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EC number: 247-820-7 | CAS number: 26576-46-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989-08-14 to 1989-09-01
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study performed according to OECD Guideline but solvent was used.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- N-(2,3-dihydro-2-oxo-1H-benzimidazol-5-yl)-3-oxobutyramide
- EC Number:
- 247-820-7
- EC Name:
- N-(2,3-dihydro-2-oxo-1H-benzimidazol-5-yl)-3-oxobutyramide
- Cas Number:
- 26576-46-5
- Molecular formula:
- C11H11N3O3
- IUPAC Name:
- 3-oxo-N-(2-oxo-2,3-dihydro-1H-benzimidazol-5-yl)butanamide
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - One aliquot part of 100mL was taken from the total volume of 10L
- Sample was clear
- Water temperature was 22°C +/- 1°C during toxicity testing
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Diliuton water:
- Reconstituted water according to ISO/DIS 7346/1 was used
- Water was ventilated until oxygen saturation was reached
- pH was determined prior to testing and was between 8.2 and 8.3
Test item:
- Test item was wetted with 0.095mL of Tween 80 per litre to facilitate dissolving; then dilution water was added; both was homogenised using ultrasonic bath and ultra turrax
- While stirring with a glas bar the homogenises solution was transferred to the bassin
- The whole volume was stirred for 2hrs using a KPG-stirrer
- The test item was dissolved with minor sedimentation of the test item on the ground of the vessel and at the water surface. 96hrs after exposure a minor blurring was observed
- To determine the actual concentration of dissolved test item water samples were taken at the beginning of the test and after 48hrs and 96hrs. Samples were taken from the middle of the bassin. Analytical determined mean values were above 80%
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- - Origin: West Aquarium, 3422 Bad Lauterberg
- Date of birth: 1989-05-14
- Date of delivery: 1989-08-03
- Fishes were adapted to the water and surrounding conditions for at least 14d prior to exposure
- Water tempereature: 22°C +/- 1°C
- Oxygen concentration: >= 80% of oxygen saturation
- Illumination: 12hrs per day
- Animal density: =< 1g fish per litre water
- Feeding: twice a day, ad libitum
- Feed: Tetra Min, Tetra Werke, Melle
- Prior to testing, 10 fishes were randomly picked to be representatively measured for their body size; these 10 fishes were not used for the experiment
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- 22°C +/-1°C
- pH:
- 8.2 - 8.3
- Dissolved oxygen:
- >= 80%
- Nominal and measured concentrations:
- Nominal: 500mg/L
Measured: 475.3mg/L, equalling 95.1% (506, 431 and 489 mg/l at d0, d2 and d4) - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: none
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: none
- Details on results:
- - No lethality observed at 500mg/L during the whole experiment
- Therefore, LD50 after 48hrs and 96hrs is above 500mg/L
- Fish from the control group (Tween 80) and the 500mg/L test item group exhibited impairment of breathing, altered swimming behaviour and altered general behaviour
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
Testing for lethal effects of Acetolon TTR was done using Brachydanio rerio following the above described Guidelines. Over a period of 96hrs no lethal effects were observed at a concentration of 500 mg/L. Therefore, LC50 for 48hrs and 96hrs is above 500mg/L.
Due to dissolving behaviour of Acetolon TTR in dissolving pre-test, Tween 80 was used as a solubiliser. Possible effects of Tween 80 were assessed in a solvent control, using 0.095mL of Tween 80 per litre.
The test item was dissolved with minor sedimentation of the test item on the ground of the vessel and at the water surface. 96hrs after exposure a minor blurring was observed.
To determine the actual concentration of dissolved test item water samples were taken at the beginning of the test, after 48hrs and 96hrs. Samples were taken from the middle of the bassin. Analytical determined mean values were at this time points always well above 80%.
Since LC50 > 500 mg/L after 96 hours no classification is required
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