Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 270-616-4 | CAS number: 68459-67-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23 Mar - 25 Mar 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions (no purity, no information on dissolved oxygen at test end)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- adopted 1984
- Deviations:
- yes
- Remarks:
- dissolved oxygen at test end is not reported
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct dispersion of 2 g test material in 2 L of reconstituted water to give a stock solution from which dilutions were made to obtain the test series. - Test organisms (species):
- Daphnia magna
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 270 mg/L as CaCO3
- Test temperature:
- 21 °C
- pH:
- 7.8 ± 0.2
- Dissolved oxygen:
- Medium was air-saturated at test start.
- Nominal and measured concentrations:
- Nominal: 0, 10, 18, 32, 56, 100, 180, 320, 560 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 200 mL
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water was prepared from the following mineral salt stock solutions that were diluted 1:4 in deionised water: 11.76 g/L CaCl2*2H2O, 4.93 g/L MgSO4*7H2O, 2.59 g/L NaHCO3, 0.23 g/L KCl. The medium was aerated until oxygen saturation was reached, pH was adjusted to 7.8 ± 0.2 with NaOH or HCl if necessary.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mobility was observed after 24 h and 48 h.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: approx. 1.8
- Range finding study: yes
- Results used to determine the conditions for the definitive study: The range finding study indicated a 48 h NOEC and a 48 h EC50 would be obtained within the range of the concentrations chosen. - Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 200 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL = 170 - 250 mg/L
- Details on results:
- - Mortalities in the control vessels: None
- The results of the test solution analysis showed an increasing carbon concentration with increasing nominal test concentration with the exception of the 10 mg/L test group. This erroneous value was considered to be due to sampling error and/or analytical variation. Also there was no significant change in carbon concentration observed over 48 h exposures period. The results were taken to demonstrate the stability of the test concentrations employed during the period of exposure.
Reference
Table 1: Additional information on effect concentrations
Duration |
Endpoint |
Effect conc. |
Nom./Meas. |
Conc. based on |
Basis for effect |
Remarks |
24 h |
EC50 |
330 mg/L |
nominal |
test mat. |
mobility |
95% CL = 270 – 410 mg/L |
24 h |
NOEC |
56mg/L |
nominal |
test mat. |
mobility |
|
48 h |
NOEC |
56 mg/L |
nominal |
test mat. |
mobility |
|
Table 2: Cumulative Immobilisation Data for D. magna Exposed for 48 h to the test substance (R = Replicate)
Nominal Concentration of test substance (mg/L) |
Cumulative immobilised Daphnia (initial population: 10 per replicate) |
|||||||
24 h |
48 h |
|||||||
R1 |
R2 |
Total |
% |
R1 |
R2 |
Total |
% |
|
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
10 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
18 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
32 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
56 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
1 |
1 |
2 |
10 |
1 |
2 |
3 |
15 |
180 |
2 |
2 |
4 |
20 |
5 |
4 |
9 |
45 |
320 |
3 |
4 |
7 |
35 |
6 |
7 |
13 |
65 |
560 |
8 |
7 |
15 |
75 |
10 |
10 |
20 |
100 |
1000 |
10 |
10 |
20 |
100 |
10 |
10 |
20 |
100 |
Table 3: Results from Total Organic Carbon (TOC) Analysis
Samples |
Nominal Concentration (mg/L) |
Concentration of TOC corrected for control (mg C/L) |
0 h |
10 |
17.576 |
32 |
15.296 |
|
100 |
48.266 |
|
320 |
157.746 |
|
1000 |
518.246 |
|
48 h |
10 |
11.951 |
32 |
13.181 |
|
100 |
43.841 |
|
320 |
147.851 |
|
1000 |
490.051 |
Description of key information
The study was conducted according to OECD 202.
LC50 (48h) = 200 mg/L
NOEC (48h) = 56 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 200 mg/L
Additional information
The study with Castor oil, ester with glycerol (CAS 68459-67-6) was performed according to OECD 202 (GLP compliance not specified) under static conditions using the freshwater flea Daphnia magna as test organism. The daphnids were exposed to nominal concentrations of 0, 10, 18, 32, 56, 100, 180, 320, 560 and 1000 mg/L over a period of 48h. EC50 (48h) was determined to be 200 mg/L. The highest test concentration showing no adverse effects (NOEC) was 56 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.