2-methylpropyl-(R)-2-hydroxypropanoate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-methylpropyl-(R)-2-hydroxypropanoate

EC Number:

407-770-0

EC Name:

2-methylpropyl-(R)-2-hydroxypropanoate

Cas Number:

61597-96-4

Molecular formula:

C7 H14 O3

IUPAC Name:

2-methylpropyl-(R)-2-hydroxypropanoate

Details on test material:

- Name of test material (as cited in study report): D-Milchsäureisobutylester
- Physical state: fluid
- Analytical purity: 99%
- Impurities: i-Butanol (< 1%)
- Batch No.: Cont. TTTU 119 618

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Stock solution: Tets substance was directly added. Nominal concentration of the stock solution = 100 mg/L

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: Originally from "Institut National de Recherche Chimique appliquee, France", and has been bred from 1978 in the Laboratories of BASF
- Age at study initiation: 2 - 24 h
- Feeding during test: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.44 mmol/L

Test temperature:

19.6 - 20.6 °C

pH:

7.7 - 8.0

Dissolved oxygen:

7.4 - 8.4 mg/L

Nominal and measured concentrations:

Nominal test concentrations: control, 6.25, 12.5, 25, 50, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, nominal volume, test volume: glass tubes with flat bottom, 20 ml, 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water
- Alkalinity (pH 4.3): 0.9 ± 0.5 mmol/L
- Ca/Mg ratio: ca. 4:1
- Conductivity: 600 - 700 µS/cm
- Intervals of water quality measurement: temperature after 0, 24, 48 h in seperate test vessels, pH and oxygen content in one replicate of each test concentration

OTHER TEST CONDITIONS
- Photoperiod: day : night interval 16 : 8 h
- Light intensity: ca. 5-6 µE/m²s, wavelength 400 - 700 nm

EFFECT PARAMETERS MEASURED: mobility after 0, 3, 6, 24, 48 hours

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility