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EC number: 419-210-2 | CAS number: 178452-71-6 OLIVE-GREEN JB 1170
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Version / remarks:
- Adopted February 24, 1987
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.5 (Acute Toxicity: Eye Irritation / Corrosion)
- Version / remarks:
- July 31, 1992
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Pentasodium bis{7-[4-(1-butyl-5-cyano-1,2-dihydro-2-hydroxy-4-methyl-6-oxo-3-pyridylazo)phenylsulfonylamino]-5'-nitro-3,3'-disulfonatonaphthalene-2-azobenzene-1,2'-diolato} chromate (III)
- EC Number:
- 419-210-2
- EC Name:
- Pentasodium bis{7-[4-(1-butyl-5-cyano-1,2-dihydro-2-hydroxy-4-methyl-6-oxo-3-pyridylazo)phenylsulfonylamino]-5'-nitro-3,3'-disulfonatonaphthalene-2-azobenzene-1,2'-diolato} chromate (III)
- Cas Number:
- 178452-71-6
- Molecular formula:
- C66H48CrN16Na5O28S6
- IUPAC Name:
- chromium(3+) pentasodium bis(7-{4-[2-(1-butyl-5-cyano-2-hydroxy-4-methyl-6-oxo-1,6-dihydropyridin-3-yl)diazen-1-yl]benzenesulfonamido}-2-[2-(5-nitro-2-oxido-3-sulfonatophenyl)diazen-1-yl]-3-sulfonatonaphthalen-1-olate)
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Remarks:
- CRL : KBL
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
-Source: Charles River Stolzenseeweg 32-36 D-88353 Kisslegg
-Age at study initiation: 15 weeks
-Weight at study initiation:
male: 2.7 kg
females: 2.7 - 2.8 kg
-Housing: Individually in stainless steel cages with an automatic cleaning system, equipped with feed hoppers, drinking water bowls and wood for gnawing.
-Diet: pelleted standard Kliba 341, Batch no. 87/94 rabbit maintenance diet ("Kliba", Klingentalmuehle AG, CH-4303 Kaiseraugst), ad libitum.
-Water: community tap water from Itingen, ad libitum.
-Acclimation period: five days
ENVIRONMENTAL CONDITIONS
-Temperature: 20 ± 3 °C
-Humidity: 40-70 %
-Air changes: 10-15 air changes per hour
-Photoperiod: 12 hours cycle dark/light
-Other: music during the light period
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes
- Amount / concentration applied:
- TEST MATERIAL
-Amount: 0.1 g - Duration of treatment / exposure:
- single injection
- Observation period (in vivo):
- 1, 24, 48 and 72 hours and 7, 14 and 21 days after administration
- Number of animals or in vitro replicates:
- 1 male and 2 females
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing: no
OBSERVATIONS
The eyes of each animal were examined for ocular irritation 1, 24, 48 and 72 hours, as well as 7, 14 and 21 days after administration. Any findings ascertained during examination were recorded and the numerical irritation scores were assigned thereafter based upon the severity of the individual findings. Moreover, Viability/Mortality and Clinical Signs were observed daily during the observation period. Also, body weights were observed the start of acclimatization, on day 1 of test (application day) and at termination of observation.
SCORING SYSTEM:
CORNEA
- Opacity: degree of density (area most dense taken for reading)
No ulceration or opacity: 0
Scattered or diffuse areas of opacity (other than slight dulling of normal lustre), details of iris clearly visible: 1
Easily discernible translucent area, details of iris slightly obscured: 2
Nacreous areas, no details of iris visible, size of pupil barely discernible: 3
Opaque cornea, iris not discernible through the opacity: 4
- Area of cornea affected
No ulceration or opacity: 0
One quarter or less but not zero: 1
Greater than one quarter, but less than half: 2
Greater than half, but less than three quarters: 3
Greater than three quarters, up to whole area: 4
IRIS
Normal: 0
Markedly deepened rugae, congestion, swelling, moderate circumcorneal hyperaemia, or injection, any of these or any combination thereof, iris still reacting to light (sluggish reaction is positive): 1
No reaction to light, haemorrhage, gross destruction (any or all of these): 2
CONJUNCTIVAE
-Redness: (refers to palpebral and bulbar conjunctivae, excluding cornea and iris).
Blood vessels normal
Some blood vessels definitely hyperaemic (injected): 1
Diffuse, crimson colour, individual vessels not easily discernible: 2
Diffuse beefy red: 3
-Chemosis: lids and/or nictating membrane
No swelling: 0
Any swelling above normal (includes nictating membrana): 1
Obvious swelling with partial eversion of lids: 2
Swelling with lids about half closed: 3
Swelling with lids more than half closed: 4
-Discharge:
No discharge: 0
Any amount different from normal (does not include small amounts observed in inner canthus of
normal animals): 1
Discharge with moistening of the lids and hairs, just adjacent to lids: 2
Discharge with moistening of the lids and hairs, in a considerable area around the eye: 3.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- animal: #1, #2, #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Irritation parameter:
- iris score
- Basis:
- animal: #1, #2, #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Irritation parameter:
- conjunctivae score
- Basis:
- animal: #1, #2, #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Irritation parameter:
- chemosis score
- Basis:
- animal: #1, #2, #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Irritant / corrosive response data:
- CORROSION
No corrosion of the cornea was observed at any of the reading times. - Other effects:
- COLORATION
Green staining of the conjunctivae by the test substance persisted in both surviving animals until termination. Staining of the sclera was reversible in both animals within the 21-day observation period, whereas staining of the cornea persisted in one of the two animals up to 21 days.
VIABILITY / MORTALITY AND CLINICAL SIGNS
No clinical signs of systemic toxicity were observed in the animals during the test and observation period. Male rabbit no. 4 was found dead in its cage on the morning of test day 7. This finding was considered to be a spontaneous death unrelated to the treatment with the test substance.
BODY WEIGHTS
The body weight of the animals was within the normal range of variability.
NECROPSY
Male rabbit no. 4 was found dead on the morning of test day 7 and was subsequently necropsied. Dark red discoloration of the lung lobes was noted, but this finding was considered to be unrelated to the treatment with the test substance.
Applicant's summary and conclusion
- Interpretation of results:
- other: Classified according to the CLP Regulation (EC n. 1272/2008) Eye Dam. 1
- Conclusions:
- The test item induced irreversible damage to the rabbit eye, as the green coloured staining of the conjunctivae by the test item persisted throughout the observation period.
- Executive summary:
The eye irritation test was performed to the rabbit eye according to OECD Guidelines 405 (1987) and the method B.5 of EEC-Directive 92/69 EEC. 0.1 g of test item were installed into the left eye of 3 young adult New Zealand White rabbits (1 male and 2 females). The treated eyes were not rinsed after the application. Scoring of irritation effects was performed after test item application at 1, 24, 48 and 72 hour, as well as 7, 10, 14, 17 and 21 day.
Animal #1 was found dead on day 7, but the death was considered to be not treatment-related. Mean values at 24, 48 and 72 hours for iris, corneal opacity and the conjunctivae (redness and chemosis) were 0.00 in all animals. No corrosion was observed at any of the measuring intervals. The test item caused dark green staining of cornea, conjunctivae and sclera in all animals at the 1-hour reading. Light green staining was then observed in the following readings. For animal #1 staining of cornea, conjunctivae and sclera persisted until its death. For animals #2 and #3 staining of the conjunctivae persisted up to 21 days. Staining of the sclera was reversible within 14 days (animal #2) and 24 hours (animal #3). Staining of the cornea was still present in animal #2 after 21 days, whereas in animal #3 was reversible on day 21.
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