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Diss Factsheets
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EC number: 224-748-4 | CAS number: 4474-24-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- other: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- July 12, 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 976
- Report date:
- 1976
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- not specified
- GLP compliance:
- no
- Test type:
- standard acute method
Test material
- Reference substance name:
- Acid Blue 080 - Similar Substance 02
- IUPAC Name:
- Acid Blue 080 - Similar Substance 02
- Test material form:
- not specified
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Tif: RAIf
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 80 to 110 grams.
- Housing: During the treatment and observation period the animals were housed in groups of 5 in Macrolon cages1 (type 3) .
- Diet: standard diet of pellets - No. 890, Nafag Gossau SG, ad libitum
- Water: ad libitum
- Fasting period before study: Animals fasted overnight.
- Acclimation period: 5 days.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 1 °C
- Humidity (%): 55 ± 5%
- Photoperiod (hrs dark / hrs light): 14 hours light cycle day.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: sesame oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 10, 20, 25, and 30 % - Doses:
- 1000, 3000, 8000, 10000, 15000 mg/kg
- No. of animals per sex per dose:
- 5 x sex x doses
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: daily
- Necropsy of survivors performed: yes - Statistics:
- LD50 including 95% confidence limits was calculated by the probit analysis method (Goulden' A. , Methods of Statistical Analysis, John Wiley and Sons, 1960, 3rd printing, pages 404-408).
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 8 850 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- ca. 7 960 - ca. 9 900
- Remarks on result:
- other: 95% confidence limits
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 5 664 mg/kg bw
- Based on:
- act. ingr.
- Mortality:
- yes, for concentrations above 3000 mg/kg
- Clinical signs:
- other: diarrhea, ditto, reduction in spontaneous motility, ataxia, ventricumbency, muscular hypotonia, hypoventilation. Cyanosis for highest concentration (15000 mg/kg)
Applicant's summary and conclusion
- Interpretation of results:
- other: CLP criteria not met
- Conclusions:
- LD50 = 8850 mg/kg bw (5664 mg/kg bw based on active ingtredient)
- Executive summary:
Method
The substance has been tested for acute toxicity by oral route. Rats of both sexes were tested at concetration of 1000, 3000, 8000, 10000, 15000 mg/kg by by oral intubation. Physical condition and rate of death were monitored throughout the whole observation period.
Conclusion
The compound has practically no acute toxicity to the rat by this route of administration.
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