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EC number: 217-614-1 | CAS number: 1908-87-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016-06-06 - 2016-07-11 (experimental phase)
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- as of 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hess. Ministerium für Umwelt, Energie, Landwirtschaft und Verbraucherschutz, Mainzer Straße 80, D65189 Wiesbaden
- Specific details on test material used for the study:
- Stability under test conditions: A supporting chemical analysis was performed within this study.
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 20, 40, 80, 100 mg/L
- Sample storage conditions before analysis: The supporting analyses could not be performed on the same day of collection of the specimens. Therefore, these specimens from freshly prepared test solutions were stored deep-frozen, thawed over-night and then analysed. - Vehicle:
- not specified
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item was tested at concentrations 10 to 100 mg/L (main test) prepared in drinking water from the municipal drink water supply entering the institute. The drinking water used for the test refers to the OECD Guideline 203 and is controlled regularly (monthly). The test item was introduced into the water by direct weighing into the different treatments to give the final nominal concentrations.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): not stated - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebra fish
- Source: Aquahandel.de (Holtenser Weg 6a, 37186 Moringen)
- Length at study initiation (length definition, mean, range and SD): length ≈ 2.0 ±1.0 cm
ACCLIMATION
- Feeding frequency during acclimation: The fish were not fed 24 hr prior to testing.
FEEDING DURING TEST
The fish were not fed 24 hr prior to testing and during the whole test. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- none
- Hardness:
- 2.01 / 0.72 mmol/L (Ca2+ / Mg2+) (average Jan-Dec 2015)
1.84 / 0.69mmol/L (Ca2+ / Mg2+) (average Jan-Jul 2016) - Test temperature:
- 21.0 to 22.6 °C
- pH:
- pH of the test solutions was not adjusted.
pH = 7.81 - 8.64 - Dissolved oxygen:
- 8.0 - 8.4 mg/L
- Nominal and measured concentrations:
- Nominal: 0, 10, 20, 40, 80, 100 mg/L
Measured (after correction): 8.7-11.7, 20.1-21.9, 38.6-42.7, 71.1-77.4, 89.4-109.9 mg/L - Details on test conditions:
- TEST SYSTEM
- Aeration: The test solutions were aerated during the incubation period (96 hr) in order to verify an oxygen content of >60% of the saturation value during the study
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): daily
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water
- Intervals of water quality measurement: monthly
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : behaviour, mortality (daily)
TEST CONCENTRATIONS
- Test concentrations: 120 mg/L - Reference substance (positive control):
- not required
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- <= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: At 80 mg/L and 100 mg/L animals disimpassioned and partially at the bottom of the aquarium and increased movement of gills, lesser agile and swimming at the bottom
- Mortality of control: none
- Other adverse effects control: none stated
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- n/a
- Sublethal observations / clinical signs:
Results – Biological Part
Screening-Test (Concentrations successively tested)
Nominal Concentration
[mg Test Item per Liter of Tap Water]
Mortality [%] after
1d (3 hr)
2d
3d
4d
1201
33 (1 of 3)
67 (2 of 3)
67 (2 of 3)
67 (2 of 3)
1) Erroneously the technician used a wrong measuring cylinder resulting in a smaller final volume (2.5L instead of 3L) and thus in a higher concentration than intended in the study plan (120 instead of 100 mg/L). This represents a deviation of the study plan which is not considered to have an influence on the integrity of the study.
Accompanying Determinations (Screening-Test, semistatic Test Procedure):
Nominal conc. of Test Item [mg/L]
Incubation time[h]
pH-Value
O2-Content [mg O2/L]
Temperature [°C]
t01)
(t24hr)2)
t01)
(t24hr)2)
t01)
(t24hr)2)
120
0
24
48
72
96
8.12
8.12
8.10
8.11
-
-
8.53
8.55
8.68
8.61
8.5
8.4
8.4
8.3
-
-
8.3
8.4
8.5
8.3
21.8
21.2
21.1
21.4
-
-
21.2
21.1
21.2
21.1
1)Values in the fresh test solutions, respectively;2)after 24 hr of incubation with the fish
LC0(after 96 hr) < 120 mg/L of tap-water
LC50(after 96 hr) < 120 mg/L of tap-water
LC100(after 96 hr) > 120 mg/L of tap-water
Nominal Concentration
[mg Test Item per Liter of Tap Water]
Mortality [%] after
0 d
2d
3d
4d
12.5 (10)1
0 (0 of 3)
0 (0 of 3)
0 (0 of 3)
0 (0 of 3)
1) Erroneously, within the first three days the technician used a wrong measuring cylinder resulting in a smaller final volume (2.5L instead of 3L) and thus in a higher concentration than intended in the study plan (12.5 instead of 10 mg/L). This represents a deviation of the study plan which is not considered to have an influence on the integrity of the study.
