4'-methoxyacetoacetanilide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989 - 1990

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well performed OECD study with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4'-methoxyacetoacetanilide

EC Number:

226-615-6

EC Name:

4'-methoxyacetoacetanilide

Cas Number:

5437-98-9

Molecular formula:

C11H13NO3

IUPAC Name:

N-[4-(methoxyacetyl)phenyl]acetamide

Details on test material:

Purity: > 99 %

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrabärbling
- Strain: Hamilton-Buchanan
- Source: West Aquarium, 3422 Bad Lauterberg
- Length at study initiation: 1. test 2,8 +- 0.18 cm; 2. test 3,0 +-0,39 cm
- Feeding during test: no

ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions: same as test
- Type and amount of food: Tetra Min, Tetra Werke, Melle
- Feeding frequency: twice a day ad libitum

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

21,0 - 22,2 °C

pH:

7,6 - 8,2

Dissolved oxygen:

6,3 - 8,7 mg O2/L

Nominal and measured concentrations:

nominal (mg/L) measured (mg/L)
220 211
500 491

Details on test conditions:

TEST SYSTEM
- Test vessel: glass container (30 cm x 22 cm x 24 cm) calibrated to 10 liters
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2


OTHER TEST CONDITIONS
- Photoperiod: 12 hours illumination from upside
- Light intensity: about 700 Lux

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

220 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The LD50 is 331,7 mg/L.

Executive summary:

The test item was tested for acute toxicity to Brachydanio rerio according to OECD guideline 203 for 96 hours. Concentrations of 220 mg/L and 500 mg/L were applied . For each concentration 10 fish were used. The 220 mg/L-group showed only symptoms but no letality. The 500 mg/L-group showed symptoms and letality. Out of the results a LD50 value of 331,7 mg/L could be determined.