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EC number: 224-069-3 | CAS number: 4191-73-5
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- fish early-life stage toxicity
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Justification for type of information:
- Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- Chronic toxicity test was performed for 7 days at different concentrations.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Isopropylparaben
- Molecular formula: C10-H12-O3
- Molecular weight: 180.202 g/mol
- Smiles notation: c1(C(OC(C) C)=O)ccc(O)cc1
- InChl : 1S/C10H12O3/c17( 2)1310(12)8359(11)648/h37,11H,12H3
- Substance type: Organic
- Physical state: Solid - Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: Fathead minnow
- Age at study initiation (mean and range, SD): 24 h posthatch larvae
- Feeding during test: yes
- Food type: newly hatched Artemia nauplii
- Frequency: twice daily - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 7 d
- Hardness:
- 160 to 175 mg/L as CaCO3
- Test temperature:
- 24°C
- pH:
- 8.2 to 8.8
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Conductivity:
- No data
- Nominal and measured concentrations:
- Nominal concentration: Five concentrations
- Details on test conditions:
- TEST SYSTEM
- Material, size, headspace, fill volume: 250 ml
- Aeration: Yes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): four replicates
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: test solution + a RHW and 0.1% acetone solvent control
- Alkalinity: 110 to 120 mg/L as CaCO3
- Conductivity: 535 to 588 mS/cm
OTHER TEST CONDITIONS
- Adjustment of pH: 8.2 to 8.8
- Photoperiod: 16:8 light: dark cycle
TEST CONCENTRATIONS
- Range finding study: Half of the LC50 determined from the acute test was used as the highest subchronic exposure concentration.
- Test concentrations: five concentration was mention but values are not specified (range from 1.0 to 25 mg/L) - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Validity criteria fulfilled:
- not specified
- Conclusions:
- In long term toxicity study the low observed effect (LOEC) concentration for test species Pimephales promelas was observed to be 9.0 mg/L concentrations for Isopropylparaben(4191-73-5).
- Executive summary:
Long term toxicity to fish was performed for 7 days at five different concentrations ranges from 1.0 to 25 mg/L. Four replicates of 10 P. promelas in 250 ml of test solution for each concentration were prepared and renewed daily. Fish were fed twice daily and mortality was observed daily. After 7 d of exposure, average weight per fish was calculated and compared to the control.
Thus, on the basis of above result the LOEC for long term toxicity to P. promelas was observed to be 9.0 mg/L for test chemical Isopropylparaben (4191-73-5)
Reference
Description of key information
In long term toxicity study the low observed effect (LOEC) concentration for test species Pimephales promelas was observed to be 9.0 mg/L concentrations for Isopropylparaben(4191-73-5).
Key value for chemical safety assessment
Additional information
Long term toxicity to fish was performed for 7 days at five different concentrations ranges from 1.0 to 25 mg/L. Four replicates of 10 P. promelas in 250 ml of test solution for each concentration were prepared and renewed daily. Fish were fed twice daily and mortality was observed daily. After 7 d of exposure, average weight per fish was calculated and compared to the control.
Thus, on the basis of above result the LOEC for long term toxicity to P. promelas was observed to be 9.0 mg/L for test chemical Isopropylparaben (4191-73-5)
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