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EC number: 203-294-0 | CAS number: 105-39-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 11.01.1983 to 24-02-1983
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 472 (Genetic Toxicology: Escherichia coli, Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Ethyl chloroacetate
- EC Number:
- 203-294-0
- EC Name:
- Ethyl chloroacetate
- Cas Number:
- 105-39-5
- Molecular formula:
- C4H7ClO2
- IUPAC Name:
- ethyl 2-chloroacetate
- Details on test material:
- - Name of test material (as cited in study report): Monochloressigsäureethylester
- Physical state: colourless, clear
- Analytical purity: 99.5%
- Impurities (identity and concentrations): ca. 0.1 % Etnanol; ca. 0.1 % Water; ca. 0.3 % Dichloressigsäuremethylester (Methyl-2,2-dichloroacetate)
- Lot/batch No.: LKW Reitter August 2, 1982
- Storage condition of test material: dark at 2 ºC
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 mix
- Test concentrations with justification for top dose:
- 4, 20, 100, 500, 2500 and 5000 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: At the day of the experiment the test substance was dissolved in DMSO at appropriate concentrations.
Controlsopen allclose all
- Negative solvent / vehicle controls:
- yes
- Remarks:
- (DMSO (100 µL))
- Remarks:
- with and without metabolic activation
- Positive controls:
- yes
- Remarks:
- (without metabolic activation)
- Positive control substance:
- sodium azide
- Remarks:
- TA 100 and TA 1535; Sodium azide: 1 µg/plate
- Positive controls:
- yes
- Remarks:
- ( without metabolic activation)
- Positive control substance:
- 9-aminoacridine
- Remarks:
- TA 1537; 9-Aminiacridine: 50 µg/plate
- Positive controls:
- yes
- Remarks:
- (without metabolic activation)
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- TA 1538 and TA 98; 2-nitrofluorene: 5 µg/plate
- Positive controls:
- yes
- Remarks:
- (without metabolic activation)
- Positive control substance:
- other: N-methyl-N'-nitro-N-nitrosoguanidine
- Remarks:
- WP2uvrA; N-methyl-N'-nitro-N-nitrosoguanidine: 5 µg/plate
- Positive controls:
- yes
- Remarks:
- (with metabolic activation)
- Positive control substance:
- benzo(a)pyrene
- Remarks:
- 10 µg/plate
- Positive controls:
- yes
- Remarks:
- (with metabolic activation)
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- TA 100, TA 1538 and TA 98: 0.5 µg/plate; TA 1535 and TA 1537: 1 µg/plate; WP2uvrA: 10 µg/plate
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation).
Top agar is prepared for the Salmonella strains by mixing 100 mL agar (0,6 % agar, 0,5 % NaCl) with 10 ml of a 0,5 mM histidine-biotin solution. With
E. coli histidine is replaced by tryptophan (2,5 mL, 0,5 mM). The following ingredients are added (in order) to 2 mL of molten top agar at 45°C:
0,1 mL test compound solution
0,1 mL of an overnight nutrient broth culture of the bacterial tester strain
0,5 mL S-9 Mix (if required) or buffer
After mixing, the liquid is poured into a petridish with minimal agar (1,5 % agar, Vogel-Bonner E medium with 2 % glucose).
DURATION
- Exposure duration: 48-72 h
NUMBER OF REPLICATIONS: Triplicates - Evaluation criteria:
- The test item is considered positive if there is a dose related increase in the number of revertants or a biologically relevant increase for at least one test concentration.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- (dose: 10000 µg/plate)
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- (dose: 10000 µg/plate)
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- (dose: 10000 µg/plate)
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES:
Preliminary toxicity tests were performed with all tester strains using a small number of plates to calculate an appropriate dose range. A reduced rate of spontaneously occuring colonies as well as visible thinning of the bacterial lawn were used as indicator for toxicity. Thinning of the bacterial lawn was controlled microscopically. The test compound was tested at doses of 4 to 10000 µg/plate and proved to be toxic to all bacterial strains at 10000 µg/plate. Thinning of the bacterial lawn and a reduction in the number of colonies has been observed at
10000 µg/plate.
COMPARISON WITH HISTORICAL CONTROL DATA: Control plates without mutagen showed that the number of spontaneous revertant colonies was similar to that described in the literature. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
In the absence of the metabolic activation system the test compound did not show a dose dependent influence in the number of revertants in any of the bacterial strains. Also in the presence of metabolic activation system, treatment of the cells with Monochloressigsäureethylester did not result in relevant increases in the number of revertant colonies. So it is considered that the test substance is not mutagenic in these bacterial test systems neither with nor without exogenous metabolic activation at the dose levels investigated.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Mutagenicity: In the absence of the metabolic activation system the test compound did not show a dose dependent influence in the number of revertants in any of the bacterial strains. Also in the presence of metabolic activation system, treatment of the cells with Monochloressigsäureethylester did not result in relevant increases in the number of revertant colonies. So it is considered that the test substance is not mutagenic in these bacterial test systems neither with nor without exogenous metabolic activation at the dose levels investigated. - Executive summary:
Monochloressigsäureethylester was tested for mutagenicity with the strains TA 100, TA 1535, TA 1537, TA 1538, TA 98 of Salmonella typhimurium and Escherichia coli WP2uvrA. The mutagenicity studies were conducted in the absence and in the presence of a metabolizing system derived from rat liver homogenate. A dose range of 6 different doses from 4 µg/plate to 5000 µg/plate was used.
Control plates without mutagen showed that the number of spontaneous revertant colonies was similar to that described in the literature. All the positive control compounds gave the expected increase in the number of revertant colonies.
Toxicity: The test compound proved to be toxic to the bacteria at 5000 and 10000 µg/plate. 5000 µg/plate was chosen as top dose level for the mutagenicity study.
Mutagenicity: In the absence of the metabolic activation system the test compound did not show a dose dependent influence in the number of revertants in any of the bacterial strains. Also in the presence of metabolic activation system, treatment of the cells with Monochloressigsäureethylester did not result in relevant increases in the number of revertant colonies.
Summarizing, it can be stated that Monochloressigsäureethylester is not mutagenic in these bacterial test systems neither with nor without exogenous metabolic activation at the dose levels investigated.
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