3,7-bis(diethylamino)phenoxazin-5-ium nitrate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Aug - Sep 1990

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

1984

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3,7-bis(diethylamino)phenoxazin-5-ium acetate

EC Number:

279-349-8

EC Name:

3,7-bis(diethylamino)phenoxazin-5-ium acetate

Cas Number:

79916-07-7

Molecular formula:

C20H26N3O.C2H3O2

IUPAC Name:

3,7-bis(diethylamino)phenoxazin-5-ium acetate

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Analytical measurements of the test article were performed in the stock solution and the test media of the nominal test concentrations 0.0625, 0.25 and 1 mg test article/L at the start and the end of the test by sampling freshly prepared test media and media, which were incubated under the same conditions as in the test itself during test duration.

The samples were stored deep-frozen (-20°C) until the analysis was performed.

Test solutions

Vehicle:

no

Details on test solutions:

A stock solution was prepared by dissolving the test article in test water (1 g/L). The stock solution was diluted in a series of sequential dilutions with test water and adequate volumes of these stock solutions were added to obtain the following nominal test concentrations: 0.0625, 0.125, 0.25, 0.50 and 1.0 mg/L and a control (test water without any additions).

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The study was performed with young Daphnia magna (6-24 h old), bred in the laboratories of RCC under standardized conditions. The parental Daphnia were maintained in the same reconstituted water as in the test for at least 24 h prior to the start of the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.5 mmol/L CaCO3

Test temperature:

20.5 - 20.6°C

pH:

7.9 - 8.0

Dissolved oxygen:

9.0 - 9.2 mg/L

Nominal and measured concentrations:

Nominal: 0 (control), 0.0625, 0.125, 0.250, 0.500, 1.00 mg/L
Measured (geometric mean): 0 (control), 0.0679 (109%), 0.236 (94%), 0.955 (96%)

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beaker
- Material, size, headspace, fill volume: 100 mL total volume, 50 mL fill volume
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 light / 8 darkness
- Light intensity: 200 - 600 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility at 24 and 48 h

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.239 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CI: 0.203 - 0.280 mg/L

Details on results:

0% immbolity in control
Analytical verification of test item within 80 - 120% recovery range

Reported statistics and error estimates:

Probit analysis

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test item was found to be acutely toxic to Daphnia magna. The 48-h EC50 was determined at 0.239 mg/L (nominal, analytically verified.)