Resulting:
LC0(after 96h) > 10 mg/L of tap-water
LC50(after 96h) > 10 mg/L of tap-water
LC100(after 96h) > 10 mg/L of tap-water
Accompanying Determinations:
Nominal conc. of Test Item [mg/L]
Incubation time [hr]
pH-Value
O2-Content [mg O2/L]
Temperature [°C]
t01)
(t24hr)2)
t01)
(t24hr)2)
t01)
(t24hr)2)
12.5 (10)1
0
25
50.5
72
96
8.13
8.15
8.13
8.18
-
-
8.53
8.64
8.60
8.63
8.3
8.3
8.4
8.3
-
-
8.7
8.6
8.5
8.4
21.3
21.2
21.5
21.6
-
-
21.1
21.2
21.1
21.0
1)Values in the fresh test solutions, respectively;2)after 24 hr of incubation with the fish
Results-Main Test
Nominal Concentration
[mg Test Item per Liter of Tap Water]
Mortality [%] after
1d
2d
3d
4d (96 hr)
Control (0)
0 (0 of 7)
0 (0 of 7)
0 (0 of 7)
0 (0 of 7)
10
0 (0 of 7)
0 (0 of 7)
0 (0 of 7)
0 (0of 7)
20
0 (0 of 7)
0 (0 of 7)
0 (0 of 7)
0 (0 of 7)
40
0 (0 of 7)
0 (0 of 7)
0 (0 of 7)
0 (0 of 7)
80
0 (0 of 7)
0 (0 of 7)
0 (0 of 7)
0 (0 of 7)
100
0 (0 of 7)
0 (0 of 7)
0 (0 of 7)
14.3 (1 of 7)
Accompanying determinations (Main Test, semistatic Test Procedure):
Nominal conc. of Test Item [mg/L]
Incubation time[d]
pH-Value
O2-Content [mg O2/L]
Temperature [°C]
t01)
(t24hr)2)
t01)
(t24hr)2)
t01)
(t24hr)2)
Control (0)
0
1
2
3
4 (96hr)
8.09
7.81
8.03
8.13
-
-
8.56
8.64
8.63
8.58
8.2
8.4
8.2
8.4
-
-
8.4
8.3
8.3
8.3
21.8
21.3
21.5
21.4
-
-
22.6
21.9
22.5
22.3
10
0
1
2
3
4 (96hr)
8.10
7.81
8.09
8.09
-
-
8.57
8.35
8.61
8.54
8.2
8.3
8.3
8.4
-
-
8.4
8.3
8.2
8.3
21.7
21.2
21.5
21.0
-
-
22.5
21.9
22.4
22.3
20
0
1
2
3
4 (96hr)
8.11
0.03
8.10
8.09
-
-
8.58
8.63
8.61
8.60
8.2
8.2
8.3
8.3
-
-
8.2
8.3
8.2
8.3
22.0
21.3
21.1
21.4
-
-
22.5
21.9
22.5
22.3
40
0
1
2
3
4 (96hr)
8.12
8.05
8.10
8.09
-
-
8.57
8.49
8.55
8.54
8.2
8.3
8.3
8.4
-
-
8.2
8.3
8.3
8.3
22.1
21.7
21.1
21.7
-
-
22.6
21.9
22.6
22.3
80
0
1
2
3
4 (96hr)
8.12
8.03
8.09
8.09
-
-
8.56
8.55
8.48
8.50
8.0
8.2
8.3
8.3
-
8.3
8.3
8.2
8.2
22.2
21.2
21.3
21.1
-
-
22.5
22.0
22.5
22.3
100
0
1
2
3
4 (96hr)
8.13
8.05
8.11
8.09
-
-
8.64
8.65
8.47
8.50
8.1
8.3
8.3
8.2
-
8.3
8.3
8.3
8.2
22.2
21.1
21.3
21.2
-
-
22.5
21.9
22.5
22.4
1)Values in the fresh test solutions, respectively;2)after 24hr of incubation with the fish
Assumed Toxicity Values (after 96h):
On the Basis of the nominal Concentrations [mg/L]
LC10 156.7
95%-CL lower n.d.
upper n.d.
