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EC number: 226-009-1 | CAS number: 5216-25-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- From December 14 to January 20, 1983
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 984
- Report date:
- 1984
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 (Acute Toxicity (Oral))
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- α,α,α,4-tetrachlorotoluene
- EC Number:
- 226-009-1
- EC Name:
- α,α,α,4-tetrachlorotoluene
- Cas Number:
- 5216-25-1
- Molecular formula:
- C7H4Cl4
- IUPAC Name:
- 1-chloro-4-(trichloromethyl)benzene
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Hoechst AG, kastengrund, SPF-Zucht
- Weight at study initiation: mean of 184g for male (min: 170g, max: 189g) and of 180f for female (min: 167g, max: 194g)
- Fasting period before study: 16 hour prior the test and two after the exposure
- Housing: five rats by cage (type 4) in a ventilated room. woodchips on the ground of the cages
- Diet (e.g. ad libitum): granules Altromin 1324 ad libitum
- Water (e.g. ad libitum): tap water in drinking flasks ad libitum
- Acclimation period: five days prior the test
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 2 °C
- Humidity (%): 55 +/- 10 %
- Photoperiod (hrs dark / hrs light): 12 hours dark/light alternance
No further information
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: sesame oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 75 %
- Amount of vehicle (if gavage): from 1.25
- Justification for choice of vehicle: hydrolyzed in water and available vehicle
MAXIMUM DOSE VOLUME APPLIED: 3.2 mL/kg bw (corresponding to 800 mg/kg bw of test substance)
No further information - Doses:
- For both sexes: 315 (only female), 500, 630 and 800 mg/kg bw
- No. of animals per sex per dose:
- 5 rats (male and female) per dose except for the dose at 315 mg/kg bw (for female only)
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: daily observations but once a week for the weighing
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs and gross pathology
No further information - Statistics:
- LD50 were established by a probit analysis (method from Lindner and Weber) and confidence intervals were established with the method of Fieller or Sidak by a software.
LD50 for males and females were compared to check whether a statistical difference could be found between both sexes.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 652 mg/kg bw
- 95% CL:
- >= 518 - <= 844
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 581 mg/kg bw
- 95% CL:
- >= 442 - <= 724
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 614 mg/kg bw
- 95% CL:
- >= 528 - <= 706
- Mortality:
- At the end of the observation period (14 days):
- three males were dead when exposed to 630 mg/kg bw
- four males were dead when exposed to 800 mg/kg bw
- two females were dead when exposed to 500 mg/kg bw
- two females were dead when exposed to 630 mg/kg bw
- all females (5) were dead when exposed to 800 mg/kg bw - Clinical signs:
- After one and two days, both sexes rats exhibited adverse effects on their breathing, locomotion and behavioral posture. Myosis was also observed. At the highest tested dose in males, there was an increase of reflexes and lacrimation (blood colored tears).
- Body weight:
- Surviving male rats exhibited a decrease of their body weight after seven days
- Gross pathology:
- In both sexes, liver was noticed to be bright and spotted, the stomachal content was clear and no food was found in the big intestine. At the highest tested dose, the spleen was also bright. In females, liver lobes were scattered and in males, surrenales were dark brown and the small intestine was filled with a yellowish-orange mucus.
