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EC number: 212-606-4 | CAS number: 831-59-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed according to the guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Principles of method if other than guideline:
- Acute Immobilization Study of disodium benzene-1,3-disulphonate (831-59-4) on Daphnia magna in a Semi-Static System for 48 hrs. Test performed according to the OECD guideline 202.
- GLP compliance:
- no
- Analytical monitoring:
- not specified
- Vehicle:
- no
- Details on test solutions:
- The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 2 mg of the test substance in 20 ml of ADaM’s media. Achieving test concentrations of 100 mg/L, respectively.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Common name: Water flea
- Source: Own breeding of daphnia magna
- Food: No feeding after hatching - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- 24 and 48 hrs
- Hardness:
- 153.5 mg of CaCO3
- Test temperature:
- 20±2
- pH:
- 7.1
- Nominal and measured concentrations:
- 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids
OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark
- Reference substance (positive control):
- not specified
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Remarks:
- 1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels.
- Conclusions:
- Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for disodium benzene-1,3-disulphonate (831-59-4) on test daphnia was determined to be > 100 mg/L.
- Executive summary:
Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for disodium benzene-1,3-disulphonate (831-59-4).
The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 2 mg of the test substance in 20 ml of ADaM’s media. Achieving test concentrations of 100 mg/L, respectivelyThe nominal concentration selected for the experiment was 100 mg/l- and test Daphnia magna were exposed to these concentration for 48 hours. The median lethal concentration (EC50) for disodium benzene-1,3-disulphonate (831-59-4) on Daphnia magna in a 48 hours study on the basis of immobilization effect was determined to be > 100 mg/L.Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, disodium benzene-1,3-disulphonate (831-59-4) does not exhibit short term toxicity to Daphnia. EC50 (48 hours) Experimental is > 100 mg/L.After 48 hours of exposure to test item disodium benzene-1,3-disulphonate (831-59-4) to various nominal test concentrations, EC50 was determine to be > 100 mg/l . Based on the EC50, it can be consider that the chemical was non-hazardous and can be consider to be not classified as per the CLP classification criteria.
Reference
Immobility
Nominal Concentration (mg/l) |
Number of Daphnids |
Immobility |
||
0 h |
24h |
48h |
||
Control |
10 |
- |
- |
- |
100 |
10 |
- |
- |
- |
pH and oxygen content:
Nominal Concentration (mg/l) |
Number of Daphnids |
pH(mg/L) |
|
|
0 h |
48h |
|
||
Control |
10 |
7.2 |
7.4 |
|
100 |
10 |
7.3 |
7.5 |
|
Nominal Concentration (mg/l) |
Number of Daphnids |
DO(mg/L) |
|
|
0 h |
48h |
|
||
Control |
10 |
7.1 |
7.0 |
|
100 |
10 |
7.0 |
6.9 |
|
Test temperature:
Nominal Concentration (mg/l) |
Number of Daphnids |
Temparature |
|
|
0 h |
48h |
|
||
Control |
10 |
21.2 |
21.3 |
|
100 |
10 |
21.2 |
21.3 |
|
Description of key information
Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for disodium benzene-1,3-disulphonate (831-59-4) on test daphnia was determined to be > 100 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Short term toxicity to aquatic invertebrates for disodium benzene-1, 3-disulfonate was summarised (831-59-4) with two experimental studies are summaries as follows:
Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for disodium benzene-1,3-disulphonate (831-59-4).
The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 2 mg of the test substance in 20 ml of ADaM’s media. Achieving test concentrations of 100 mg/L, respectivelyThe nominal concentration selected for the experiment was 100 mg/l- and test Daphnia magna were exposed to these concentration for 48 hours. The median lethal concentration (EC50) for disodium benzene-1,3-disulphonate (831-59-4) on Daphnia magna in a 48 hours study on the basis of immobilization effect was determined to be > 100 mg/L.Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, disodium benzene-1,3-disulphonate (831-59-4) does not exhibit short term toxicity to Daphnia. EC50 (48 hours) Experimental is > 100 mg/L.After 48 hours of exposure to test item disodium benzene-1,3-disulphonate (831-59-4) to various nominal test concentrations, EC50 was determine to be > 100 mg/l . Based on the EC50, it can be consider that the chemical was non-hazardous and can be consider to be not classified as per the CLP classification criteria.
Aim of this study was to assess the short term toxicity of disodium benzene-1,3-disulphonate (831-59-4) to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. The solution 100.0 mg/l was prepared by dissolving beige powder in reconstituted water.100 mg/l concentrations was used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously.
The median effective concentration (EC50) for the test substance disodium benzene-1,3-disulphonate (831-59-4), in Daphnia magna,8% of inhibition of mobility was observed at test concentration 100 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the I %, substance is likely to be non hazardous to aquatic invertebrate and cannot be classified as per the CLP classification criteria.
Based on the above data of target, it was considered that disodium benzene-1, 3-disulfonate(CAS: 831-59-4) was not hazardous to aquatic invertebrates and can be considered to be not classified as per the CLP regulations.
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