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EC number: 206-496-7 | CAS number: 350-03-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 18 Octobre - 05 November 2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well-documented GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Methyl 3-pyridyl ketone
- EC Number:
- 206-496-7
- EC Name:
- Methyl 3-pyridyl ketone
- Cas Number:
- 350-03-8
- Molecular formula:
- C7H7NO
- IUPAC Name:
- 1-(pyridin-3-yl)ethan-1-one
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report): 3-Acetylpyridine
- Physical state: transparent liquid
- Storage condition of test material: keep cool and dry, 15-25°C, avoid light, product is hygroscopic
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable.
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- In the beginning and the end of total test the determination of test substance concentration was performed by means of HPLC in solutions with the highest and the lowest test substance concentration. The solutions before addition of fish (as control of nominal concentration) and the solutions containing fish immediately after its addition were analysed at the beginning of test. The test solutions with test organisms were analysed at the end of test.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Whereas the test substance is soluble in water the basic solution was prepared in dilution water. The concentrations of solutions used in the preliminary and total test were obtained by diluting the basic solution with dilution water.
Preliminary test :
The test solutions were prepared by dosing of appropriate volumes of basic solution into a 1000 mL volumetric flasks and replenishment with dilution water to mark. Content of each volumetric flask was transferred into a 2 L flask.
Dilution water was prepared from distilled water with conductivity of 0.167 mS/m.
-pH of dilution water : 7.79
-Oxygen content : 8.7 mg/L at temperature of 21.1 °C.
-Basic solution of test substance : 100.0 mg/L in dilution water
-Basic solution dosing : 1000, 600, 200, 100, 75, 50 and 25 mL in 1000 mL of dilution water
On the basis of preliminary test results the total test was performed.
Total test :
The total test was carried out at 7 concentrations of the test substance concurrently with control test (without addition of test substance). The total volume each of testing mixtures and control was 4000 mL and 10 pieces of fish were used.
Dilution water was prepared from distilled water with conductivity of 0.248 mS/M.
-pH of dilution water : 7.91
-Oxygen content : 8.4 mg/L at temperature of 21.1°C
-Content of (Ca + Mg) ions : 2.66 mmol/L
-Basic solution of test substance : 100.0 mg/L in dilution water
-Basic solution dosing : 220, 200, 180, 160, 140, 120, and 100 mL in 4000 mL of dilution water
Test organisms
- Test organisms (species):
- Poecilia reticulata
- Details on test organisms:
- TEST ORGANISM
- Common name: Guppy
- Source: BioTest s.r.o., Konärovice (Czech Republic)
- Date of delivery : 29.09.2004
- Age at study initiation: 3-4 months
- Length at study initiation: 2.0 - 3.0 cm
- Feeding during test: no
ACCLIMATION
- Acclimation period: Fish were acclimatized for 7 days in dilution water; they were not fed during 72 hours before the test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No details are reported.
Test conditions
- Hardness:
- Total test : Content of (Ca + Mg) ions: 2.66 mmol/L
- Test temperature:
- 22±2°C, with a variation of ± 1°C at each individual test.
- pH:
- 7.58 - 7.91
- Dissolved oxygen:
- 7.0 - 8.7 mg/L
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations at total test : 2.5, 3.0, 3.5, 4.0, 4.5, 5.0 and 5.5 mg/L
Measured concentrations at start of test (without organisms) : 2.425 mg/L and 5.360 mg/L
Measured concentrations at start of test (with organisms) : 2.428 mg/L and 5.302 mg/L
Measured concentrations at end of test (with organisms) : 2.392 mg/L and 5.183 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Preliminary test : 2 L flask containing 1000 mL test solution ; Total test : flask (size not stated) containing 4000 mL test solution
- Aeration: no
- Renewal rate of test solution : The solutions were not renewed during 96 hours.
- No. of organisms per vessel: 3 fish for the preliminary test ; 10 fish for the total test
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Standard dilution water was prepared just before the test by admixing 2.5 mL stored solutions A, B, C and D in 1000 mL of distilled water.
A = 117.6 g CaCl3x2H20 in 1L distilled water
B = 49.3 g MgSO4x7H20 in 1L distilled water
C = 25.9 g NaHCO3 in 1L distilled water
D = 2.3 g KCl in 1L distilled water
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Lighting : daylight - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3.97 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL : 3.33-4.62 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 5.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- The calculation of LC50 was performed by means of software „Toxicity" (product of Water Research Institute, Ostrava, Czech Republic). The nominal concentrations at the beginning of the test without organisms as input ones were used.