LC20 >100
95%-CL lower n.d.
upper n.d.
LC50 >100
95%-CL lower n.d.
upper n.d.
Mortality LOEC = 100
NOEC ≤ 100
Observations on the behaviour of the fish (Main Test):
Time (h)
Effects / Observations
1
At 80 mg/L and 100 mg/L animals disimpassioned and partially at the bottom of the aquarium and increased movement of gills
2
At 80 mg/L and 100 mg/L animals disimpassioned and partially at the bottom of the aquarium and increased movement of gills; animals at other test concentrations without symptoms
4 – 6
At 80 mg/L and 100 mg/L animals disimpassioned and partially at the bottom of the aquarium and increased movement of gills; animals at other test concentrations without symptoms
18 – 20
At 80 mg/L and 100 mg/L animals disimpassioned and partially at the bottom of the aquarium and increased movement of gills; animals at other test concentrations without symptoms
24
At 80 mg/L and 100 mg/L animals disimpassioned and partially at the bottom of the aquarium and increased movement of gills; animals at other test concentrations without symptoms
28 – 30
At 80 mg/L and 100 mg/L animals lesser agile and swimming at the bottom
42 – 44
At 80 mg/L and 100 mg/L animals lesser agile and swimming at the bottom, at 100 mg/L one fish without movements in one corner of the aquarium
48
At 80 mg/L and 100 mg/L animals lesser agile and swimming at the bottom, at 100 mg/L one fish with red discoloured head
48-66
At 80 mg/L and 100 mg/L animals lesser agile and swimming at the bottom, at 100 mg/L one fish with red discoloured head
72
At 80 mg/L and 100 mg/L animals lesser agile and swimming at the bottom, at 100 mg/L 2 fish apathetically and one of them disoriented
96
At 100 mg/L one fish dead and one without movements in one corner of the aquarium
Results of the Analytical Part (GC-MS)
The results of the supporting analyses can be summarised as follows:
Sample Designation
mg test item/L found
mg test item/L found after “procedural recovery” correction
% Recovery on basis of Nominal Concentrations
% Recovery on Basis of t0
Control time t0
n.d.
-
-
-
10 mg/L time t0
6.95
8.7 (a)
87.4
-
20 mg/L time t0
15.96
20.1 (a)
100.4
-
40 mg/L time t0
32.75
42.7 (b)
106.7
-
80 mg/L time t0
54.95
71.6 (b)
89.6
-
100 mg/L time t0
84.28
109.9 (b)
109.9
-
Control time t24 hr
n.d.
-
-
-
10 mg/L time t24 hr
6.97
8.8 (a)
87.7
100.3
20 mg/L time t24 hr
17.42
21.9 (a)
109.6
109.1
40 mg/L time t24 hr
29.63
38.6 (b)
96.6
90.5
80 mg/L time t24hr
54.50
71.1 (b)
88.8
99.2
100 mg/L time t24 hr
72.06
94.0 (b)
94.0
85.5
Control time t0’
n.d.
-
-
-
10 mg/L time t0’
8.58
11.7 (c)
117.2
-
20 mg/L time t0’
15.19
20.8 (c)
103.8
-
40 mg/L time t0’
34.01
41.7 (d)
104.3
-
80 mg/L time t0’
63.09
77.4 (d)
96.7
-
100 mg/L time t0’
76.46
93.8 (d)
93.8
-
Control time t24 hr’
n.d.
-
-
-
10 mg/L time t24 hr’
6.79
9.3 (c)
92.8
79.1
20 mg/L time t24 hr’
15.43
21.1 (c)
105.4
101.6
40 mg/L time t24 hr’
33.81
41.5 (d)
103.7
99.4
80 mg/L time t24 hr’
60.76
74.5 (d)
93.2
96.3
100 mg/L time t24 hr’
72.89
89.4 (d)
89.4
115.5
Spike 10 mg/L
("Procedural Recovery"), 1stSer.7.95 (a)
(10)
-
-
Spike 100 mg/L
("Procedural Recovery"), 1stSer.76.70 (b)
(100)
-
-
Spike 10 mg/L
("Procedural Recovery"), 2ndSer.7.32 (c)
(10)
-
-
Spike 100 mg/L
("Procedural Recovery"), 2ndSer.81.53 (d)
(100)
-
-
(a), (b),(c), (d) are the respective bases for the calculations with respect of the “procedural recoveries”.
