Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-184-2 | CAS number: 104-20-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2011-02-25 to 2011-03-25
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal sewage treatment plant, D-31137 Hildesheim
- Pretreatment: the activated sludge was washed twice with autoclaved tap water. After the washing the settled sludge was filled up with mineral salts medium and maintained in an aerobic condition by aeration for 4 hours. Thereafter, the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition by aeration with CO2-free air for 6 days. 10mL/L were used to initiate inoculation.
- Colony forming units in the test vessels: 10E7 - 10E8 CFU/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 25 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral salts medium according to OECD 301F
- Test temperature: 21 - 22°C
- pH: 7.68
- Dispersion treatment: continuous stirring
- ThOD in test vessel: 60.5 mg O2/L
- Photoperiod: dark, in an incubator
- Other: application: once at the start, the test item (6.0µL/test vessel) was injected directly into the test solution.
TEST SYSTEM
- Test vessels: brown glass bottles (500 mL)
- Test volume: 250 mL
- Number of culture flasks/concentration: 2 for the test item (P1, P2); 1 for functional control (R1); 1 for toxicity control (T1); 2 for inoculum control (C1, C2)
- Test performed in closed vessels: bottles were closed with OxiTop measuring heads
- Details of trap for CO2 and volatile organics if used: a rubber sleeve with soda lime was hung into the opening of the bottles to absorb evolved CO2
- Test concentration: 25 mg/L test item
CONTROL AND BLANK SYSTEM
- Inoculum blank: test medium without test and/or reference item
- Toxicity control: test item (25 mg/L) and reference item (45 mg/L)
- Functional control: 45 mg/L reference item
STATISTICAL METHODS: Calculation of the ThOD. The oxygen depletion of the test and reference item were calculated. The biodegradation of the test and reference item was calculated out of the BOD in relation to the ThOD. - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- purity: 100 %
- Test performance:
- The pass level of biodegradation of the functional control was >60% after 3 days. The biodegradation rate came to a maximum of 96% on day 26. The validity criterion (> 60% after 14 days) was fulfilled.
The toxicity control achieved 87% biodegradation after 14 days. After 28 days the biodegradation came to 93%. The validation criteria that the test item should not inhibit the biodegradation of the reference item was fulfilled. - Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 100
- Sampling time:
- 28 d
- Details on results:
- The CFU of the inoculum was determined at the start by standard dilution plate count: 4.3 x 10 e9 CFU/L.
The biodegradation of the test item was 100% after 28 days. The replicates reached the 10% level (beginning of biodegradation) after 3 days, and the pass level of 60% was reached within the 10-d-window on day 5. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item is found to be readily biodegradable in the 10-d-window and after 28 days (100%).
- Executive summary:
The ready biodegradability of the test item was determined with a non adapted activated sludge in the Manometric Respirometry Test for a period of 28 days, according to the OECD test guideline 301F. The test item concentration was 25 mg/L, corresponding to a ThOD of 60.5 mg O2/ test vessel. The oxygen was depleted by the respiration of bacteria and the degradation was followed by measuring the oxygen concentration. The biodegradation rate is therefore expressed as the percentage BOD (biological oxygen demand) and was calculated for each study day.
The mean oxygen deletion in the inoculum was 7.8 mg O2/L on day 28.
In order to check the activity of the test system sodium benzoate was used as functional control. The pass level >60% was reached after 3 days. The biodegradation rate came to a maximum of 96% on day 26.
In the toxicity control containing both test and reference item 87% degradation occurred within 14 days. After 28 days the biodegradation came to 93%. The degradation of the reference item was not inhibited by the test item.
For the test item, the replicates reached the 10% level after 3 days, and the pass level of 60% was reached within the 10-d-window on day 5. After 28 days the mean biodegradation was 100%.
The test item is found to be readily biodegradable in the 10-d-window and after 28 days.
Reference
The biodegradation is described here:
Concentration | Replicate | Biodegradation (%) | ||||
day 7 | day 14 | day 21 | day 28 | |||
Test item | 25 mg/L | 1 | 81 | 91 | 97 | 100 |
2 | 88 | 98 | 100 | 100 | ||
Functional control | 45 mg/L | 1 | 82 | 90 | 98 | 95 |
Toxicity control | 25 mg/L test item + 45 mg/L reference item | 1 | 74 | 87 | 91 | 93 |
Description of key information
The ready biodegradability of the test item was determined for a period of 28 days, according to the OECD test guideline 301F. The test item is found to be readily biodegradable in the 10-d-window and after 28 days.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
The ready biodegradability of the test item was determined with a non adapted activated sludge in the Manometric Respirometry Test for a period of 28 days, according to the OECD test guideline 301F. The 10% level was reached after 3 days and the pass level of 60% was reached within the 10-d window, i.e. on day 5. After 28 days the mean biodegradation was 100%. The test item is classified as readily biodegradable fulfilling the 10-d window. Moreover, in the toxicity control, the degradation of the reference item was not inhibited by the test item.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.