Registration Dossier
Registration Dossier
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EC number: 260-480-4 | CAS number: 56966-52-0
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP-guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 5-chloro-2-(2,4-dichlorophenoxy)aniline
- EC Number:
- 260-480-4
- EC Name:
- 5-chloro-2-(2,4-dichlorophenoxy)aniline
- Cas Number:
- 56966-52-0
- Molecular formula:
- C12H8Cl3NO
- IUPAC Name:
- 5-chloro-2-(2,4-dichlorophenoxy)aniline
- Details on test material:
- - Name of test material (as cited in study report): FAT 80023/G
- Physical state: solid
- Analytical purity: 100%
- Purity test date: 100%
- Batch No.: ICMD CH 2.23
- Storage condition of test material: room temperature
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: mixture of polyvalent bacteria of the aeration tank
- Details on inoculum:
- - Source of inoculum/activated sludge: mixture of polyvalent bacteria in aaeration tank of a domestic sewage treatment plant
- Bacteria concentration: <= 30 mg/L suspended solids - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Test temperature: 22 ± 2 °C
- Lighting: none
- pH-value: 7.5 - 8.5
TEST SYSTEM
- Number of culture flasks/concentration: 2
- Measuring equipment: Voith Sapromat
SAMPLING
- Sampling frequency: At day 2, 4, 5, 6, 7, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxcicity control: yes
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- Biodegrdation:
85% after 28 days
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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