Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 800-036-4 | CAS number: 1422423-64-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From March 16th to 18th, 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- adopted 22nd July 2010
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- no analyses of the test concentrations were conducted.
- Details on test solutions:
- Since the test item is well soluble (325-350 g/l), the test concentrations were prepared by dilution, with natural water, of a stock solution prepared in natural water.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source: non-adapted and not pre-conditioned activated sludge from the aeration tank of the ARA Werdhölzli (CH-8048 Zürich), a municipal biological waste water treatment plant.
- Sampling: 14th March 2016; 11.00 a.m.
- Preparation of inoculum for exposure: prior to the tests, the sludge was washed with natural water, and finally suspended in natural water at a concentration twice of the final concentration.
- Pretreatment: to keep the viability of the sludge, 80 ml of the synthetic sewage feed were added per litre of activated sludge (the volume taken into account is the volume of settled suspension) and the sludge was aerated overnight.
- Initial biomass concentration: 1.5 g/l dry matter in the final mixture. - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 3 h
- Test temperature:
- 22 ± 2 °C
- pH:
- All values were within an acceptable range.
- Nominal and measured concentrations:
- Nominal concentrations: 31.6, 100, 316 and 1000 mg/l.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1000 ml glass flasks, containing a total volume of test suspension of 516 ml.
- Aeration: after addition of the activated sludge suspension, the test vessels were aerated by shaking (150 r.p.m.) for the whole test period of 3 hours.
- No. of vessels per concentration: activated sludge, test medium and test item, three replicates per test concentration.
- No. of vessels per blank control: activated sludge and test medium, six replicates.
- No. of vessels per sensitive control: activated sludge, test medium and 3,5-dichlorophenol as reference item (3, 10 and 30 mg/l), one vessel per test concentration.
- Sludge concentration: 3 g/l dry matter in the final mixture. 250 ml of the double-concentrated activated sludge suspension were added to each test vessel.
SYNTHETIC SEWAGE FEED
- Source/preparation: synthetic sewage feed prepared with ultra-pure water, as 100-fold sewage concentrate. 32 ml/l in the final mixture.
- Composition of synthetic sewage feed (dissolved amount per litre of ultra-pure water):
Peptone: 16 g
Meat extract: 11 g
Urea: 3 g
Sodium chloride: 0.7 g
Calcium chloride dihydrate: 0.4 g
Magnesium sulfate heptahydrate: 0.2 g
Dipotassium hydrogen phosphate: 2.8 g
- pH: the initial pH-value is 7.5 ± 0.5.
- Amount of synthetic sewage: 16 ml of 100-fold concentrated synthetic sewage were added to each test vessel.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural water supplying the laboratory.
- pH: 7.4 - 7.8
- Electric conductivity (room temperature): 477 - 536 μS/cm
- Total Hardness: 20.6 - 24.2 °f H; 2.06 - 2.42 mmol/l; 206 - 242 mg/l
- CO3 Hardness: 18.6 - 21.7 °f H; 186 - 217 mg/l
- Residual Hardness: 2.0 - 3.0 °f H; 20 - 30 mg/l
- Acid consumption: 3.72 - 4.34 mmol/l
- Colony counts (30°C): 0 - 1 per ml
- Escherichia coli: 0 per 100 ml
- Enterococcus: 0 per 100 ml
- Nitrate: 7.3 mg/l
- Chloride: 16.1 mg/l
- Sulfate: 18.6 mg/l
EFFECT PARAMETERS MEASURED
- Measuring apparatus: 300 ml flat bottom BOD flasks; polarographic oxygen electrode, connected to a potentiometric recorder.
- Sampling time: the respiration rate in each sample was measured 3 h after addition of the activated sludge suspension (following the starting sequence as described above), at 5–15 min intervals.
- Oxygen consumption: the respiration rate, R (in mg O2/l/h) is calculated from the linear part of the graphs of oxygen decrease versus time. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (tested at 3, 10 and 30 mg/l)
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- No significant inhibition of the activated sludge respiration, as compared to the untreated controls, was observed at any of the four concentrations tested.
Based on the data recorded, the median effect concentration EC50 of test item was estimated to be higher than 1000 mg/l nominal concentration.
