Sodium 1-amino-9,10-dihydro-9,10-dioxo-4-[(2,2,4-trimethylcyclohexyl)amino]anthracene-2-sulphonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From April 06th to September 27th, 1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

part I, adopted 1984

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

September 19, 1984

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Test item was dissolved in the test medium at a concentration of 100 mg/100 ml (stock solution). Thereafter, series of sequential dilutions with the test medium were prepared to obtain the final test concentrations of the test article.

Test organisms (species):

Daphnia magna

Details on test organisms:

Animals were bred in the testing laboratory, under standardized conditions.
for the test, animals with an age of less than 24 hours were used.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Test temperature:

21.5 °C

pH:

Start: 8.4
End: 8.3

Dissolved oxygen:

Start: 8.6 mg/l
End: 9.0 mg/l

Nominal and measured concentrations:

Nominal 0, 1, 10 and 100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 ml beakers.
- Volume: beakers contain 20 ml of test medium.
- No. of organisms per vessel: 10 animals.
- No. of vessels per concentration: 2 replicates.
- No. of vessels per control: 2 replicates.

TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: bidistilled water.
- Test medium: according to the Directive EEC.
- CaCl2 x 2H2O: 294 mg/l
- MgSO4 x 7H2O: 123 mg/l
- NaHCO3: 65 mg/l
- KCl: 5.8 mg/l.

OTHER TEST CONDITIONS
- Adjustment of pH:pH of reconstituted water was adjusted to 7.9 ± 0.3.
- Photoperiod: 16 hours of illumination.
- Light intensity: 1000 Lux.

EFFECT PARAMETERS MEASURED
Test evaluations were done according to the Guideline No. 202 of the OECD by visual control of the mobility of the daphnids after 24 hours. Based on the data, an estimate of the EC0, 50 and 100 was given.

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No deaths occurred over the exposure period of 24 hours. EC0 (24h): 100 mg/l

Appearance of the test medium: blue solution.

Influence of test item on the immobilization of Daphnia magna, after 24 hours of exposure

Concentration (mg/l)	N. of daphnids	N. of responses	% of responses
Control	10	1	10
Control	10	0	0
Test item 1	10	0	0
Test item 1	10	0	0
Test item 10	10	0	0
Test item 10	10	0	0
Test item 100	10	0	0
Test item 100	10	0	0

Conclusions:

EC50 (24h) > 100 mg/l (nominal)

Executive summary:

The acute toxicity of the test substance to Daphnia magna was investigated over a period of 24 hours, following the OECD guideline 202, part I (1984). The nominal test concentrations applied were 0, 1, 10 and 100 mg/l; all tests were run in duplicate. No chemical analysis of the test substance concentration was conducted. No deaths occurred at the nominal concentration of 100 mg/l; 10 % of mortality was observed in one of the two blank controls. The EC0 was identified to be 100 mg/l. Test medium appeared to be blue solution.

Conclusion

EC50 (24h) > 100 mg/l (nominal)

Description of key information

EC50 (24h) > 100 mg/l (nominal)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

The acute toxicity of the test substance to Daphnia magna was investigated over a period of 24 hours, following the OECD guideline 202, part I (1984). No chemical analysis of the test substance concentration was conducted. No deaths occurred at the nominal concentration of 100 mg/l; 10 % of mortality was observed in one of the two blank controls. The EC0 was identified to be 100 mg/l. Test medium appeared to be blue solution.