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EC number: 941-637-2 | CAS number: 1384257-92-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015-02-03 to 2015-05-04
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- (2004)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentration: 100 mg/L (limit test)
- Sampling method:
- Sample storage conditions before analysis: - Vehicle:
- no
- Details on test solutions:
- Reconstituted water according to ELENDT (1990):
Macro nutrients (mg/L):
CaCI2 x 2 H20 147
MgSO4 x 7 H20 123
NaHCO3 64.8
KCI 5.80
Na2SiO3 4.30
NaNO3 0.27
KH2PO4 0.14
K2HPO4 0.18
Na2EDTA x 2H20 5.00
FeSO4 x 7 H2O 1.99
H3BO3 0.29
MnCl2 x 4 H2O 0.36
LiCl 0.30
SrCl2 x 6 H2O 0.15
RbCl 0.071
NaBr 0.016
Na2MoO4 x 2 H2O 0.063
CuCl x 2 H2O 0.017
ZnCl2 0.013
CoCl2 x 6 H2O 0.010
KJ 0.00325
Na2SeO3 0.00219
NH4VO3 0.000575
Thiaminhydrochloride 0.075
Cyanocobalamine 0.0010
Biotin 0.00075
pH: 8.2 ± 0.8, after an aeration for 24 hours.
References:
ELENDT, B.-P. Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus.
Protoplasma 154, 25-33, 1990
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test item was dissolved in culture medium (ELENDT) at a concentration of 100 mg/L. The solution was stirred (approx. 1100 rpm) for 24 hours at room temperature.
- Controls: Culture medium without test item - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain/clone: clone 5
- Source: Institut für Wasser-, Boden- und Lufthygiene, D-14195 Berlin, Germany
- Feeding during test: No
- Age: not older than 24 hours - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 263 mg CaCO3/L
- Test temperature:
- 18-22°C
- pH:
- 7.54 - 7.75
- Dissolved oxygen:
- 7.96 - 8.57 mg/L
- Conductivity:
- 653 µS/cm
- Nominal and measured concentrations:
- Nominal concentration: 100 mg/L (limit test)
Measured concentrations:
Start of exposure (0 h): 104 mg/L
End of exposure (48 h): 98.1 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers, 50 mL capacity
- Type (delete if not applicable): open (loosely covered with watch glasses)
- Material, size, headspace, fill volume: glass, 4 (ID) x 7 (H) cm), 20 mL fill volume
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO test water, according to OECD 202, Annex 3
- Intervals of water quality measurement:
At the start of exposure (0 h) and end of exposure (48 h) : pH value, dissolved oxygen, temperature, conductivity and total hardness
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8 h light/dark cycle
- Light intensity: Diffuse light, light intensity of max. 20 µE x m-2 x s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Immobilisation was determined in all groups after 24 and 48 h.
VEHICLE CONTROL PERFORMED: not applicable
RANGE-FINDING STUDY
- Test concentrations: 1.00, 10.0 and 100 mg/L
- Results used to determine the conditions for the definitive study:
No significant effects were observed at any of the tested concentration levels. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Clinical findings
The nominal concentration of the preparation of the test substance and the immobilization data are shown in the following table:
--------------------------------------------------------------------
Nominal concentration
[mg/L] Immobilized/exposed Daphnia magna
---------------------------
24 hours 48 hours
--------------------------------------------------------------------
0 0/20 0/20
100 0/20 0/20
--------------------------------------------------------------------
A dose group with nominal 100 mg/L of the test item revealed no aquatic toxicity in this test system.
No remarkable observations were made concerning the appearance of the solution of the test material preparation. The test medium was a clear preparation and stayed unchanged throughout the study.
EC50 value
An aqueous solution of nominal 100 mg/L of the test substance revealed no aquatic toxicity in the test system. The 24 hours and 48 hours EC50 to Daphnia magna exceeded the limit dose of 100 mg/L of the test item and, thus, was not determined in this test.
Analysis
pH, oxygen concentrations, and temperature during the study were inconspicuous.