- Results with reference substance (positive control):
- The sensitivity of the test species and correctness of test performance is periodically verified in three-month period by testing the reference substance,potassium dichromate.
The results of verification test with K2Cr2O7, carried out in period from 18.10. to 22.10.2004, are the following:
48 h - LC50 = 129 mg/L (confidential limit: 99 -158 mg/L)
96 h - LC50 = 111 mg/L (confidential limit: 100-123 mg/L)
The inhibition of test organisms in the reference substance solution meets criteria determined by the interlaboratory tests. The results of the last interlaboratory test arranged by Centre for Assessment of Laboratories of T.G.Masaryk Water Research Institute Prague in 2003 for potassium dichromate were: 96 h - LC50 = 94.9 - 155 mg/L . - Reported statistics and error estimates:
- No details are reported.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Table 1: Fish mortality - preliminary test (18.10. to 22.10.2004)
Concentration (mg/L) 100 60 20 10 7.5 5.0 2.5 C Number of fish 48 hours 0 0 0 0 0 1 3 3 Mortality % 48 hours 100 100 100 100 100 66.6 0 0 Number of fish 96 hours 0 0 0 0 0 0 3 3 Mortality % 96 hours 100 100 100 100 100 100 0 0 Table 2: Fish mortality - total test (01.11. to 05.11.2004)
Nominal concentration mg/L 5.5 5.0 4.5 4.0 3.5 3.0 2.5 C Measured concentration mg/L 5.4 2.4 C Number of fish 24 hours 10 10 10 10 10 10 10 9 Mortality % 24 hours 0 0 0 0 0 0 0 10 Number of fish 48 hours 10 10 10 10 10 10 10 9 Mortality % 48 hours 0 0 0 0 0 0 0 10 Number of fish 72 hours 6 8 8 10 9 10 10 9 Mortality % 72 hours 40 20 20 0 10 0 0 10 Number of fish 96 hours 0 1 2 5 8 10 10 9 Mortality % 96 hours 100 90 80 52 20 0 0 10 Measured concentration is rounded off to one decimal place.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Well-documented GLP guideline study
- Conclusions:
- The study was conducted under GLP according to OECD guideline 203 on the registered substance itself. The method is to be considered scientifically reasonable with no deficiencies in documentation or any deviations, the validity criteria are fulfilled. Hence, the results can be considered as reliable to assess the toxicity of 3-Acetylpyridine to fish.
The study is regarded as a valid guideline study with GLP compliance. According to the reported LC50 value (3.97 mg/L) the test substance 3-Acetylpyridine does not need to be classified as hazardous to aquatic environment. - Executive summary:
The test substance, 3-Acetylpyridine, was tested for acute toxicity on fish Poecilia reticulata.The test was performed according to EU method C.1. - Acute toxicity for fish, which is analogous to OECD Test Guideline No. 203. in a static freshwater test. The study was conducted under GLP compliance. The test duration was 96 hours with substance concentrations of 2.5, 3.0, 3.5, 4.0, 4.5, 5.0 and 5.5 mg/L. A temperature of 21 °C was held constant, the pH value was 7.7 - 7.8 at the beginning of the experiment. A content of (Ca + Mg) ions of 2.66 mmlol/L was determined. During the test the pH values ranged from 7.6 to 7.8 and the oxygen content was measured in a range of 7.0 - 8.7 mg/L . The fish mortality was detected each 24 hours throughout the test.
Test results:
96h-LC0 = 3.0 mg/L
96h-LC100 = 5.5 mg/L
96h-LC50 = 3.97 mg/L
3-Acetylpyridine does not need to be classified as hazardous to the aquatic environment. This is based on an LC50 (96h) of 3.97 mg/L and the fact that the substance is very soluble in water, readily biodegradable, has a BCF <<500 and a log Kow <<4.
For a substance, for which adequate chronic toxicity data are not available, with an 96 hr LC50 (for fish) between > 1 to ≤ 10 mg/L, classification as Aquatic Chronic Category 2 according to Regulation (EC) No 1272/2008 would only be necessary, if the substance at the same time is not rapidly degradable and/or the experimentally determined BCF ≥ 500 (or, if absent, the log Kow ≥ 4); this is not the case for 3 -Acetylpyridine.
There is no indication given that the results from this study are not reliable, the data requirements according to REACh Annex VII are met and no need for further testing was identified.
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