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EC number: 914-475-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002-03-20 - 2002-04-28
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The test was performed according to relevant guidelines and compliant to GLP. However, the study is insufficiently documented and details crucial for calculating the theoretical oxygen demand are lacking. Duplicate measurements had not been performed. The test item is specified as "practically not soluble in water". However, no details are given how a stock solution of 400 mg/L had been prepared. The kind and amount of inoculum is not sufficiently described in the study. However, the results are plausible as degradation proceeded steadily in a linear manner and from day 17 to day 28 approaching a plateau.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- yes
- Remarks:
- duplicate measurements for inoculum blanks and test items had not been performed.
- Qualifier:
- according to guideline
- Guideline:
- other: Dissolved oxygen, DIN EN 25814 (G 22)
- Qualifier:
- according to guideline
- Guideline:
- other: ThOD (theoretical (chemical) oxygen depletion) DIN 38409-H 41
- Qualifier:
- according to guideline
- Guideline:
- other: Nitrate DIN EN ISO 10304-1 (D 19); Nitrite DIN EN 26777 (D 10);
- Qualifier:
- according to guideline
- Guideline:
- other: dissolved organic carbon (DOC), DIN EN 1484 (H 3)
- Principles of method if other than guideline:
- not applicable
- GLP compliance:
- yes
- Remarks:
- according to GLP-part II 2;7
Test material
- Reference substance name:
- Reaction mass of Fatty acids, montan-wax and Fatty acids, montan-wax, ethylene esters and Montan wax
- EC Number:
- 914-475-5
- Molecular formula:
- R-CH2-COOR1OOC-CH2-R mainly
- IUPAC Name:
- Reaction mass of Fatty acids, montan-wax and Fatty acids, montan-wax, ethylene esters and Montan wax
- Details on test material:
- Name: Licowax E
chemical name: ester of montanacids
active content: 100 %;
solubility: practically not soluble in water
appearance: Iight yellow solid
Solubility as determined in this study: DOC = 0.62 mg/L (dissolved organic carbon)
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
not applicable
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- local sewage work, secondary effluent; no further specification on type of sewage treatment plant or handling and amount of sludge used.
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 4 mg/L
- Based on:
- other: test material, nominal concentration
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- A mixture containing the test substance, mineral medium and a relative small number of micro¬organism were kept in completely full and closed bottles in dark at constant temperature.
There was a weekly (daily) analysis of dissolved oxygen for the expression of biodegradation as percentage of ThOD.
Dilution water: deionized (distillate) water.
Mineral medium: As described in the guideline (OECD 301 D).
Procedure:
a) Preparation of bottles
The preparation of the stock solution was set up by a weighted portion of about 40 mg from the test substance which was made up to 100 ml with distillate water (stock solution).
The mineral medium was strongiy aerated for at least 30 minutes and was allowed to stand for 20 h at the test temperature (O2-concentration was about 9 mg/L at 20 °C).
Then there was added about 1.5 I fully-aerated mineral medium to a large bottle (4 I) so that it was about one-third full. Afterwards there was added an appropriate amount (about 40 ml) of the stock solution of the test substance to reach the final concentration of 4 mg/L (between 2 and 5). Then the solution was inoculated with secondary effluent. Finally, the solution was made up to volume with aerated mineral medium.
The same procedure was repeated to prepare the reference compound.
Each test series consisted of:
- about 10 bottles containing test suspension (test substance and inoculum)
- about 10 bottles containing inoculum alone (blank inoculum)
- about 10 bottles containing reference compound and inoculum (procedure control)
b) Test procedure
Afterwards the prepared solution was dispensed immediately into the BOD bottles by hose from the lower quarter of the appropriate large bottles, so that all BOD bottles were completely filled. After analysing the zero-time bottles for dissolved oxygen there was a incubating of the other ones in dark and at constant temperature (about 20 °C).
To allow the assessment of percentage removal in a 14 d (10 d) window there was a observation of dissolved oxygen weekly (3 - 4 daily).
All test series were running in parallel.
The reference substance was Propylenalkylbenzolsulfonate with a known biologicat degradation rate (about 30 %).
Reference substance
- Reference substance:
- other: Propylenealkylbenzolsulfonate
Results and discussion
- Preliminary study:
- not applicable
- Test performance:
- For the flasks with the inoculated test substance Nitrate had been determined and the respective amount of oxygen used for nitrification subtracted from the absolute oxygen depletion.
