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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Study period:
- 1982
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to valid methods and considered reliable, adequate and relevant. Limited details were available.
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenesis Testing of Acetyl-Tributylcitrate and Epoxidized Soybean Oil
- Author:
- Heath J.L. and Reilly M.
- Year:
- 1 982
- Bibliographic source:
- 1982 Poultry Science 61:2517-2519
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- without metabolic activation only
- Principles of method if other than guideline:
- In this case, enzyme activation was determined not to be necessary.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Soybean oil, epoxidized
- EC Number:
- 232-391-0
- EC Name:
- Soybean oil, epoxidized
- Cas Number:
- 8013-07-8
- IUPAC Name:
- 8013-07-8
- Reference substance name:
- Soybean oil,epoxidized
- IUPAC Name:
- Soybean oil,epoxidized
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report): Epoxidized soybean oil; ESO
- Molecular formula (if other than submission substance): C57H98O12 (typical structure example)
- Substance type: UVCB
Constituent 1
Constituent 2
Method
- Target gene:
- histidine
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium TA 98, TA 100, TA 1535, TA 1537 and TA 1538
- Metabolic activation:
- without
- Metabolic activation system:
- In these studies enzyme activation was determined not to be necessary. In the case of ESO, evidence indicates that compounds containing epoxides would be a possible carcinogen without metabolic activation (Miller and Miller, 1977; Sims, 1977)
- Test concentrations with justification for top dose:
- Epoxidized soybean oil (Dow Chemical Co.) (18.11 mg/2 mL dimethylsulfoxide) solutions containing 9, 18, 45, 90 and 453 µg were tested for all strains.
Controls
- Positive control substance:
- other: nitrofluorene solutions (1.46 mg/ 2mL dimethylsulfoxide)
- Remarks:
- 1.5, 2.9, 4.4, 11.0, and 54.8 µg were used with tester strain TA 98; 1.5 µg with TA 100; 1.5, 2.9, and 4.4 µg with TA 1535 and 11.0 and 54.8 µg with TA 1537 and 1538.
- Evaluation criteria:
- The total number of revertants to histidine protrophy per plate was recorded. Chemicals were considered to have a negative response if the number of induced revertants compared to the spontaneous reversion rate of the stain was less than twofold.
Results and discussion
Test results
- Species / strain:
- other: Salmonella typhimurium TA 98, TA 100, TA 1535, TA 1537 and TA 1538
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Mutagenesis can be grouped into two different classes, point mutation and frameshift mutations. The TA 100 and TA 1535 strains are used to detect base pair substitutions (point mutations) and strains TA 98, TA 1537 and TA 1538 to detect frameshift mutagens. The background number of revertants was low, > 25, for all strains except of TA 100. The background had none of the test compounds added. This number correlated with that reported by McCann and Ames (1976) who found that the spontaneous reversion rate was as high as 160 for control plates of TA 100 and a strain such as TA 1538 had < 30 revertants per plate. Nitrofluorene was used as a positive control and caused the TA 98 strain to have the highest number of revertants per plate. The number increased as the amount of test compound increased. The point mutation strain, TA 100 was at least as sensitive to nitrofluorene, 377 revertants, as TA 98. Tester strain TA 1537 was much less sensitive to nitrofluorene-induced mutagenesis than TA 98 and TA 100, whereas TA 1538 and TA 1535 were totally insensitive to nitrofluorene mutagenesis. Nitrofluorene thus served as a positive control in this system, because it elicited a response from both frameshift and point mutation strains.
ESO was not mutagenic in any of the tester strains used at any of the levels incorporated into the assay. The number of revertants produced after incorporation of these agentia into the system was not markedly different from control plates.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative without metabolic activation
ESBO was not mutagenic in any of the tester strains used at any of the levels incorporated into the assay. The number of revertants produced after incorporation of these agentia into the system was not markedly different from control plates. - Executive summary:
Bacterial mutagenicity testing was conducted on Epoxidized Soybean Oil (ESBO) in Salmonella typhimurium strains TA 98, TA 100, TA 1535, TA 1537 and TA 1538 without metabolic activation. In this case, enzyme activation was determined not to be necessary. ESBO was not mutagenic in any of the tester strains used at any of the levels incorporated into the assay. The number of revertants produced after incorporation of these agentia into the system was not markedly different from control plates.
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