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EC number: 279-506-0 | CAS number: 80584-92-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- EL
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: algae separated by centrifugation before analysis
- Vehicle:
- no
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: G. Brinkmann 1953/SAG86,81
- Source: Institute of Botany, Slovak Academy of Sciences, Bratislava, Slovak Republic
- Method of cultivation: inoculum culture prepared 3 days before test start. Conditions same as under test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 22 - 24 °C
- pH:
- 7.98 - 8.16
- Nominal and measured concentrations:
- Initial concentrations: 64, 128, 256, 512, 1024, 2048, 3000 mg/L (nominal)
Measured concentrations at start and end of the test: between -0.27% and +9.91% deviation of nominal - Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 mL volume in 150 mL flasks
- Type: closed with air-permeable stoppers
- Materia: glass or other chemically inert material
- Initial cells density: 4160 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes, according to OECD
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Sörensen buffer
- Intervals of water quality measurement: pH measured at beginning and end of test
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: stock solution adjusted with 1 N NaOH to pH 8.04
- Photoperiod: continuous illumination
- Light intensity and quality: 8470 - 8590 lux, cool white light, effective wavelength 400 - 700 nm - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 145 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI: 115-181 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3 285 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI: 2619-4119 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 452 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CI: 247-825 mg/L
- Results with reference substance (positive control):
- - 72 h-ECr10: 0.32 mg/L (mean of 3 determinations)
- 72 h-ECr50: 0.81 mg/L (mean of 3 determinations) - Reported statistics and error estimates:
- y
- Validity criteria fulfilled:
- yes
- Executive summary:
A growth inhibition test of TC to the algae Desmodesmus subspicatus was performed according to OECD 201. After 72 h of exposure, the ECr10 was determined to be 145 mg/L and the ECr50 to be 3285 mg/L.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test procedure in accordance with national standard methods (DIN 38412, part 9); peer-reviewed data from reliable database; legally binding classification (KBwS, WGK 1)
- Qualifier:
- according to guideline
- Guideline:
- other: German Industrial Standard DIN 38412 part 9
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Vehicle:
- not specified
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- pH:
- tested in neutralised and non-neutralised test medium in parallel
- Details on test conditions:
- - algae test apparatus with quasi-continuous determination of optical density
- counting of cell numbers
- photometric determination of turbidity at pre-determined times
- test solutions tested non-neutralized and neutralized - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 512 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- neutralised
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 26 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- neutralised
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 216 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- non-neutralised
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 7.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- non-neutralised
- Basis for effect:
- growth rate
- Validity criteria fulfilled:
- yes
Referenceopen allclose all
Growth data:
Nominal concentration |
Inhibition of growth rate |
Inhibition of biomass |
64 |
1.00 |
4.4 |
128 |
6.71 |
21.2 |
256 |
18.11 |
42.3 |
512 |
25.66 |
60.8 |
1024 |
34.24 |
72.3 |
2048 |
43.65 |
76.9 |
3000 |
50.03 |
83.9 |
Description of key information
The compound consists of a triazine part and an amine part. Under environmental conditions it is present in its dissociated form. Therefore, separate assessments were conducted for the triazine and the amine part, respectively.
CAS 80584-91-4: There is a high probability that the compound is not acutely harmful to algae.
CAS 102-71-6: There is a high probability that the compound is not acutely harmful to algae.
Key value for chemical safety assessment
Additional information
The compound consists of a triazine part and an amine part. Under environmental conditions it is present in its dissociated form. Therefore, separate assessments were conducted for the triazine and the amine part, respectively.
CAS 80584-91-4
Effects to alga was assessed in a 72-h growth inhibition test with Desmodesmus subspicatus in accordance to OECD guideline 201. The test was conducted with concentrations of 64, 128, 256, 512, 1024, 2048, 3000 mg/L.
ErC10 = 145 mg/L, ErC50 = 3285 mg/L, Ey50 = 452 mg/L
CAS 102-71-6
The assessment of the algal toxicity is based on effect data taken from the “Commission for the Evaluation of Substances Hazardous to Waters” (KBwS) data sheet no. 201, which is part of the German Federal Water Act (Article 19g). Based on the collected data a substance is classified according to its water-hazardous properties (WGK 1 to 3). Triethanolamine was classified as WGK 1, which indicates a low hazard to waters. As the list with the classified substances was published in the Bundesanzeiger (Ref. 4), the classification is legally binding for the industry.
The data sheet lists three sources for effect data on algae (see table below) of which only one was performed according to a national standard method (Amann & Steinhäuser, Ref. 1) while the other two are of questionable reliability due to exposure period or documentation (Bringmann & Kühn, Ref. 2; Verschueren, Ref. 8).