Some validity criteria are not fulfilled:
1) O2-consumption of the inoculum control from day 0 to day 28 was 1.68 mg/L, i.e. > 1.5 mg/L which should not have been exceeded.
2) No replicates in the study; therefore the difference of extremes of replicate values of the removal of the test subst. at day 28 had not been determined (should have been below 20%).
3) The percentage degradation of the reference compound should have reached the pass levels at day 14 (i.e. minimal 60% degradation within 10 days). As the reference substance used does not belong to the ones cited in the guideline, this point can not be checked. The study report states a 30% degradation for the used reference substance within the test, which had been reached, indeed.
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 54
- Sampling time:
- 28 d
- Remarks on result:
- other: In a 10 day window, about 38% had been degraded
- Details on results:
- The ThOD for the test item is given in the study without explanation of the deduction: 1.57 mg O2/mg.
The O2-concentration for all bottles at t=0 is given as about 9 mg/L.
The O2-consumption of the inoculum control from day 0 to day 28 was 1.68 mg/L. A validity criterium of the guideline is therefore not fulfilled (1.5 mg/L should not be exeeded).
A plateau is reached at day 28 at a degradation level of 54%.
BOD5 / COD results
- Results with reference substance:
- The ThOD for the reference substance propylenalkylbenzolsulfonate is given in the study without explanation of the deduction: 2.84 mg O2/mg.
The known degradation level for the reference substance is stated to be about 30%.
The O2-concentration for all bottles at t=0 is given as about 9 mg/L.
The O2-consumption of the inoculum control from day 0 to day 28 was 1.68 mg/L. A validity criterium of the guideline is therefore not fulfilled (1.5 mg/L should not be exeeded).
A plateau is reached at day 28 at a degradation level of about 32%.
Any other information on results incl. tables
biological degradation (BOD/ThOD*100%)
days |
BOD test subst. |
BOD reference subst. |
Biol. degrad. test subst. [%] |
Biol. degrad. reference subst. [%] |
1 |
0.032 |
0.047 |
2.1 |
1.6 |
2 |
0.113 |
0.157 |
7.2 |
5.5 |
5 |
0.240 |
0.384 |
15.3 |
13.5 |
12 |
0.533 |
0.717 |
34.0 |
25.3 |
17 |
0.743 |
0.857 |
47.4 |
30.2 |
23 |
0.825 |
0.892 |
52.7 |
31.4 |
28 |
0.844 |
0.917 |
53.9 |
32.3 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Remarks:
- See section "test performance", as text is truncated here!
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- In a test for ready biodegradability according to OECD 301 D (closed bottle) compliant to GLP (reliability category 2), the test item proved to be not readiliy biodegradable.
Neither the pass level of 60% ThOD (theoretical oxiygen demand), nor the 10-day window had been fulfilled. However, at the end of the test at day 28 a biodegradation of 54 % ThOD had been reached. The result is plausible as degradation proceeded steadily in a linear manner and from day 17 to day 28 approaching a plateau.
According to the ECHA Guidance on Information Requirements, part R.7B, R.7.9.4, for mixtures of homologous compounds the 10 day-window does not apply. Furthermore, results of ready biodegradability tests almost fulfilling the pass level criterion (60% for ThOD) can be used to prove inherent biodegradability, eliminating the need of furthern inherent tests.
Thus, the substance can be regarded as inherently biodegradable. However, as the deduction of the ThOD is not documented in the report, there are some doubts regarding the reliability of this crucial value for determiniation of biodegradation. - Executive summary:
In a test for ready biodegradability according to OECD 301 D (closed bottle), the test item proved to be not readiliy biodegradable.
Neither the pass level of 60% ThOD (theoretical oxiygen demand), nor the 10-day window had been fulfilled. However, at the end of the test at day 28 a biodegradation of 54 % ThOD had been reached. The result is plausible as degradation proceeded steadily in a linear manner and from day 17 to day 28 approaching a plateau.
According to the ECHA Guidance on Information Requirements, part R.7B, R.7.9.4, for mixtures of homologous compounds the 10 day-window does not apply. Furthermore, results of ready biodegradability tests almost fulfilling the pass level criterion (60% for ThOD) can be used to prove inherent biodegradability, eliminating the need of furthern inherent tests.
Thus, the substance can be regarded as inherently biodegradable. However, as the deduction of the ThOD is not documented in the report, there are some doubts regarding the reliability of this crucial value for determiniation of biodegradation.
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