Hydrogen [29H,31H-phthalocyaninesulphonato(3-)-N29,N30,N31,N32]cuprate(1-)

Endpoint summary

Description of key information

Stability:

According to annex VIII column 2 the study does not need to be conducted because the substance Hydrogen [29H,31H-phthalocyanine sulphonato(3-)-N29,N30,N31,N32]cuprate(1-) (CAS no. 28901 -96 -4) is highly insoluble in water.

Biodegradation:

Biodegradation in water:

Estimation Programs Interface Suite (EPI suite, 2018) was run to predict the biodegradation potential of the test compound Hydrogen [29H,31H-phthalocyanine sulphonato(3-)-N29,N30,N31,N32]cuprate(1-) (CAS no.  28901 -96 -4 ) in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical  Hydrogen [29H,31H-phthalocyanine sulphonato(3-)-N29,N30,N31,N32]cuprate(1-) is expected to be not readily biodegradable.

Bioaccumulation:

Bioaccumulation: aquatic/sediments:

Using BCFBAF Program (v3.00) model of EPI suite (2018) the estimated bio concentration factor (BCF) for Hydrogen [29H,31H-phthalocyanine sulphonato(3-)-N29,N30,N31,N32]cuprate(1-)(CAS No.28901-96-4) is 3.162  L/kg wet-wt at 25 deg. c which does not exceed the bioconcentration threshold of 2000. Therefore it is concluded that test chemical Hydrogen [29H,31H-phthalocyanine sulphonato(3-)-N29,N30,N31,N32]cuprate(1-) is non bioaccumulative in food chain.

Transport and distribution:

Bioaccumulation: aquatic/sediments:

Using BCFBAF Program (v3.00) model of EPI suite (2018) the estimated bio concentration factor (BCF) for Hydrogen [29H,31H-phthalocyanine sulphonato(3-)-N29,N30,N31,N32]cuprate(1-)(CAS No.28901-96-4) is 3.162  L/kg wet-wt at 25 deg. c which does not exceed the bioconcentration threshold of 2000. Therefore it is concluded that test chemical Hydrogen [29H,31H-phthalocyanine sulphonato(3-)-N29,N30,N31,N32]cuprate(1-) is non bioaccumulative in food chain.

Additional information

Stability:

According to annex VIII column 2 the study does not need to be conducted because the substance Hydrogen [29H,31H-phthalocyanine sulphonato(3-)-N29,N30,N31,N32]cuprate(1-) (CAS no. 28901 -96 -4) is highly insoluble in water.

Biodegradation:

Biodegradation in water:

Predicted data study for target chemical Hydrogen [29H,31H-phthalocyanine sulphonato(3-)-N29,N30,N31,N32]cuprate(1-) (CAS no.  28901 -96 -4 ) and experimental studies for its read across chemicals have been reviewed for biodegradation endpoint and their results are summarized below.

 

The first study was predicted data study in this the Estimation Programs Interface Suite (EPI suite, 2018) was run to predict the biodegradation potential of the test compound Hydrogen [29H,31H-phthalocyanine sulphonato(3-)-N29,N30,N31,N32]cuprate(1-) (CAS no.  28901 -96 -4 ) in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that chemical  Hydrogen [29H,31H-phthalocyanine sulphonato(3-)-N29,N30,N31,N32]cuprate(1-) is expected to be not readily biodegradable.

 

Next study was experimental study reviewed from study report in this the 42-days Closed Bottle test following the OECD guideline 301 D was performed to determine the ready biodegradability of the test chemical. The study was performed at a temperature of 20°C. The test system included control, test item and reference item. Polyseed were used for this study. 1 polyseed capsule were added in 500 ml D.I water and then stirred for 1 hour for proper mixing and functioning of inoculum. This gave the bacterial count as 10E7 to 10E8 CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l. OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 79.51%. Degradation of Sodium Benzoate exceeds 34.33% on 7 days & 61.44% on 14th day. The activity of the inoculum was thus verified and the test can be considered as valid. The BOD42 value of test chemical was observed to be 0.35 mgO2/mg. ThOD was calculated as 1.68 mgO2/mg. Accordingly, the % degradation of the test item after 42 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 20.83%. Based on the results, the test chemical, under the test conditions,was considered to benot readily biodegradable in nature.

 

Next experimental study was reviewed from authoritative database (J check, 2018) in this study the Biodegradation experiment was conducted for 14 days for evaluating the percentage biodegradability of test substance. The Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was determined to be 0% by BOD and 0.7% by UV-Vis in 14 days. Thus, the substance is not expected to be readily biodegradable in water.

 

By considering results of all the studies mentioned above it can be concluded that test chemical is not readily biodegradable in nature.

Bioaccumulation:

Bioaccumulation: aquatic/sediments:

Using BCFBAF Program (v3.00) model of EPI suite (2018) the estimated bio concentration factor (BCF) for Hydrogen [29H,31H-phthalocyanine sulphonato(3-)-N29,N30,N31,N32]cuprate(1-)(CAS No.28901-96-4) is 3.162  L/kg wet-wt at 25 deg. c which does not exceed the bioconcentration threshold of 2000. Therefore it is concluded that test chemical Hydrogen [29H,31H-phthalocyanine sulphonato(3-)-N29,N30,N31,N32]cuprate(1-) is non bioaccumulative in food chain.

Transport and distribution:

Adsorption/desorption:

Predicted data study for target chemical Hydrogen [29H,31H-phthalocyanine sulphonato(3-)-N29,N30,N31,N32]cuprate(1-) (CAS no.  28901 -96 -4 ) and experimental studies for its read across chemicals have been reviewed for Adsorption endpoint and their results are summarized below.

 

In the first study the Soil Adsorption Coefficient i.e. Koc value of test chemical Hydrogen [29H,31H-phthalocyanine sulphonato(3-)-N29,N30,N31,N32]cuprate(1-) (CAS No 28901-96-1 ) was estimated using EPI suite KOCWIN Program (v2.00) as 9850000000 L/kg (log Koc= 9.9934 ) by means of MCI method at 25 deg. C.  This log Koc value indicates that test chemical Hydrogen [29H,31H-phthalocyanine sulphonato(3-)-N29,N30,N31,N32]cuprate(1-) has very strong sorption to soil and therefore test chemical has negligible migration potential to ground water.

 

Next study was experimental study reviewed from authoritative database (HSBD, 2018) in this the adsorption coefficient Koc values of test chemical was determined to be in range 15,600 to 136,000 (Log Koc = 4.1931- 5.1335) in four types of soils (clay, sand, sandy clay loam and loam). According to a classification scheme, this Koc range suggests that test chemical is expected to be immobile in soil.

 

Next study was also experimental study reviewed from authoritative database (HSBD, 2018) in this adsorption experiment was conducted for estimating the adsorption coefficient (Koc) value of test chemical. The adsorption coefficient (Koc) value was calculated using measured log Kow of 5.5and a recommended regression-derived equation. The adsorption coefficient (Koc) value of test substance was estimated to be 43200 (Log Koc = 4.3692). This Koc value indicates that test chemical is expected to have no mobility in soil.

 

 By considering results of all the studies mentioned above it can be concluded that test chemical Hydrogen [29H,31H-phthalocyanine sulphonato(3-)-N29,N30,N31,N32]cuprate(1-) (CAS No 28901-96-1 ) has very strong sorption to soil and therefore test chemical has negligible migration potential to ground water.