or a metaboite have reached the bone marrow.
- Statistics:
- Summary does not include these details
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Vehicle controls validity:
- valid
- Negative controls validity:
- not applicable
- Positive controls validity:
- valid
Any other information on results incl. tables
Sampling time: 24 hours
Group | Dose mg/kg Bwt | PCE with Micronuclei | Micronuclei in 1000 PCE | PCE/NCE (mean) |
Solvent control | 0 | 0.06 | 0 - 2 | 1000/554 |
2 -dimethylaminoethyl methacrylate | 1000 | 0.03 | 0 - 2 | 1000/653 |
Cyclophosphamide | 40 | 0.75 | 1 - 13 | 1000/742 |
Sampling Time: 48 hours
Group | Dose mg/kg bw | PCE with Micronuclei | Micronuclei in 1000 PCE | PCE/NCE (mean) |
Solvent control | 0 | 0.04 | 0 - 2 | 1000/680 |
2 -dimethylaminoethyl methacrylate | 1000 | 0.04 | 0 - 1 | 1000/744 |
Sampling time: 72 hours
Group | Dose mg/kg bw | PCE with Micronuclei | Micronuclei in 1000 PCE | PCE/NCE (mean) |
Solvent control | 0 | 0.06 | 0 - 4 | 1000/594 |
2 -dimethylaminoethyl methacrylate | 1000 | 0.09 | 0 - 2 | 1000/506 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
2-dimethylaminoethyl methacrylate was not clastogenic in vivo in the mouse micronucleus test at a 1000 mg/kg limit dose which produced toxic effect in the mice in a preliminary study. There was no indication of cytotoxicity in the bone marrow. - Executive summary:
Type : Micronucleus assay
Species : mouse
Sex : male/female
Strain : NMRI
Route of admin. : gavage
Exposure period : one dose
Doses : 1000 mg/kg (maximum tolerated dose)
Result : negative
Method : OECD Guideline 474 "Genetic Toxicology: Micronucleus Test"
Year : 1989
GLP : yes
Test substance : as prescribed by 1.1 - 1.4
Remark : In comparison with the corresponding negative controls there was no
substantial enhancement in the frequency of the detected micronuclei at
any preparation interval after application of the test article. The mean values
of micronuclei observed after treatment with MADAME were in the same
range compared to the negative control groups. In the positive control group
a distinct increase of induced micronuclei frequency was observed.In
conclusion, the test article did not induce micronuclei as determined by the
micronucleus test in the bone marrow cells of the mouse.
Source : Roehm,
EUROPEAN COMMISSION - European Chemicals Bureau Ispra (VA)
Test condition : Group: 5 males and 5 females
Negative control: distilled water
Positive control: Cyclophosphamid in physiological serum (NaCl)
Dose: 40 mg/kg
Bone marrow preparation: 24, 48 and 72 hrs after application.
Analysis : 1000 PCE (Polychromatic Erythrocytes) per animal
By a preliminary test, 1000 mg/kg b.w. was estimated to be the maximum
tolerated dose. The animals expressed toxic reactions. After treatment with
the test article the ratio between PCEs and NCEs was not affected as
compared to the corresponding negative controls, thus indicating no
cytoyoxic effects.
Conclusion
2-dimethylaminoethyl methacrylate was not clastogenic in vivo in the mouse micronucleus test at a 1000 mg/kg limit dose.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.