All surviving rats (in both sexes) were killed after 14 days of observation and exhibited no effects after a gross pathology exmination. - Other findings:
- No further information
Any other information on results incl. tables
In the following tables, the details on body weight variations of rats exposed to various concentrations of 4 -Cl-benzotrichloride are given:
Body weight of female rats exposed orally to 315 mg/kg bw of 4-Cl-benzotrichloride | |||||||
Animal number | initial weight (g) | weight after 7 days | weight after 14 days | ||||
g | % | g | % | ||||
1 | 176 | 196 | +11 | 215 | +22 | ||
2 | 171 | 192 | +12 | 214 | +25 | ||
3 | 180 | 199 | +11 | 198 | +10 | ||
4 | 176 | 200 | +14 | 201 | +14 | ||
5 | 175 | 193 | +10 | 201 | +15 |
Body weight of male rats exposed orally to 500 mg/kg bw of 4-Cl-benzotrichloride | |||||||
Animal number | initial weight (g) | weight after 7 days | weight after 14 days | ||||
g | % | g | % | ||||
1 | 189 | 204 | +8 | 249 | +32 | ||
2 | 183 | 168 | -8 | 224 | +22 | ||
3 | 170 | 156 | -8 | 217 | +28 | ||
4 | 181 | 181 | 0 | 232 | +28 | ||
5 | 184 | 209 | +14 | 252 | +37 |
Body weight of female rats exposed orally to 500 mg/kg bw of 4-Cl-benzotrichloride | |||||||
Animal number | initial weight (g) | weight after 7 days | weight after 14 days | ||||
g | % | g | % | ||||
1 | 178 | dead | 2d p.a | ||||
2 | 181 | 201 | +11 | 206 | +14 | ||
3 | 183 | 205 | +12 | 197 | +8 | ||
4 | 189 | dead | 2-3d p.a | ||||
5 | 181 | 204 | +13 | 204 | +13 |
Body weight of male rats exposed orally to 630 mg/kg bw of 4-Cl-benzotrichloride | |||||||
Animal number | initial weight (g) | weight after 7 days | weight after 14 days | ||||
g | % | g | % | ||||
1 | 184 | dead | 0-1d p.a | ||||
2 | 188 | dead | 1-2d p.a | ||||
3 | 185 | 187 | +1 | 236 | +28 | ||
4 | 185 | 185 | 0 | 235 | +27 | ||
5 | 181 | dead | 0-1d p.a |
Body weight of female rats exposed orally to 630 mg/kg bw of 4-Cl-benzotrichloride | |||||||
Animal number | initial weight (g) | weight after 7 days | weight after 14 days | ||||
g | % | g | % | ||||
1 | 174 | dead | 3-4d p.a | ||||
2 | 187 | dead | 0-1d p.a | ||||
3 | 194 | 200 | +3 | 231 | +19 | ||
4 | 192 | 209 | +9 | 209 | +9 | ||
5 | 189 | 199 | +5 | 204 | +8 |
Body weight of male rats exposed orally to 800 mg/kg bw of 4-Cl-benzotrichloride | |||||||
Animal number | initial weight (g) | weight after 7 days | weight after 14 days | ||||
g | % | g | % | ||||
1 | 188 | dead | 0-1d p.a | ||||
2 | 187 | dead | 0-1d p.a | ||||
3 | 186 | dead | 0-1d p.a | 231 | +19 | ||
4 | 182 | dead | 0-1d p.a | 209 | +9 | ||
5 | 182 | 198 | +9 | 251 | +38 |
Body weight of female rats exposed orally to 800 mg/kg bw of 4-Cl-benzotrichloride | |||||||
Animal number | initial weight (g) | weight after 7 days | weight after 14 days | ||||
g | % | g | % | ||||
1 | 174 | dead | 1d p.a | ||||
2 | 179 | dead | 0-1d p.a | ||||
3 | 164 | dead | 0-1d p.a | ||||
4 | 178 | dead | 0-1d p.a | ||||
5 | 167 | dead | 2-3d p.a |
In the following tables, the details on mortality of rats exposed to various concentrations of 4 -Cl-benzotrichloride are given:
Lethality table for female rats exposed orally to 315 mg/kg bw of 4-Cl-benzotrichloride | |||||||||||
Time after the application | from | 0 | 10 min | 30 min | 1 hour | 2 hour | 4 hour | 1 day | 2 days | 3 days | 5 days |
to | 10 min | 30 min | 60 min | 2 hour | 4 hour | 6 hour | 4 days | 14 days | |||
Lethality of rats | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 |
