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EC number: 212-593-5 | CAS number: 830-03-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- yes
- Principles of method if other than guideline:
- There was a deviation from temperature in the preliminary test and in the definitive test. The temperature was in the prescribed range (20 ± 2°C) during test, but with a variation greater than 1 °C. The variance of temperatures was 2.2 °C (19.0 – 20.2 °C) during the preliminary test and the variance of temperatures was 2.1 °C (19.8 – 21.9 °C) during the definitive test. So the prescribed conditions of the test were not fulfilled in this point, but the variations were minimal and take only short period of time.
This deviation does not impact the integrity of study. - GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Acetic acid, 4-nitrophenyl ester
- IUPAC Name:
- Acetic acid, 4-nitrophenyl ester
- Reference substance name:
- 4-nitrophenyl acetate
- EC Number:
- 212-593-5
- EC Name:
- 4-nitrophenyl acetate
- Cas Number:
- 830-03-5
- Molecular formula:
- C8H7NO4
- IUPAC Name:
- 4-nitrophenyl acetate
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): 4-Nitrophenyl Acetate
- Physical state: solid
- Analytical purity: 95 % w/w
- Impurities (identity and concentrations):
Phenol <5.0 % (w/w)
4-nitrophenol <5.0 % (w/w)
Acetic acid <1.0 % (w/w)
Water <5.0 % (w/w)
- Lot/batch No.: 201409
- Expiration date of the lot/batch: unlisted
- Storage condition of test material: During the study the test substance was stored in closed
container in the dark at laboratory temperature.
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Details on test solutions:
- For holding and culturing of the daphnia the M4 medium was used (according to Annex 2 of the EU method C.2 and according to Annex 3 of the OECD TG 202).
For test was used dilution water (according to Annex 2 of the EU method C.2 and according to Annex 3 of the OECD TG 202). Dilution water was prepared just before the test by admixing 2.5 mL of stored solutions A, B, C and D into 1 000 mL of deionized water.
A. 117.6 g CaCl2·2H20 in 1 L of deionized water
B. 49.3 g MgSO4·7H2O in 1 L of deionized water
C. 25.9 g NaHCO3 in 1 L of deionized water
D. 2.3 g KCl in 1 L of deionized water
The dilution water is saturated by oxygen by the aeration at temperature of 18 – 22 °C.
It should be of pH 7.8 0.2, values measured in this study were 7.9 in preliminary test and 7.7 and 7.8 in the semistatic definitive test.
The content of sum Ca + Mg ions should have the value of 2.50 0.1 mmol·L-1, values measured in this study were 2.53 mmol·L-1 in the preliminary test and 2.54 mmol·L-1 and 2.52 mmol·L-1in the semistatic definitive test.
Measured values meet the requirements.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain: Straus
- Source: own breeding
- Method of breeding:
- Feeding during test
- Food type: mixture of algae
ACCLIMATION
- Acclimation period: 48 h
- Acclimation conditions (same as test or not): dilution water
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20 2°C, with a variation of 1°C at each individual test
- pH:
- 7.7 - 7.8
- Dissolved oxygen:
- higher than 3 mg·L-1
- Nominal and measured concentrations:
- The definitive semistatic test was performed with a range of nominal concentrations from 2.1 mg·L-1 to 10.0 mg·L-1.
Test solution 1 (prepared 01.7.2015)
Stock solution of test substance: 10.09 mg·L-1
Test concentration: 10.0, 7.7, 5.9, 4.5, 3.5, 2.7 and 2.1 mg·L-1
Conductivity of deionized water: 1.55 μS·cm-1
pH of dilution water: 7.7
Oxygen content of dilution water: 8.3 mg·L-1
Content of (Ca+Mg) in dilution water: 2.54 mmol·L-1
Test solution 2 (prepared 02.07.2015)
Stock solution of test substance: 10.09 mg·L-1
Test concentration: 10.0, 7.7, 5.9, 4.5, 3.5, 2.7 and 2.1 mg·L-1
Conductivity of deionized water: 1.55 μS·cm-1
pH of dilution water: 7.8
Oxygen content of dilution water: 8.3 mg·L-1
Content of (Ca+Mg) in dilution water: 2.52 mmol·L-1 - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.96 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- not specified
- Remarks on result:
- other: 3.04-4.88
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.78 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- not specified
- Remarks on result:
- other: 2.02-3.53
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 2.7 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- < 2.1 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 5.5 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 5.5 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The time weighted mean concentrations were used for all evaluations and results because was demonstrated that the concentrations of the test substance were not satisfactorily maintained within ± 20 percent of measured initial concentration throughout the test.
The EC0 and EC100 values are identified by direct observation. - Results with reference substance (positive control):
- The sensitivity of the test species and correctness of test performance is periodically verified in six-month period by testing with the reference substance, potassium dichromate.
The results of the verification test with K2Cr2O7, carried out in period from 15.04. to 17.04.2015 are the following:
24 hour – EC50 = 1.95 mg·L-1(95% confidence limit: 1.75 – 2.15 mg·L-1)
48 hour – EC50 = 0.86 mg·L-1(95% confidence limit: 0.70 – 1.02 mg·L-1)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance, 4-Nitrophenyl Acetate, was tested in acute immobilisation test on Daphnia magna.
- Executive summary:
The test substance, 4-Nitrophenyl Acetate, was tested in acute immobilisation test on Daphnia magna. The test was performed according to method C.2 – Daphnia sp. Acute Immobilisation Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008. The preliminary test was performed in range of the test substance nominal concentrations 1 – 100 mg·L-1. The analytical results showed, that the test substance 4-Nitrophenyl Acetate was not stable in dilution water in the conditions of the test. Based on toxicity and instability of the test substance found in preliminary test, the definitive test was performed in appropriate concentration range and as semistatic. The semistatic definitive test was performed in range of the test substance nominal concentrations 2.1 – 10.0 mg·L-1. The test solutions were renewed after 24 hours. The test substance concentrations was measured at the beginning, after 24 hours (before and after renewal) and at the end of the test. There is stated in the guideline that, if evidence is available to demonstrate that the concentration of the test substance has been satisfactorily maintained within ± 20 percent of the nominal or measured initial concentration throughout the test, the results can be based on nominal or measured initial values. Because concentrations of the test substance have not been satisfactorily maintained within ± 20 percent of the nominal or measured initial concentration throughout the test, time-weighted mean concentrations were used for all evaluations and results. Test results: 24 hour–EC50 = 3.96 mg·L-1 (time-weighted mean concentration) (95% confidence limit: 3.04 – 4.88 mg·L-1) 48 hour–EC50 = 2.78 mg·L-1 (time-weighted mean concentration) (95% confidence limit: 2.02 – 3.53 mg·L-1) 24 hour–EC0 = 2.7 mg·L-1 (time-weighted mean concentration) 48 hour–EC0
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