Species |
Not neutralised |
Neutralised |
Remark |
Ref. |
||
EC10 (mg/L) |
EC50 (mg/L) |
EC10 (mg/L) |
EC50 (mg/L) |
|||
Desmodesmus subspicatus |
7.9 |
216 |
26 |
512 |
DIN 38412, part 9; exposure: 72 h; based on growth rate |
1 |
Scenedesmus quadricauda |
EC3: 1.8 |
- |
EC3: 715 |
- |
Confusion between results for neutralised and not neutralised test results; here displayed correctly; exposure: 8 d (non-standard duration); no guideline followed |
2 |
Scenedesmus |
EC0: 100 |
- |
- |
- |
No information regarding exposure duration and conditions; test species not clearly identified; based on growth rate |
8 |
Additional information |
||||||
Phaeodactylum tricornutum |
> 10; NOEC: < 28 |
204 |
- |
- |
Seawater species, ISO 10253; exposure: 72 h with light/dark cycle 16/8 h |
7 |
Skeletonema costatum |
- |
>107 -<260 |
- |
- |
Seawater species, ISO 10253; exposure: 72-h, constant light |
9, 10 |
Desmodesmus subspicatus |
- |
- |
110 |
750 |
DIN 38412, part 9; exposure: 48 h |
6 |
Colpoda |
EC0: 160 |
- |
- |
- |
No information regarding exposure duration and conditions; test species not clearly identified; based on growth rate |
8 |
The data from Amann & Steinhäuser (Ref. 1) were generated within the research project “Assessment of Water-Hazardous Substances” which served the scientific support and administration of a “Commission” in their work of the German Federal Ministry for the Environment, Nature Conservation and Nuclear Safety.
The algal toxicity tests were performed according to DIN 38412, part 9 (draft). Tests were performed in neutral medium unless noted otherwise. In case of triethanolamine, the test was performed with neutralised and not neutralised test medium in parallel. The test species wasScenedesmus subspicatus(new name:Desmodesmus subspicatus). The EC10 was determined being the relevant factor for the WGK classification. The effect data were evaluated based on growth rate in accordance with the information requirements of REACH.
The effect values show a higher toxicity using non-neutralised test medium; therefore, part of the observed toxicity is caused by the pH change due to the substance. For the risk assessment the effect data for the neutralised test medium should be used.
Detailed data on the study itself, e.g. biomass growth rate as well as growth curves are not available. Nevertheless the test results were performed according to national standards and peer-reviewed by the KBwS during the performance of the research project. Therefore, the data can be regarded as reliable with restrictions (RL 2) and should be used as key study for the risk assessment.
The result by Amann & Steinhäuser (1) is supported by the published effect values for a seawater algaePhaeodactylum tricornutumby Libralato et al. (2010) and the information on the seawater algae Skeletonema costatum published by Eide-Haugmo et al. (2009/2012). Other data which are available for triethanolamine do not contradict the selected key values, but should not be used for risk assessment purposes due to insufficient exposure duration and documentation.
The study performed by Amann & Steinhäuser was identified as key study. A 72-h ErC50 of 216 mg/l for non-neutralized samples and a 72-h ErC50 of 512 mg/l for a neutralized sample were determined onDesmodesmus subspicatusaccording to German Industrial Standard DIN 38412, part 9 (draft). The corresponding ErC10 values are 7.9 (non-neutr.) and 26 mg/L (neutr.). This result is supported by published data on a seawater algae (Phaeodactylum tricornutum; Libralato et al., 2010), which gave similar effect values in non-neutralised test solutions: EC50 = 204 mg/L; EC10 > 10 mg/L; NOEC < 28 mg/L. In addition, the seawater algae Skeletonema costatum (Eide-Haugmo et al., 2009/2012) also supports this level of sensitivity, although the EC50 could only be determined as a range of > 107 and < 260 mg/L.
Based on the presented data, it can be concluded that triethanolamine is acutely not harmful to algae.
References
1) Amann W. & Steinhäuser KG (1986, 1988). 1. und 2. zusammenfassender Zwischenbericht zum F+E Vorhaben „Bewertung wassergefährdender Stoffe“ Teil I (FV-Nr. 82-102 05 308); Bayerisches Landesamt für Wasserwirtschaft, München [Interim Report, Evaluation of substances hazardous to water].
2) Bringmann G. & Kühn R. (1977). Z Wasser Abwasser Forsch 10, 87-98.
3) DIN 38412, part 9 (draft). German standard methods for the examination of water, waste water and sludge; bio-assays (group L); determination of the inhibitory effect of water constituents on the growth ofScenedesmusgreen algae (L 9).
4) Federal Ministry of Justice (2005). Bundesanzeiger No. 142 a.
5) ISO, Standard 10253 (2006). Water Quality—Marine Algal Growth Inhibition Test withSkeletonema costatumandPhaeodactylum tricornutum.
6) Kühn R and Pattard M (1990). Results of the harmful effects of water pollutants to green algae (Scenedesmus subspicatus) in the cell multiplication inhibition test. Water Research 24(1), 31-38.
7) Libralato G., Volphi Ghirardini A., Avezzù F. (2010). Seawater ecotoxicity of monoethanolamine, diethanolamine and triethanolamine. J Hazardous Materials 176, 535-539.
8) Verschueren K. (1983). Handbook of environmental data on organic chemicals, 2nd edition. Van Nostrand Reinhold Company, New York.
9) Eide-Haugmo I, Brakstad OG, Hoff KA, Sorheim KR, Falck da Silva E, Svendsen HF (2009). Environmental impact of amines. Energy Procedia 1: 1297-1304.
10) Eide-Haugmo I, Brakstad OG, Hoff KA, Falck da Silva E, Svendsen HF (2012).Marine biodegradability and ecotoxicity of solvents for CO2-capture of natural gas.International Journal of Greenhouse Gas Control 9: 184-192.
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