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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Study reported in 1975
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Remarks:
(only basic data given)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1975
Report date:
1975

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Version / remarks:
adopted Feb 1987
Deviations:
yes
Remarks:
(only basic data given)
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3-acetyl-6-methyl-2H-pyran-2,4(3H)-dione
EC Number:
208-293-9
EC Name:
3-acetyl-6-methyl-2H-pyran-2,4(3H)-dione
Cas Number:
520-45-6
Molecular formula:
C8H8O4
IUPAC Name:
3-acetyl-6-methyl-2H-pyran-2,4(3H)-dione
Test material form:
solid: particulate/powder
Specific details on test material used for the study:
Dehydroacetic acid

Test animals

Species:
rat
Strain:
other: albino
Remarks:
200-300 grams body weight
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Fasting period before study: 24 h
- Housing: 5 animals of the same sex per common cage
- Diet: ad libitum
- Water: ad libitum

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
propylene glycol
Doses:
1000, 1600, 2000, 2500, 3200, 4000, 5000 and 6400 g/kg (males)
1000, 1250, 1600, 2000, 2500, 3200, 4000, 5000 and 6400 g/kg (females)
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations: daily

Results and discussion

Effect levelsopen allclose all
Key result
Sex:
female
Dose descriptor:
LD50
Effect level:
1 480 mg/kg bw
Based on:
test mat.
Key result
Sex:
male
Dose descriptor:
LD50
Effect level:
1 620 mg/kg bw
Based on:
test mat.
Mortality:
For details refer to Table 1 under "any other information on results"
Clinical signs:
other: All dose goups: lethargy 2000 mg/kg bw or more: gradual loss of motor control on the second day, magnified loss of motor control in further study progress, weight loss and debilitated survivors 5000 mg/kg or more: lack of motor control, hyperactive respo

Any other information on results incl. tables

Table 1: Acute oral toxicity

Dose Mortality Clinical signs
[mg/kg bw]
  N* N*
Males
1000 0/5 5/5
1600 2/5 5/5
2000 4/5 5/5
2500 5/5 5/5
3200 5/5 5/5
4000 5/5 5/5
5000 5/5 5/5
6400 5/5 5/5
Females
1000 0/5 5/5
1250 1/5 5/5
1600 3/5 5/5
2000 5/5 5/5
2500 5/5 5/5
3200 5/5 5/5
4000 5/5 5/5
5000 5/5 5/5
6400 5/5 5/5

*N = Number of animals

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Conclusions:
The LD50 values determined were 1620 mg/kg bw for the males and 1480 mg/kg bw for the females.
Executive summary:

An acute oral toxicity study was conducted in male and female rats. Dosages in the range 1000 to 6400 mg/kg bw were tested. The LD50 values determined were 1620 mg/kg bw for the males and 1480 mg/kg bw for the females. Clinical signs noted included: lethargy, lack of motor control, hyperactive responses, convulsions and hemorrhaging from the nose and mouth.