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EC number: 228-779-4 | CAS number: 6358-57-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- adopted April 4, 1984
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Acid Red 111
- IUPAC Name:
- Acid Red 111
Constituent 1
Test solutions
- Details on test solutions:
- The test aricle was dissolved in bidistilled water at a concentration of 500 mg/l.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source: ARA, Sissach/Switzerland
- Pre-treatment: sludge was coarsely sieved, centrifuged, the supernatant liquid phase decanted, the solid material resuspended in tap water and again centrifuged. This procedure was repeated a further two times. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge (ml) to its dry weight (g) detrmined.
- Preparation: a volume of washed sludge suspension corresponding to 4.0 g dry material were made up with Soerensen buffer (pH: 7) to 1 litre. To that mixture, 50 ml OECD recommended synthetic sewage feed were added.
- Incubation: sludged was incubated overnight at 20-25 °C under aeration.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 22 °C
- pH:
- Starting pH 7.7 in all vessels.
- Nominal and measured concentrations:
- 10 and 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass-flasks (> 500 ml)
- Aeration: aeration with 0.2 l/minute, instead of 0.5 l/minute to prevent possible foam development.
- No. of vessels per concentration: 1 replicate
- No. of vessels per control: 2 replicates
- Synthetic sewage per vessel: 16 ml
- Activated sludge amount: 200 ml
MEASUREMENTS
After exposure period, the first sample was taken for oxygen measurement over a period up to 10 minutes. Thereafter, in 15 minutes intervals, the rest of the samples were submitted to measurement of oxygen concentration.
During measurement, the sample was not aerated but cotinuously stirred on a magnetic stirrer. The apparatus used for oxygen measurements was the WTW OXI 91.
VALIDATION OF THE TESTS
The tests are validated by testing a known standard, i.e.3,5-dichlorophenol. The related IC50-value should be in the range of 5 - 30 mg/l.
Furthermore, the variation between the two control samples (start, end) should not be greater than 15 %. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- The aeration of the test mediun with test article caused the solution to foam. The foam was coloured. Under these conditions, the respiration rate of the sludge was moderately inhibited (25.1 %) at the highest concentration of test item, i.e. 100 mg/l.
- Results with reference substance (positive control):
- At a concentration of the standard of 10 mg/l, the respiration rate was highly inhibited by 56.7 %. The IC50 was found to be about 15.1 rng/l. Almost complete inhibition (86.0 %) was observed at 50 mg/l.
Any other information on results incl. tables
Influence of test item and reference control on oxygen consumption of activated sludge.
Vessel | Oxygen consumption (mg O2/l/min) | Inhibition (%) | Starting pH |
Control | 0.88 | -- | 7.7 |
Control | 0.83 | -- | 7.7 |
Mean |
0.85 ± 0.025 (5.7 %) | ||
Test item 10 mg/l | 0.79 | 7.6 | 7.7 |
Test item 100 mg/l | 0.64 | 25.1 | 7.7 |
3,5-dichlorophenol 3.2 mg/l | 1.04 | -21.6 | 7.7 |
3,5-dichlorophenol 10 mg/l | 0.37 | 56.7 | 7.7 |
3,5-dichlorophenol 50 mg/l | 0.12 | 86.0 | 7.7 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- the IC50-value of positive control resulted in the range of 5 - 30 mg/l and the variation between the two control samples was lower than 15 %
- Conclusions:
- IC50 (3h) > 100 mg/l
- Executive summary:
The inhibitory effect of test item on aerobic wastewater bacteria was investigated in a respiration test. The test article showed only a moderate inhibition (25.1 %) of the respiration rate up to a concentration of 100 mg/l.
The IC50 was determined to be higher than 100 mg/l.
Conclusion
IC50 (3h) > 100 mg/l
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