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EC number: 285-203-4 | CAS number: 85049-34-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 Jan - 12 Apr 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- GLP-Guideline study with acceptable restrictions (test substance was slightly degraded in the highest test concentration)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: stock solution - control - 0.59 - 0.99 - 1.8 - 2.9 - 5.3 mg/L
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 1000 mg of the test substance was dissolved in 1 L deionised water and stirred for approx. 18 h. The solution was filtered and nominal test substance concentrations were calculated based on the DOC content of stock solution.
- Eluate: No
- Differential loading: No
- Controls: yes, algae growth medium control - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: CHODAT (86.81 SAG)
- Source (laboratory, culture collection): Initially obtained from "Institut für Wasser-, Boden- und Lufthygiene Berlin, Germany.
- Method of cultivation: 3 days before the start of the study a pre-culture was prepared by inoculating from a stock culture (cell density: approx. 20000 cells/mL). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 22.5 - 22.8 °C
- pH:
- 7.9 - 8.3 (at the beginning)
8.7 - 9.2 (at test termination) - Nominal and measured concentrations:
- nominal: control - 0.59 - 0.99 - 1.8 - 2.9 - 5.3 mg/L
measured:
control: 0 mg/L (0 h)
0.59 mg/L test concentration: 0.085 mg/L (0 h), 1.01 mg/L (72 h)
0.99 mg/L test concentration: 0.82 mg/L (0 h), 0.97 mg/L (72 h)
1.8 mg/L test concentration: 1.60 mg/L (0 h), 1.52 mg/L (72 h)
2.9 mg/L test concentration: 3.05 mg/L (0 h), 2.22 mg/L (72 h)
5.3 mg/L test concentration: 5.77 mg/L (0 h), 2.81 mg/L (72 h) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Initial cells density: 2E+04 cells/mL
- Control end cells density: 65^10-4 cells/mL
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 8
GROWTH MEDIUM
- Standard medium used: yes, according to guideline
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Light intensity and quality: approx. 8000 lux white light
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: Photometric determination of the absorbance at 685 nm to determine cell concentrations after 0, 24, 48 and 72 h; the values were expressed in cells/mL with a calibration curve. - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 5.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (WAF)
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 5.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (WAF)
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Validity criteria fulfilled:
- yes
- Conclusions:
- After 72 hours the EL50 was determined to be greater than 5.3 mg/L and the NOELR was determined to be 5.3 mg/L.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22 Jul - 28 Aug 1997
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- GLP Guideline study with acceptable restrictions (Calculation of test concentration, No information on filter).
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Stock solution was sampled/measured once prior testing.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution of 1 g/L was prepared, stirred for 18 h and was subsequently filtered.
- Eluate: no
- Differential loading: no
- Controls: yes, water control - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: CHODAT 86.81 SAG
- Source: Own breeding. Originally obtained from the Institut für Wasser-, Boden-, und Lufthygiene, Berlin, Germany.
ACCLIMATION
- Acclimation period: 3 d
- Culturing media and conditions: sames as test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 23.5 - 23.8 °C
- pH:
- test start: 7.7 - 7.8
test end: 9.3 - Nominal and measured concentrations:
- nominal: 0, 1000 mg/L
measured: 3 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: sterile conical flasks
- Initial cells density: 2x10^4 cells/mL
- Control end cells density: 93x10^4
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 8
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline 92/69/EWG C.3
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: continuous light
- Light intensity and quality: approx. 8000 lux, white
EFFECT PARAMETERS MEASURED: Inhibition of growth based on the cell growth and growth rate after 24, 48 and 72 h.
- Determination of cell concentrations: The cell count was determined photometrically at a wavelength of 685 nm. - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- > 3 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Remarks:
- (WAF)
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 3 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Remarks:
- (WAF)
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- > 3 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Remarks:
- (WAF)
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 3 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Remarks:
- (WAF)
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control: yes
- Any stimulation of growth found in any treatment: yes, in the test concentration of 3.0 mg/L. - Validity criteria fulfilled:
- yes
- Conclusions:
- After 72 hours the EL50 was determined to be > 3 mg/L.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 22 Jul - 28 Augl 1997
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- GLP Guideline study with acceptable restrictions (Calculation of test concentration, No information on filter).
