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EC number: 258-649-2 | CAS number: 53585-53-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Sediment toxicity
Administrative data
Link to relevant study record(s)
- Endpoint:
- sediment toxicity: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study based on draft OSPAR protocol, GLP compliant
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- ASTM E1367 (10-day Static Sediment Toxicity Test (Media: Sediment-marine))
- Version / remarks:
- 92
- Principles of method if other than guideline:
- Paris Commission (PARCOM) - Ring Test Protocol. A Sediment Bioassay Using an Amphipod Corophium sp (1993).
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- The concentration of radioactivity in 4 subsamples (ca 0.1 g) of the sediment pre-mix was determined by combustion and liquid scintillation counting. The concentrations in the test vessels were calculated on a dry weight basis from the measured concentrations of radioactivity in the pre-mix and the weights of sediment used.
- Details on sediment and application:
- Sediment was collected from the same location as the Corophium and sieved to 500 µm using natural seawater.
A sediment pre-mix was prepared by adding 45.4 µg [14C]-DBT and ca 210 mg DBT, in acetone to ca 42 g of dry sediment. The acetone was removed under a stream of gaseous nitrogen. This prepared a nominal pre-mix concentration of ca 5000 mg [14C]-DBT.kg-1 sediment (oven dry basis).
Weighed amounts of wet sediment (ca 300 g in the range finding test, ca 900 g in the definitive test) were added to mixing containers, one at each concentration and a weighed amount of dry sediment pre-mix added to the centre of the sediment. Seawater
(100 ml.300 g-1 sediment) was added to each mixing container and the containers placed on a platform shaker at 150 rpm for ca 3 h. The mixed sediment from each container was then added to one test beaker (range finding test), or divided equally between 3 replicate test beakers (main test), per concentration and the sediment left to settle for ca 16 h. Alter the 16 h settling period, seawater was added to each beaker (ca 600 ml) to give a final volume of ça 850 ml and the contents allowed to settle for a further ca 24 h. Aeration was applied to the beakers for ca 24 h prior to the addition of Corophium.
The control sediment was prepared in the same manner as the test sediment. No sediment pre-mix was added to the wet sediment in the mixing container.
The concentration of [14C]-DBT in each test vessel was calculated from measurements of the concentration of total radioactivity in the sediment pre-mix. - Test organisms (species):
- Corophium volutator
- Details on test organisms:
- Corophium volutator of length 5-8 mm were used for these studies, and were obtained from the Orkney Water Test Centre, Orkney on 16 January 1997. They were maintained in natural seawater collected from a site on the east coast of Scotland.
All Corophium were acclimatised to laboratory conditions for at least 5 days prior to commencement of the study. Only healthy, undamaged animais were used for the test. - Study type:
- laboratory study
- Test type:
- static
- Water media type:
- saltwater
- Type of sediment:
- natural sediment
- Limit test:
- no
- Duration:
- 10 d
- Exposure phase:
- total exposure duration
- Test temperature:
- 15+/-1°C
- pH:
- 8.0 +/- 0.1
- Dissolved oxygen:
- > 90%
- Salinity:
- 34 %o
- Nominal and measured concentrations:
- Measured concentrations: see table below
- Key result
- Duration:
- 10 d
- Dose descriptor:
- LC50
- Effect conc.:
- 119.3 mg/kg sediment dw
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 10 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 45.1 mg/kg sediment dw
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- Mortalities Recorded During the Definitive Test
(Sixty Animais per Concentration)
Time Measured Concentration of Test Material (mg.kg-1) -
0 11.2 23.0 45.1 89.0 179.5
10d 5* 3* 3+5* 3* 4+19* 6+37*
* = Not found in sediment - Validity criteria fulfilled:
- not specified
- Conclusions:
- LC50-10d = 119.3 mg/kg dw, NOEC = 45.1 mg/kg dw
- Executive summary:
Acute toxicity to Corophium volutator was carried out according to PARCOM ring test protocol (1993), sediment was spiked by 14C-dibenzyltoluene. Assessment of mortality after 10 days exposure was made and LC50 and NOEC derived.
- Endpoint:
- sediment toxicity: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: study done according to ASTM standard, GLP compliant
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM E1367-90, Standard Guide For Conducting 10 day Static Sediment Toxicity Tests with Marine and Estuarine Sediments.