Clinical symptoms table for male rats exposed orally to 500 mg/kg bw of 4-Cl-benzotrichloride | ||||||||||||
Time after the application | from | 0 | 10 min | 30 min | 1 hour | 2 hour | 4 hour | 1 day | 2 days | 3 days | 4 days | 5 days |
to | 10 min | 30 min | 60 min | 2 hour | 4 hour | 6 hour | 14 days | |||||
Lethality of rats | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 |
Clinical symptoms table for female rats exposed orally to 500 mg/kg bw of 4-Cl-benzotrichloride | ||||||||||||
Time after the application | from | 0 | 10 min | 30 min | 1 hour | 2 hour | 4 hour | 1 day | 2 days | 3 days | 5 days | 6 days |
to | 10 min | 30 min | 60 min | 2 hour | 4 hour | 6 hour | 4 days | 14 days | ||||
Lethality of rats | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 1/5 | 2/5 | 2/5 | 2/5 |
Lethality table for male rats exposed orally to 630 mg/kg bw of 4-Cl-benzotrichloride | |||||||||||
Time after the application | from | 0 | 10 min | 30 min | 1 hour | 2 hour | 4 hour | 1 day | 2 days | 3 days | 5 days |
to | 10 min | 30 min | 60 min | 2 hour | 4 hour | 6 hour | 4 days | 14 days | |||
Lethality of rats | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 2/5 | 3/5 | 3/5 | 3/5 |
Clinical symptoms table for female rats exposed orally to 630 mg/kg bw of 4-Cl-benzotrichloride | ||||||||||||
Time after the application | from | 0 | 10 min | 30 min | 1 hour | 2 hour | 4 hour | 1 day | 3 days | 4 days | 5 days | 6 days |
to | 10 min | 30 min | 60 min | 2 hour | 4 hour | 6 hour | 2 days | 14 days | ||||
Lethality of rats | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 1/5 | 2/5 | 2/5 | 2/5 | 2/5 |
Clinical symptoms table for male rats exposed orally to 800 mg/kg bw of 4-Cl-benzotrichloride | ||||||||||||
Time after the application | from | 0 | 10 min | 30 min | 1 hour | 2 hour | 4 hour | 1 day | 2 days | 3 days | 4 days | 5 days |
to | 10 min | 30 min | 60 min | 2 hour | 4 hour | 6 hour | 14 days | |||||
Lethality of rats | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 4/5 | 4/5 | 4/5 | 4/5 | 4/5 |
Clinical symptoms table for female rats exposed orally to 800 mg/kg bw of 4-Cl-benzotrichloride | ||||||||||
Time after the application | from | 0 | 10 min | 30 min | 1 hour | 2 hour | 4 hour | 1 day | 2 days | 3 days |
to | 10 min | 30 min | 60 min | 2 hour | 4 hour | 6 hour | ||||
Lethality of rats | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 4/5 | 4/5 | 5/5 |
Applicant's summary and conclusion
- Interpretation of results:
- other: Category 4 based on CLP criteria
- Conclusions:
- LD50 = 614 mg/kg bw .
- Executive summary:
The authors tested the acute oral toxicity of 4-chloro-benzotrichloride (CAS n° 5216-25-1) according to the former OECD guideline 401. They administered one oral dose ranging from 315 mg/kg bw to 800 mg/kg bw with a gastric probe to wistar rats (both sexes) . They followed the mortality, performed clinical examinations and gross pathology on dead rats (surviving rats killed) over an observation period of fourteen days.
At the end of the experiment, the authors estimated by probit analysis a LD50 of 652 mg/kg bw for male rats and a LD50 of 581 mg/kg bw for female rats. The mortality was correlated with well documented clinical and gross pathology observations (e.g: breathing disruption, signs of anxiety...). After statistical analysis, the authors concluded that the LD50 for wistar rats in the test conditions was estimated at 614 mg/kg bw.
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