- Justification for type of information:
- Please refer section 13 for read across justification.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Stock solution was sampled/measured once prior testing.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution of 1 g/L was prepared, stirred for 18 h and was subsequently filtered.
- Eluate: no
- Differential loading: no
- Controls: yes, water control - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: CHODAT 86.81 SAG
- Source: Own breeding. Originally obtained from the Institut für Wasser-, Boden-, und Lufthygiene, Berlin, Germany.
ACCLIMATION
- Acclimation period: 3 d
- Culturing media and conditions: sames as test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 23.5 - 23.8 °C
- pH:
- test start: 7.7 - 7.8
test end: 9.3 - Nominal and measured concentrations:
- nominal: 0, 1000 mg/L
measured: 3 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: sterile conical flasks
- Initial cells density: 2x10^4 cells/mL
- Control end cells density: 93x10^4
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 8
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline 92/69/EWG C.3
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: continuous light
- Light intensity and quality: approx. 8000 lux, white
EFFECT PARAMETERS MEASURED: Inhibition of growth based on the cell growth and growth rate after 24, 48 and 72 h.
- Determination of cell concentrations: The cell count was determined photometrically at a wavelength of 685 nm. - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- > 3 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Remarks:
- (WAF)
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 3 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Remarks:
- (WAF)
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- > 3 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Remarks:
- (WAF)
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 3 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Remarks:
- (WAF)
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control: yes
- Any stimulation of growth found in any treatment: yes, in the test concentration of 3.0 mg/L. - Validity criteria fulfilled:
- yes
- Conclusions:
- After 72 hours the EL50 was determined to be > 3 mg/L.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 10 Jan - 12 Apr 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- GLP-Guideline study with acceptable restrictions (test substance was slightly degraded in the highest test concentration)
- Justification for type of information:
- Please refer section 13 for read across justification.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: stock solution - control - 0.59 - 0.99 - 1.8 - 2.9 - 5.3 mg/L
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 1000 mg of the test substance was dissolved in 1 L deionised water and stirred for approx. 18 h. The solution was filtered and nominal test substance concentrations were calculated based on the DOC content of stock solution.
- Eluate: No
- Differential loading: No
- Controls: yes, algae growth medium control - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: CHODAT (86.81 SAG)
- Source (laboratory, culture collection): Initially obtained from "Institut für Wasser-, Boden- und Lufthygiene Berlin, Germany.
- Method of cultivation: 3 days before the start of the study a pre-culture was prepared by inoculating from a stock culture (cell density: approx. 20000 cells/mL). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 22.5 - 22.8 °C
- pH:
- 7.9 - 8.3 (at the beginning)
8.7 - 9.2 (at test termination) - Nominal and measured concentrations:
- nominal: control - 0.59 - 0.99 - 1.8 - 2.9 - 5.3 mg/L
measured:
control: 0 mg/L (0 h)
0.59 mg/L test concentration: 0.085 mg/L (0 h), 1.01 mg/L (72 h)
0.99 mg/L test concentration: 0.82 mg/L (0 h), 0.97 mg/L (72 h)
1.8 mg/L test concentration: 1.60 mg/L (0 h), 1.52 mg/L (72 h)
2.9 mg/L test concentration: 3.05 mg/L (0 h), 2.22 mg/L (72 h)
5.3 mg/L test concentration: 5.77 mg/L (0 h), 2.81 mg/L (72 h) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Initial cells density: 2E+04 cells/mL
- Control end cells density: 65^10-4 cells/mL
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 8
GROWTH MEDIUM
- Standard medium used: yes, according to guideline
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Light intensity and quality: approx. 8000 lux white light
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: Photometric determination of the absorbance at 685 nm to determine cell concentrations after 0, 24, 48 and 72 h; the values were expressed in cells/mL with a calibration curve. - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 5.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (WAF)
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 5.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- (WAF)
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Validity criteria fulfilled:
- yes
- Conclusions:
- After 72 hours the EL50 was determined to be greater than 5.3 mg/L and the NOELR was determined to be 5.3 mg/L.