--------------------------------------------------------------------------------------------------------------------------
Nominal concentration
[mg/L] Start of the experiment End of the experiment
---------------------------
0 hour 48 hours (mean)
pH O2 [mg/L] pH O2 [%]
-----------------------------------------------------------------------------------------------------------------------------
0 7.75 8.57 7.58 8.48
100 7.56 7.96 7.54 8.39
------------------------------------------------------------------------------------------------------------------------------ - Results with reference substance (positive control):
- The percentage immobility for the reference item was determined after 24 h. The EC50-value with 95% confidence limits (CI) was calculated by sigmoidal dose-response regression. The EC50-value for the most recent of the monthly performed reference tests was: EC50 = 2.01 mg/L (CI: 1.71 - 2.26 mg/L). The EC50-value of the refernce item potassium dichromate after 24 h is within the prescribed concentration range of 0.6 - 2.4 mg/L of quality criteria according to AQS P 9/2 (02/2000) for daphnids clone 5 cultured in Elendt M4 medium. The EC50-value of the refence item is also within the the recommended range of 0.6 -2.1 mg/L according to OECD-Guideline 202.
- Validity criteria fulfilled:
- yes
- Conclusions:
- At the analytically confirmed nominal concentration of 100 mg/L of the test item, no effects were observed in Daphnia magna.
- Executive summary:
see above
Reference
In the acute immobilisation test with Daphnia magna (STRAUS), the effects of a limit concentration of 100 mg/L of the test item were determined according to OECD 202 (2004).
The limit test was conducted under static conditions over a period of 48 h. The test item is a colourless liquid. The limit concentration was stirred with approximately 1100 rpm for 24 hours at room temperature before the start of the exposure and was clear throughout the exposure period.
Twenty daphnids each were exposed to the limit concentration and the control.
The concentrations of the test item were analytically verified via LC-MS/MS at the start of the exposure (0 h) and at the end of the exposure (48 h) in the limit concentration and the control. Details of the analytical method are presented in the report.
The measured concentration of the test item at the start of the exposure (0 h) was 104 mg/L. At the end of the exposure (48 h), the measured concentration of the test item was 98.1 mg/L. The measured test item concentration was within ± 20 % of the nominal concentration.
This indicates that the test item concentration was successfully maintained for the duration of the test. The effect levels given are based on the nominal concentration of the test item.
The water quality parameters (i.e. pH-value and dissolved oxygen concentration), measured at the start (0 h) and at the end of the exposure (48 h), were within the acceptable limits. The validity criteria of the test guideline were fulfilled.
Effect levels:
EC50 (24 h): > 100 mg/L
EC50 (48 h): > 100 mg/L
NOEC (48 h): 100 mg/L
LOEC (48 h) > 100 mg/L
Description of key information
In an acute immobilisation test with Daphnia magna (STRAUS), the effects of a limit concentration of 100 mg/L of the test item were determined according to OECD 202 (2004).
The limit test was conducted under static conditions over a period of 48 h. The test item is a colourless liquid. The limit concentration was stirred with approximately 1100 rpm for 24 hours at room temperature before the start of the exposure and was clear throughout the exposure period.
Twenty daphnids each were exposed to the limit concentration and the control.
The concentrations of the test item were analytically verified via LC-MS/MS at the start of the exposure (0 h) and at the end of the exposure (48 h) in the limit concentration and the control. Details of the analytical method are presented in the report.
The measured concentration of the test item at the start of the exposure (0 h) was 104 mg/L. At the end of the exposure (48 h), the measured concentration of the test item was 98.1 mg/L. The measured test item concentration was within ± 20 % of the nominal concentration.
This indicates that the test item concentration was successfully maintained for the duration of the test. The effect levels given are based on the nominal concentration of the test item.
The water quality parameters (i.e. pH-value and dissolved oxygen concentration), measured at the start (0 h) and at the end of the exposure (48 h), were within the acceptable limits. The validity criteria of the test guideline were fulfilled.
Effect levels:
EC50 (24 h): > 100 mg/L
EC50 (48 h): > 100 mg/L
NOEC (48 h): 100 mg/L
LOEC (48 h) > 100 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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