4-benzoylbenzene-1,2,3-triyl tris(6-diazo-5,6-dihydro-5-oxonaphthalene-1-sulphonate)

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11 Feb - 3 Mar 1982

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Version / remarks:

adopted in 1987

Deviations:

not specified

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-benzoylbenzene-1,2,3-triyl tris(6-diazo-5,6-dihydro-5-oxonaphthalene-1-sulphonate)

EC Number:

227-030-9

EC Name:

4-benzoylbenzene-1,2,3-triyl tris(6-diazo-5,6-dihydro-5-oxonaphthalene-1-sulphonate)

Cas Number:

5610-94-6

Molecular formula:

C₄₃H₂₂N₆O₁₃S₃

IUPAC Name:

4-benzoylbenzene-1,2,3-triyl tris(6-diazo-5,6-dihydro-5-oxonaphthalene-1-sulphonate)

Test material form:

solid

Test animals

Species:

rat

Strain:

Wistar

Remarks:

Hoe: WISKf (SPF71)

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: in-house breeding (Hoechst Aktiengesellschaft, Pharma Forschung, Toxikologie, Germany)
- Weight at study initiation: 186 - 202 g (range)
- Fasting period before study: animals were fasted for 16 h prior to and 2 h after administration
- Housing: group caged in plastic cages, on softwood granulate
- Diet: ALTROMIN 1324 (Altromin GmbH, Lage/Lippe, Germany), ad libitum
- Water: tap water, ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: starch solution

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 25%

Doses:

5000 mg/kg bw

No. of animals per sex per dose:

10 females

Control animals:

other: not required

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: animals were observed for mortality after gavage (no information in the study report on frequency), animals were weighed weekly
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Key result

Sex:

female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

No mortality occurred during the study period.

Clinical signs:

2 days after application all animals showed yellow faeces (no information given in the study report about duration).

Body weight:

All animals showed the body weight gain expected for this species and strain during the observation period.

Gross pathology:

No treatment-related findings were identified during the examination.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The LD50 of the test item was greater than 5000 mg/kg bw after oral administration to rats.

Executive summary:

The acute oral toxicity of the test item was investigated in a group of five male and five female CD rats at a dosage of 5000 mg/kg bw. The animals were starved overnight prior to dosing. The test material was administered at a constant volume-dosage of 20 mL/kg in 0.5% w/v methylcellulose in purified water. 

Mortality and signs of reaction to treatment were recorded during a subsequent 14-day observation period. The animals were killed on the following day and subjected to necropsy. There was no death and sign of reaction to treatment. All animals achieved expected bodyweight gains and necropsy findings were unremarkable. 

Under the conditions of this study, the acute oral median lethal dosage (LD50) of the test material was greater than 5000 mg/kg bw.