Referenceopen allclose all
No growth inhibitory effects were observed even at the highest test concentration. Therefore, the NOEC based on nominal concentrations is >= 5.3 mg/L. But the measured concentrations did not remain within 80 -120% of nominal. Therefore, the effect concentration should be based on the geometric mean of the measured concentrations at the start and at the end of the test, resulting in a NOEC >= 4.02 mg/L and an EC50 of > 4.02 mg/L.
Table 1: Nominal and measured concentrations
nominal concentrations |
measured concentrations after 0 h [mg/L] |
measured concentrations after 72 h [mg/L] |
filtered stock solution |
5.85 |
-- |
control |
0 |
-- |
0.59 |
0.085 |
1.01 |
0.99 |
0.82 |
0.97 |
1.9 |
1.6 |
1.52 |
2.9 |
3.05 |
2.22 |
5.3 |
5.77 |
2.81 |
No growth inhibitory effects were observed even at the highest test concentration. Therefore, the NOEC based on nominal concentrations is >= 5.3 mg/L. But the measured concentrations did not remain within 80 -120% of nominal. Therefore, the effect concentration should be based on the geometric mean of the measured concentrations at the start and at the end of the test, resulting in a NOEC >= 4.02 mg/L and an EC50 of > 4.02 mg/L.
Table 1: Nominal and measured concentrations
nominal concentrations |
measured concentrations after 0 h [mg/L] |
measured concentrations after 72 h [mg/L] |
filtered stock solution |
5.85 |
-- |
control |
0 |
-- |
0.59 |
0.085 |
1.01 |
0.99 |
0.82 |
0.97 |
1.9 |
1.6 |
1.52 |
2.9 |
3.05 |
2.22 |
5.3 |
5.77 |
2.81 |
Description of key information
No toxic effects to aquatic algae up to the limit of water solubility.
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 3 mg/L
Additional information
Since no studies investigating the toxicity to aquatic algae and cyanobacteria of the test substance are available, in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5 a read across conducted to the two structurally related substances butylene glycol dicaprylate / dicaprate (CAS 853947-59-8) and decanoic acid, mixed diesters with octanoic acid and propylene glycol (CAS 68583-51-7). This read-across is justified in detail in the overall summary (IUCLID chapter 6.1) and within the analogue justification in IUCLID Section 13.
The study with butylene glycol dicaprylate / dicaprate was performed as a limit test according to EU Method C.3 using the freshwater algae Scenedesmus subspicatus (Scholz, 1997). A test substance concentration of 1000 mg/L (nominal) and 3 mg/L (measured, based on TOC content) respectively, did not result in any effects on the growth rate and biomass after 72 h resulting in an EL10/50 > 3 mg/L (measured, initial). The recovery rate in the stability test was > 80% and the concentration after 48 and 72 h did not differ more than 20%. Therefore, the test substance was bioavailable throughout the test.
The second study with the read-across substance decanoic acid, mixed diesters with octanoic acid and propylene was performed according to the EU Method C.3 under GLP conditions (Scholz, 1995). The green algae Scenedesmus subspicatus was exposed to a Water Accommodated Fraction (WAF) of nominal 5.3 mg/L and subsequent dilutions for 72 h. No effects on the growth rate of S. subspicatus up to the limit of water solubility were observed resulting in an ELr50 > 5.3 mg/L and a NOELR of ≥ 5.3 mg/L.
Based on the results from structurally related read-across substances (in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5) which are characterized by a similar ecotoxicological profile, it can be concluded that the test substance will not exhibit toxic effects to aquatic algae up to the limit of water solubility.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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