As a result of the supporting analyse it can be stated that the results of the biological part should be based on the nominal concentrations applied because the necessary stability of ≥ 80% could be shown. The low % recovery on basis of the t0value at concentration 10 mg/L of the second series is due to the high recovery at t0. The geometric mean of t0and after 24 hr of that pair of values is >100% of the nominal concentration and thus, it can be stated that the fish were always in contact with >80% of the nominal concentrations applied, and therefore the results of the biological part shall be based on the nominal concentrations applied.
Validity of Results
The criteria of validity of results given by the OECD Guideline No. 203 (1992) were kept within this study. In the control no dead animals were observed. In the test solutions the oxygen concentration did not drop below a value of 60% of the saturation value (»5 mg O2/L).
Discussion
The test item was tested for acute toxicity towards fish according to OECD Guideline No. 203 (1992) using zebra fish. In order to investigate the influence of the test item towards the fish, the animals were exposed to up to 120 mg (screening) of the test item per litre of test solution. Under the conditions used for the test, no significant toxic effects of the test item towards fish were observed at 100 mg/L (main test). As a conclusion of the analytical part of this study, it can be stated that the concentrations of the test item remained sufficiently stable during incubation. Based on this, the reported effect concentrations should refer to the nominal concentrations tested. The following LC-values (effective concentrations) were determined accordingly:
On the Basis of the nominal Concentrations [mg/L]
LC10 156.7
95%-CL lower n.d.
upper n.d.
LC20 >100
95%-CL lower n.d.
upper n.d.
LC50 >100
95%-CL lower n.d.
upper n.d.
Mortality LOEC = 100
NOEC ≤ 100
The criteria of validity of results given by the OECD Guideline 203 were kept within this study. In the control no dead animals were observed. In the test solutions the oxygen concentration did not drop below a value of 60% of the saturation value (»5 mg O2/L).
- Validity criteria fulfilled:
- yes
- Conclusions:
- The study was performed according to OECD TG 203 with no relevant deviations, the results were so obtained via a scientifically reasonable method. Hence, there is no doubt that the obtained results are not reliable: Under the conditions used for the test, no significant toxic effects of the test item towards fish were observed at 100 mg/L (main test). As a conclusion of the analytical part of this study, it can be stated that the concentrations of the test item remained sufficiently stable during incubation. Based on this, the reported effect concentrations should refer to the nominal concentrations tested. The following LC-values (effective concentrations) were determined accordingly:
Mortality
LOEC = 100 mg/L
NOEC ≤ 100 mg/L
LC50 > 100 mg/L
Based on these results, the test item does not need to be classified as acute or chronic toxic to the aquatic environment. - Executive summary:
3-Methyl-thiazolidin-thion-2 was tested for acute toxicity towards fish according to OECD Guideline No. 203 (1992) using zebra fish. In order to investigate the influence of the test item towards the fish, the animals were exposed to up to 120 mg (screening) of the test item per litre of test solution. Under the conditions used for the test, no significant toxic effects of the test item towards fish were observed at 100 mg/L (main test). As a conclusion of the analytical part of this study, it can be stated that the concentrations of 3-Methyl-thiazolidin-thion-2 remained sufficiently stable during incubation. Based on this, the reported effect concentrations should refer to the nominal concentrations tested. The following LC-values (effective concentrations) were determined accordingly:
On the Basis of the nominal Concentrations [mg/L]
LC10 156.7
95%-CL lower n.d.
upper n.d.
LC20 >100
95%-CL lower n.d.
upper n.d.
LC50 >100
95%-CL lower n.d.
upper n.d.
Mortality LOEC = 100
NOEC ≤ 100
The criteria of validity of results given by the OECD Guideline 203 were kept within this study. In the control no dead animals were observed. In the test solutions the oxygen concentration did not drop below a value of 60% of the saturation value (»5 mg O2/L).
Reference
Description of key information
Acute toxicity to fish: LC50(96h) > 100 mg/L for Brachidanio rerio (OECD 203, GLP, semi-static)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
The result LC50(96h) > 100 mg/L does not trigger classification as harmful for the aquatic environment, neither acute nor chronic, according to Regulation (EC) 1272/2008.
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