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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From May 16, 2016 to November 19, 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- 1. Preparation of alga solution: According to OECD 201:2011, initial biomass of Pseudokirchneriella subcapitata was 1 x 10^4 cells/mL and must conduct acclimated culture to ensure it reached exponential growth in 2-4 days before the test start.
2. Preparation of test article: CJ303 was prepared and dissolved by medium. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- - Source (laboratory, culture collection): National Chiayi University Freshwater Bioresource Center
- Acclimation period: 2-4 days
Culture conditions:
1. Temperature: 21-24℃ and the temperature constant within ±2℃ between culture period.
2. Light intensity: Wavelength from the range of 400-700nm. The light intensity rom the range of 4440-8880 lux. The light intensity should be maintained within ±15%. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 21-24℃ and the temperature constant within ±2℃ between culture period.
- Details on test conditions:
- - Test vessel: 125 mL glass flask
- Test volume: 60 mL
- Type of cover: sealed with silicone plugs
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
- Light intensity and quality: Wavelength from the range of 400-700nm. The light intensity rom the range of 4440-8880 lux. The light intensity should be maintained within ±15%.
- Shaking rate: 100 ± 10 rpm. - Duration:
- 72 h
- Dose descriptor:
- EC50
- Remarks:
- ErC50
- Effect conc.:
- > 100 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Remarks:
- EyC50
- Effect conc.:
- 49.62 mg/L
- Validity criteria fulfilled:
- yes
- Conclusions:
- According to OECD 201 test method, the ErC50 and the EyC50 of CJ303 was >100 mg/L and 49.62 mg/L respectively.
- Executive summary:
An alga growth inhibition test was conducted according to OECD 201:2011 to evaluate growth inhibition caused by CJ303. The test system was Pseudokirchneriella subcapitata. According to OECD201:2011, the concentration series should cover the range causing 5-75% inhibition of alga growth rate. If there was no growth inhibition occurred at 100 mg/L, the highest concentration was 100 mg/L. In the range-finding test, the test article concentration of 100.00-0.16 mg/L was used which covered a range of -4.07~1.16% inhibition in average specific growth rate, so the limit test was conducted. In the limit test, the test article concentrations applied was 100.00 mg/L. The results showed that, in days 0-3, the percent inhibition in average specific growth rate was a range of 18.08~26.41%, and the percent inhibition of yield was a range o 53.66~67.67%. Therefore, under these test conditions, the ErC50 of CJ303 forPseudokirchneriella subcapitatawas greater than 100.00 mg/L and EyC50 was 49.62 mg/L.
Reference
Table 1. The algal biomass for the limit test
Group (mg/L) |
Mean of algal biomass (×104cells/mL) ± standard deviation |
||||
Day 0 |
Day 1 |
Day 2 |
Day 3 |
||
Control group |
0.00 |
1.00 ± 0.00 |
5.65 ± 0.47 |
23.22 ± 2.63 |
63.45 ± 2.97 |
Test group |
100.00 |
1.00 ± 0.00 |
4.53 ± 0.40 |
14.43 ± 2.41 |
26.43 ± 4.03 |
Table 2. The average specific growth rate for the limit test
Group (mg/L) |
Mean of average specific growth rate (d-1) ± standard deviation (Coefficient of variation [%]) |
||||
Day 0-1 |
Day 1-2 |
Day 2-3 |
Day 0-3 |
||
Control group |
0.00 |
1.73 ± 0.09 (4.99) |
1.471 ± 0.07 (5.27) |
1.01 ± 0.07 (7.09) |
1.38 ± 0.02 (1.16) |
Test group |
100.00 |
1.51 ± 0.09 (5.86) |
1.15 ± 0.13 (11.75) |
0.61 ± 0.12 (20.09) |
1.09 ± 0.05* (4.93) |
Table 3. The percent inhibition in average specific growth rate and percent inhibition of yield for the limit test
Group (mg/L) |
Logarithm of concentration (mg/L) |
Percent inhibition in average specific growth rate (%) |
Percent inhibition of yield (%) |
100.00 |
2.00 |
18.08 |
53.66 |
19.25 |
55.93 |
||
18.43 |
54.36 |
||
26.16 |
67.32 |
||
26.41 |
67.67 |
||
19.66 |
56.70 |
Table 4. ECXfor the limit test
Parameter |
ECX (mg/L) |
ErC10a |
46.88 |
ErC20 |
93.76 |
ErC50 |
>100.00 |
EyC10b |
16.87 |
EyC20 |
33.74 |
EyC50 |
84.36 |
a ErCxwas calculated by percent inhibition in average specific growth rate.
b EyCxwas calculated by percent inhibition of yield
Table 5. The pH value for the limit test
Group (mg/L) |
Mean of pH value ± standard deviation |
||
Day 0 |
Day 3 |
||
Control group |
0.00 |
7.99 ± 0.03 |
8.51 ± 0.11 |
Test group |
100.00 |
7.84 ± 0.10 |
7.99 ± 0.07 |
Description of key information
ErC50and the EyC50of CJ303 was >100 mg/L and 49.62 mg/L for Pseudokirchneriella subcapitata, respectively (OECD TG201).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
Additional information
An alga growth inhibition test was conducted according to OECD 201:2011 to evaluate growth inhibition caused by CJ303. The test system wasPseudokirchneriella subcapitata. According to OECD201:2011, the concentration series should cover the range causing 5-75% inhibition of alga growth rate. If there was no growth inhibition occurred at 100 mg/L, the highest concentration was 100 mg/L. In the range-finding test, the test article concentration of 100.00-0.16 mg/L was used which covered a range of -4.07~1.16% inhibition in average specific growth rate, so the limit test was conducted. In the limit test, the test article concentrations applied was 100.00 mg/L. The results showed that, in days 0-3, the percent inhibition in average specific growth rate was a range of 18.08~26.41%, and the percent inhibition of yield was a range o 53.66~67.67%. Therefore, under these test conditions, the ErC50of CJ303 forPseudokirchneriella subcapitatawas greater than 100.00 mg/L and EyC50was 49.62 mg/L.
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