(Z)-hex-3-enyl isobutyrate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July 21, 2011 to October 04, 2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

yes

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(Z)-hex-3-enyl isobutyrate

EC Number:

255-424-0

EC Name:

(Z)-hex-3-enyl isobutyrate

Cas Number:

41519-23-7

Molecular formula:

C10H18O2

IUPAC Name:

(Z)-hex-3-enyl isobutyrate

Specific details on test material used for the study:

TEST MATERIAL
Name ( stated on the report) : Hexenyl-3-cis Isobutyrate
Appearance: Colourless to pale yellow liquid
Batch: VE00153050
Expiry date: April 22, 2012

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (Bois-de-Bay, Satigny, Switzerland) was used.
The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

30 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance

Reference substance:

other: Sodium benzoate (Fluka, Buchs, Switzerland, Art. No. 71300), purity : min. 99.0 %

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

78

Sampling time:

28 d

Details on results:

The 10-day window criterion is also fulfilled (19 % biodegradation on day 2 and 64 % on day 12).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Hexenyl-3-cis Isobutyrate undergoes 78 % biodegradation after 28 days in the test conditions.
The 10-day window criterion is also fulfilled (19 % biodegradation on day 2 and 64 % on day 12).
Hexenyl-3-cis Isobutyrate did not inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.
Thus, Hexenyl-3-cis Isobutyrate should be regarded as readily biodegradable according to this test.

Executive summary:

The Ready Biodegradability of Hexenyl-3-cis Isobutyrate was determined by the Manometric Respirometry Test.

Hexenyl-3-cis Isobutyrate undergoes 78 % biodegradation after 28 days in the test conditions.

The 10-day window criterion is also fulfilled (19 % biodegradation on day 2 and 64 % on day 12).

Hexenyl-3-cis Isobutyrate did not inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.

Thus, Hexenyl-3-cis Isobutyrate should be regarded as readily biodegradableaccording to this test.