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EC number: 203-464-4 | CAS number: 107-12-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21-24/4/1981
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- no analytical meaurement of tested material concentrations were perfomed
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- no analitycal analysis to verify concentration of chemical. only two replicates of 10 daphnids
- Principles of method if other than guideline:
- - Principle of test:
The acute toxicity of Propionitrile to Daphnia magna was assessed using the methods outlined by the Commitee on Methods for Toxicity Tests with Aquatic Organisms.
- Short description of test conditions: Water quality parameters of temperature, dissolved oxygen and pH were measured at the termination of the test and were within acceptable limits. Static Daphnia bioassay was conducted in 250 ml glass beakers containing 200 ml of well water. These vessels were kept at 20°C (±1.0). The photoperiod was controlled to give 16 hours daylight and 8 hours darkness.
- Parameters analysed / observed: 48 h immobilization - GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Propiononitrile
- EC Number:
- 203-464-4
- EC Name:
- Propiononitrile
- Cas Number:
- 107-12-0
- Molecular formula:
- C3H5N
- IUPAC Name:
- propanenitrile
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch: Montesanto lot. #34
- Purity test date: not stated
- Purity: 96.1 %
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: 4 C
- Solubility and stability of the test substance in water: 9.94% at 20 C
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: dilution in deionized water
- Dilution volume: 100 ml deionized water
- Concentration: 20 mg/ml
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- Not applicable
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Test concentrations were prepared based on total compound received. Deionized water was used in the preparation of all working stock solutions.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: in-house culture
- Age of parental stock : <24 hours old)
- Feeding during test: not fed
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 255 ppm
- Test temperature:
- 21 °C
- pH:
- 7.8 to 8.3
- Dissolved oxygen:
- 8.6 to 8.7 mg/l
- Salinity:
- no data
- Conductivity:
- 50 µmhos/cm
- Nominal and measured concentrations:
- 100, 180, 320, 560 and 1000 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers
- Material, size, headspace, fill volume: 250 ml beakers containing 200 ml of well water
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates):2
TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: 48h
OTHER TEST CONDITIONS
- Photoperiod: 16/8
RANGE-FINDING STUDY
- Test concentrations: 1, 10 and 100 mg/l
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 250 mg/L
- Nominal / measured:
- estimated
- Basis for effect:
- mortality
- Remarks on result:
- other:
- Remarks:
- 95% C.I. 210-290 mg/l
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 310 mg/L
- Nominal / measured:
- estimated
- Basis for effect:
- mortality
- Remarks on result:
- other:
- Remarks:
- 95% C.I. 270-350 mg/l
- Details on results:
- See tables 1 and 2 in "any other information on results" and "overall remarks attachments" sections
- Results with reference substance (positive control):
- not applicable
- Reported statistics and error estimates:
- LC50 values and 95% confidence intervals were obtained by employing a computerized LC50 program developed by Stephan et. al. 1978 (A computer program for calculating an LC50 U. S. Environmental Protection Agency, Duluth, Minnesota, pre-publication manuscript, August, 1978.), performing the following statistical tests: binomial, moving average and probit tests. The results from the moving average or probit tests were used when there were two or more partial mortalities. If there weren't more than one partial mortality, the results from the binomial test were recorded.
Any other information on results incl. tables
Table 1. Raw data range-finding test
Test Conc. mg/l (ppm) |
24h |
48h |
||
Dead |
Observation |
Dead |
Observation |
|
Control (a) |
0 |
N |
0 |
N |
1 |
0 |
N |
0 |
N |
10 |
0 |
N |
0 |
N |
100 |
0 |
N |
0 |
N |
N= normal; LOE= loss of equilibrium; SUR= surfacing |
Table 2. Raw data definitive test
Test Conc. mg/l (ppm) |
24h |
48h |
||
Dead |
Observation |
Dead |
Observation |
|
Control (a) |
0 |
N |
0 |
N |
Control (b) |
0 |
N |
0 |
N |
100 (a) |
0 |
N |
0 |
N |
100 (b) |
0 |
N |
0 |
9N, 1 SUR |
180 (a) |
0 |
N |
1 |
9N |
180 (b) |
1 |
9N |
3 |
7N |
320 (a) |
4 |
4N, 2 SUR |
8 |
2N |
320 (b) |
6 |
4N |
7 |
3N |
560 (a) |
10 |
|
10 |
|
560 (b) |
10 |
|
10 |
|
1000 (a) |
10 |
|
10 |
|
1000 (b) |
10 |
|
10 |
|
N= normal; LOE= loss of equilibrium; Sur= surfacing |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of the test item to the freshwater Daphnia magna resulted in an estimated 48-hour LC50 value of 250 mg/L. The NOEC for Propionitrile was 100 mg/l after 48 hours.
- Executive summary:
The acute toxicity of Propionitrile to Daphnia magna was assessed using the methods outlined by the Commitee on Methods for Toxicity Tests with Aquatic Organisms which follows the guidelines of the OECD 202 standard for acute Daphnia tests. The study is reliable with restriction (Klimisch 2) and with GLP certification. Water quality parameters of temperature, dissolved oxygen and pH were measured at the termination of the test and were within acceptable limits; however, no analytical measurement of test material concentrations were performed. Test concentrations of a nominal 100, 180, 320, 560 and 1000mg/l were tested. The estimated 48-hour LC50 (95% C. I.) for propionitrile was 250 mg/1 (210 -290 mg/1) and a 48 hour NOEC of 100mg/l.
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