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EC number: 811-460-4 | CAS number: 53606-41-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-methoxy-5-nitrophenyl acetate
- Cas Number:
- 53606-41-0
- Molecular formula:
- C9H9NO5
- IUPAC Name:
- 2-methoxy-5-nitrophenyl acetate
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
Constituent 1
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- The water with conductivity smaller than 5 μS·cm-1 was used for the preparation of solutions. All chemicals used were of analytical-grade.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- The green freshwater algae Desmodesmus subspicatus Brinkmann 1953/SAG 86.81 obtained from the collection of autotrophic organisms of The Botanic Institute of the Czech Academy of Science, Třeboň on date 3.6.2014.
The sensitivity of test species is periodically monitored by testing with the reference substance, potassium dichromate. The strain culture was preinoculated from the stock solution and cultivated in flasks with the test medium on indirect daylight at laboratory temperature. Algae inoculum for the test was sampled from exponentially growing inoculum culture. The strain culture was always set to pre-culturing of cells for 3-4 days before the start of the test. Inoculum culture was kept 3-4 days under conditions at which the test was performed. After this period the culture was in the state of the exponential growth and had the cell density suitable for the performance of the test. The cell density of the pre-culture was measured just before the start of the test and the needed inoculum volume was calculated
Study design
- Test type:
- not specified
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22.5°C
- pH:
- 7.6
- Details on test conditions:
- Temperature: 21 - 24 °C controlled at ± 2 °C
Lighting: under continuous white light, 4 440 lux to 8 880 lux
Exposition time: 72 hours
Volume of tested mixture: 50 mL
Initial concentration of algae culture: 5 000 cells in a 1 mL
Without aeration.
Agitation of algae culture by shaking. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.53 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Basis for effect:
- growth rate
- Remarks on result:
- other: 1.04-2.38
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.27 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- The results of the verification test with K2Cr2O7, carried out in period from 30.3. to 2.4. 2015, are the following:
72 hour – ErC50 = 0.79 mg·L-1 (95% confidence limit: 0.70 – 0.89 mg·L-1)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
The test substance, 2-methoxy-5-nitrophenyl acetate, was tested for growth inhibition on algae Desmodesmus subspicatus. The test was performed according to method C.3. - Freshwater Algae and Cyanobacteria, Growth Inhibition Test, Commission Regulation (EC) No. 761/2009, Annex IV. The preliminary test was performed in range of test substance nominal concentrations 1 – 100 mg·L-1. The analytical results showed, that the test substance 2-methoxy-5-nitrophenyl acetate was not stable in the test medium at conditions of the test in concentration range 1 – 100 mg·L-1 . Based on the toxicity of the test substance found in preliminary test, the full test was performed in appropriate concentration range. The full test was performed in range of test substance nominal concentrations 5 – 62 mg·L-1. Samples for analytical determination were taken at the beginning, after 24 hours, 48 hours and at the end of the test. The analytical results showed that the test substance 2-methoxy-5-nitrophenyl acetate was not stable in test medium at conditions of the test. With respect to this observation the geometric mean concentrations were used for results presentation and evaluation. Test results: 72 hour – ErC50 = 1.53 mg·L-1 (geometric mean concentration) (95% confidence limit: 1.04 – 2.38 mg·L-1) 72 hour – EyC50 = 0.16 mg·L-1 (geometric mean concentration) (95% confidence limit: 0.02 – 0.38 mg·L-1) 72 hour – NOECr = 0.27 mg·L-1 (geometric mean concentration) 72 hour – NOECy < 0.18 mg.L-1
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