- GLP compliance:
- yes
- Remarks:
- accredited by Norwegian Authority
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on sediment and application:
- PREPARATION OF SPIKED SEDIMENT
- Sediment collected at an unpolluted site was used for preparing test sediments. The sediment is a muddy sand and was analysed for organic content and proportion silt for each test.
- Each concentration was prepared by dissolving the product in 20 ml of acetone, this solution was added to 100 g pre-dried sediment. After the acetone had dried off in a fume hood, the sample was added to 500 g of damp sediment in a plastic bucket. 350 ml of sea-water was added, and the bucket was shaken for 3 hours. The sediment was then divided into three beakers and Ieft for a standstill for at least 2 hours. Then 300 ml of seawater was added to, and 10 animais were randomly selected for each beaker. A sample of the damp sediment was analysed for water content, and ail concentrations are calculated as mg kg-1 sediment dry weight.
- Test organisms (species):
- Corophium volutator
- Details on test organisms:
- Corophium volutator , from an unpolluted site in Bergen.
The test organisms were supplied as aduits (4-8 mm in size) and were held in aerated seawater at 15°C. A small amount of natural seaweed was used for feeding. - Study type:
- laboratory study
- Test type:
- static
- Water media type:
- saltwater
- Type of sediment:
- natural sediment
- Limit test:
- no
- Duration:
- 10 d
- Exposure phase:
- total exposure duration
- Test temperature:
- 15 ± 1°C
- pH:
- From 8.1 (start) to 8.0 (end)
- Dissolved oxygen:
- From 93 (start) to 94 (end) % of saturation.
- Salinity:
- 34 %o
- Nominal and measured concentrations:
- nominal
- Details on test conditions:
- The test was run in glass containers (600 ml) each containing 200 g of test sediment, app. 400 ml of seawater and 10 animais, 3 replicates for each concentration. The beakers were kept under continuous aeration at a temperature of 15 ± 1°C. pH and oxygen measurements were performed as indicated on the data sheet.
- Reference substance (positive control):
- yes
- Remarks:
- Fluoranthene
- Duration:
- 10 d
- Dose descriptor:
- LC50
- Effect conc.:
- 99.9 mg/kg sediment dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL: 85.4 - 120
- Duration:
- 10 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 40.2 mg/kg sediment dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- The effect on the survival of Corophium volutator is expressed as the concentration of a product lethal to 50% of the test animais (LC50). The combined mortality per test concentration were used for caiculation. The 10 day LC50 value and the 95% confidence limit were calculated by using the standard PROBIT Analysis, National Swedish Environmental Protection Board (Version 2.3, 1990). This programme also calculate test concentrations lethal to 10% and 90% of the test animais.
- Results with reference substance (positive control):
- LC50-10d = 17.2 mg/kg dw
- Validity criteria fulfilled:
- yes
- Remarks:
- The validity criteria given in the test protocol are fulfilled, in that the dissolved oxygen concentration at the end of the test was greater than or equal to 60 %, and the percentage mortality in the controls were less than 15%. The sediment used is foun
- Conclusions:
- LC50-10d = 99.9 mg/kg dw, NOEC = 40.2 mg/kg dw
- Executive summary:
Acute toxicity to Corophium volutator was carried out according to ASTM standard, sediment was spiked by dibenzyltoluene. Assessment of mortality after 10 days exposure was made and LC50 and NOEC derived.
Referenceopen allclose all
Description of key information
10-day acute toxicity reliable studies have been conducted on marine sediment reworker.
LC50s are about 110 mg/kg dw and NOECs about 42 mg/kg dw.
Key value for chemical safety assessment
- EC50 or LC50 for marine water sediment:
- 110 mg/kg sediment dw
- EC10, LC10 or NOEC for marine water sediment:
- 42 mg/kg sediment dw
Additional information
2 studies have been carried out on the marine sediment reworker Corophium volutator (mud shrimp), with exposure through sediment.
Both studies give very close results with resp.: LC50-10d = 99.9 mg/kg dw, NOEC = 40.2 mg/kg dw and LC50-10d = 119.3 mg/kg dw, NOEC = 45.1 mg/kg dw.
The average of the 2 studies results was retained to derive the PNEC sediment.
Nevertheless, from information available on production dry process and uses that are in closed systems, recovery of the used product for incineration, no exposure of sediment is expected.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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