The EC10, EC20, and EC80 values were also estimated to be higher than 1000 mg/l nominal concentration.
The no observed effect concentration (NOEC) was 1000 mg/l nominal concentration, as determined by Dunnett’s test.
VALIDITY OF THE TEST
All validity criteria were fulfilled.
- The oxygen uptake rate in the blank controls was 34.4 mg O2 per gram of activated sludge per hour (required >20 mg O2 per gram dry matter sludge per hour).
- The coefficient of variation of the oxygen uptake rate in the blank controls replicates was 5.7 % (required ≤ 30 %).
- The EC50 value of the reference item 3,5-dichlorophenol was estimated to be 6.4 mg/l (required 2–25 mg/l). - Results with reference substance (positive control):
- The EC50 value of the reference item 3,5-dichlorophenol was estimated to be 6.4 mg/l (required 2 - 25 mg/l).
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 (3h) > 1000 mg/l nominal concentration.
EC10, EC20, and EC80 (3h) > 1000 mg/l nominal concentration
NOEC (3h): 1000 mg/l nominal concentration, as determined by Dunnett’s test. - Executive summary:
Method
The inhibitory effect of test item on the oxygen consumption of activated sludge microorganisms of a municipal sewage treatment plant was investigated according to the OECD Guideline 209 under aerobic static conditions over an exposure time of 3 hours. The inhibitory effect may include the effect on respiration and nitrification.
The nominal concentrations tested were 31.6, 100, 316 and 1000 mg/l. Three parallel test vessels were used for each concentration of the test item and six vessels for the blank controls. No chemical analysis of test concentrations was conducted. For the calculation of the effective concentrations the nominal concentrations of the test item were used, assuming the test item to be stable in water over 3 h.
Results
No significant inhibition of the activated sludge respiration, as compared to the untreated controls, was observed at any of the four concentrations tested. Based on these data, the median effect concentration EC50 of test item was estimated to be > 1000 mg/l nominal concentration.
The EC10, EC20, and EC80, values of tested substance were also estimated to be > 1000 mg/l nominal concentration.
The no observed effect concentration (NOEC) was equal/higher than 1000 mg/l nominal concentration, as determined by Dunnett’s test.
Reference
Activated sludge respiration rates after 3 h of exposure to the test and reference items and calculation of the inhibition
Nominal Concentration (mg/l) | Sample | O2 consumption rates (mg O2/l/h) |
Respiration inhibition (%) | Respiration inhibition (%), Mean value |
0 | Blank | 58.3 | - | - |
Blank | 51.5 | - | ||
Blank | 52.0 | - | ||
Blank | 49.5 | - | ||
Blank | 54.0 | - | ||
Blank | 52.5 | - | ||
3 | 3,5-dichlorophenol | 37.4 | 29.4 | 29.4 |
10 | 3,5-dichlorophenol | 19.0 | 64.1 | 64.1 |
30 | 3,5-dichlorophenol | 6.4 | 87.9 | 87.9 |
31.6 | Test item | 48.9 | 7.6 | 2.6 |
Test item | 50.2 | 5.3 | ||
Test item | 55.7 | -5.2 | ||
100 | Test item | 51.5 | 2.8 | 1.4 |
Test item | 52.4 | 1.2 | ||
Test item | 52.8 | 0.3 | ||
316 | Test item | 54.7 | -3.3 | 1.3 |
Test item | 51.6 | 2.5 | ||
Test item | 50.5 | 4.7 | ||
1000 | Test item | 48.9 | 7.6 | 0.5 |
Test item | 53.7 | -1.4 | ||
Test item | 55.4 | -4.7 |
Description of key information
EC50 (3h) > 1000 mg/l (nominal)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
The inhibitory effect of test item on the oxygen consumption of activated sludge microorganisms of a municipal sewage treatment plant was investigated according to the OECD Guideline 209 under aerobic static conditions over an exposure time of 3 hours. The inhibitory effect may include the effect on respiration and nitrification. The nominal concentrations tested were 31.6, 100, 316 and 1000 mg/l.
No significant inhibition of the activated sludge respiration, as compared to the untreated controls, was observed at any of the four concentrations tested. Based on the study outcomes, the median effect concentration EC50 of test item was estimated to be higher than 1000 mg/l nominal concentration.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.