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EC number: 202-544-6 | CAS number: 96-91-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from Water Resources Bulletin
Data source
Reference
- Reference Type:
- publication
- Title:
- Acute Toxicity of Picric Acid and Picramic Acid to Rainbow Trout, Salmo Gairdneri, and American Oyster, Crassostrea Virginica’
- Author:
- William L. Goodfellow, Jr., Dennis T. Burton, William C. Graves, Lenwood W. Hall, Jr., and Keith R. Cooper
- Year:
- 1 983
- Bibliographic source:
- Water Resources Bulletin, VOL. 19, NO. 4-641-648, AUGUST 1983
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as below
- Principles of method if other than guideline:
- Standard Methods (APHA, et al., 1981).
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 2-amino-4,6-dinitrophenol
- EC Number:
- 202-544-6
- EC Name:
- 2-amino-4,6-dinitrophenol
- Cas Number:
- 96-91-3
- Molecular formula:
- C6H5N3O5
- IUPAC Name:
- 2-amino-4,6-dinitrophenol
- Test material form:
- other: Dark red needles
- Details on test material:
- - Name of test material (as cited in study report): Picramic acid
- Molecular formula (if other than submission substance): C6H5N3O5
- Molecular weight (if other than submission substance): 199.125g\mol
- Smiles notation (if other than submission substance): c1(cc(cc(c1O)N)[N+](=O)[O-])[N+](=O)[O-]
- InChl (if other than submission substance): 1S/C6H5N3O5/c7-4-1-3(8(11)12)2-5(6(4)10)9(13)14/h1-2,10H,7H2
- Substance type: Organic
- Physical state: Dark red needles
- Analytical purity: No data available.
- Lot/batch No.:38C-0117
- Storage condition of test material: The stock solutions were stored in the dark at 5°C.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- yes
Test organisms
- Test organisms (species):
- other aquatic mollusc: Crassostrea virginica
- Details on test organisms:
- TEST ORGANISM
- Common name: American Or Virginia Oyster)
- Strain: virginica
- Source: Wilde Oyster Hatchery, Shady Side, Maryland
- Age at study initiation (mean and range, SD): Clutchless
- Weight at study initiation (mean and range, SD): No data available
- Length at study initiation (length definition, mean, range and SD): No data available
- Valve height at study initiation, for shell deposition study (mean and range, SD): 48.4 + 6.2 mm
- Peripheral shell growth removed prior to test initiation: No data available
- Method of breeding: No data available
- Feeding during test: Yes
- Food type: starch-yeast solution
- Amount: Fed 500 ml of 1.5 percent starch-yeast solution
- Frequency: Daily
ACCLIMATION
- Acclimation period: 2weeks
- Acclimation conditions (same as test or not): Yes
- Type and amount of food: Fed natural plankton in the estuarine water system supplemented with 1.5 percent corn starch-yeast solution when necessary.
- Feeding frequency: As per requirement.
- Health during acclimation (any mortality observed): Less than 3 percent mortality occurred in thelaboratory populations of oysters during acclimation.
Study design
- Test type:
- static
- Water media type:
- saltwater
- Total exposure duration:
- 144 h
Test conditions
- Test temperature:
- 10.5°C
- pH:
- 7.6
- Salinity:
- 7.0
- Nominal and measured concentrations:
- 0, 5, 15, 25, 35, and 75 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 38 l
- Aeration: Air-lift system was provided.
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): 24 hour
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates):6 concentration (one replicate)
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: No
- Intervals of water quality measurement:3 days
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 12 hlight:12 h dark
Results and discussion
Effect concentrations
- Duration:
- 144 h
- Dose descriptor:
- LC50
- Effect conc.:
- 69.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
Applicant's summary and conclusion
- Conclusions:
- LC50 was found to be 69.8 mg/l for Picramic acid in Crassostrea virginica for 144 hour
- Executive summary:
In Long-term toxicity to aquatic invertebrates for Picramic acid was determined in Crassostrea virginica for 144 hour. The LC50 was found to be 69.8 mg/l for Picramic acid.
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