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EC number: 203-624-3 | CAS number: 108-87-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Repeated dose toxicity: dermal
Administrative data
- Endpoint:
- short-term repeated dose toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Only limited information given.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- publication
- Title:
- The physiological response of rabbits to cyclohexane, methylcyclohexane, and certain derivatives of these compounds. I Oral Administration and Cutaneous Administration
- Author:
- Treon, J. et al.
- Year:
- 1 943
- Bibliographic source:
- J. Indust. Hygiene Toxicol. 25(6), 199-214
Materials and methods
- Principles of method if other than guideline:
- Subacute (6-day) dermal toxicity study in one rabbit
- GLP compliance:
- no
Test material
- Reference substance name:
- Methylcyclohexane
- EC Number:
- 203-624-3
- EC Name:
- Methylcyclohexane
- Cas Number:
- 108-87-2
- Molecular formula:
- C7H14
- IUPAC Name:
- methylcyclohexane
- Details on test material:
- - Name of test material (as cited in study report): methylcyclohexane
- Substance type: pure substance
- Physical state: colourless liquid
- Analytical purity: 97%
- Impurities (identity and concentrations): toluene, 3%
- Specific gravity: 0.767
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- other: White
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: ca. 3.185 kg (calculated from the reported results)
Administration / exposure
- Type of coverage:
- open
- Vehicle:
- unchanged (no vehicle)
- Details on exposure:
- TEST SITE
- Area of exposure: approx. 24 square inches (corresponding to ca. 155 cm²) of the anterior abdominal wall
- Time intervals for shavings or clipplings: the hair of the animal was closely clipped prior to the first application.
REMOVAL OF TEST SUBSTANCE
- Washing (if done): the test material was removed by washing with soap and water.
- Time after start of exposure: 1 h
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 60 mL (twelve 5 mL portions at 5 min intervals)
- Constant volume or concentration used: yes
USE OF RESTRAINERS FOR PREVENTING INHALATION: yes - Analytical verification of doses or concentrations:
- no
- Duration of treatment / exposure:
- 6 days
- Frequency of treatment:
- daily, 12 applications within 1 h
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
60 mL per 24 square inches per day (specific gravity 0.767)
Basis:
other: reported data
- Remarks:
- Doses / Concentrations:
ca. 300 mg/cm²/day
Basis:
nominal per unit area
- Remarks:
- Doses / Concentrations:
ca. 14450 mg/kg bw/day
Basis:
nominal per unit body weight
- No. of animals per sex per dose:
- 1 animal
- Control animals:
- no
Examinations
- Observations and examinations performed and frequency:
- CAGE SIDE OBSERVATIONS: Yes
- Time schedule: daily
DERMAL IRRITATION: Yes
- Time schedule for examinations: daily
BODY WEIGHT: Yes
- Time schedule for examinations: presumably daily (not specified)
RECTAL TEMPERATURE: Yes
- Time schedule for examinations: presumably daily (not specified)
Results and discussion
Results of examinations
- Clinical signs:
- effects observed, treatment-related
- Description (incidence and severity):
- slight hypothermia, no mortality
- Dermal irritation:
- effects observed, treatment-related
- Description (incidence and severity):
- local irritation, skin hardening, thickening and ulceration
- Mortality:
- mortality observed, treatment-related
- Description (incidence):
- slight hypothermia, no mortality
- Body weight and weight changes:
- effects observed, treatment-related
- Description (incidence and severity):
- slight loss in weight
- Food consumption and compound intake (if feeding study):
- not examined
- Food efficiency:
- not examined
- Water consumption and compound intake (if drinking water study):
- not examined
- Ophthalmological findings:
- not examined
- Haematological findings:
- not examined
- Clinical biochemistry findings:
- not examined
- Urinalysis findings:
- not examined
- Behaviour (functional findings):
- not examined
- Organ weight findings including organ / body weight ratios:
- not examined
- Gross pathological findings:
- not examined
- Histopathological findings: non-neoplastic:
- not examined
- Histopathological findings: neoplastic:
- not examined
- Details on results:
- CLINICAL SIGNS AND MORTALITY
The treated animal did not die and slight hypothermia (not further specified) was observed during the 6-day study period.
DERMAL IRRITATION
Repeated application of methylcyclohexane to the skin of a rabbit induced local irritation and thickening. The irritation appeared on the second day and increased with succesive treatments. Hardening of the skin, thickening and ulceration appeared later and the experiment was terminated after the 6th day.
BODY WEIGHT AND WEIGHT GAIN
The treated animal showed a slight loss in weight (not further specified) during the 6 day-study period. The loss in body weight was regained within 2 days.
Effect levels
open allclose all
- Dose descriptor:
- conc. level:
- Effect level:
- 300 mg/cm² per day (nominal)
- Based on:
- test mat.
- Sex:
- not specified
- Dose descriptor:
- dose level:
- Effect level:
- 14 450 mg/kg bw/day (nominal)
- Based on:
- test mat.
- Sex:
- not specified
- Basis for effect level:
- other: succesively increasing local irritation; hardening of the skin, thickening and ulceration appearing within 6 days; slight hypothermia and loss in weight
Target system / organ toxicity
- Critical effects observed:
- not specified
Any other information on results incl. tables
The authors reported the "range of lethal dosage" to be > 86.7 g/kg bw.
This is evidently a cumulated dose calculated over the 6 -day study period:
60 mL per day = 46.02 g per day (based on a specific gravity of 0.767)
46.02 g x 6 days = 276.12 g
A cumulated dose of 86.7 g/kg bw is thus calculated assuming a body weight of ca. 3.185 kg.
Applicant's summary and conclusion
- Conclusions:
- Methylcyclohexane was applied to the clipped skin of one rabbit in twelve 5 ml portions at 5 min intervals within 1 h, after which the material was removed by washing with soap and water. This uncovered skin procedure was done on 6 consecutive days. Based on the reported total amount of test substance applied daily (60 mL), the specific gravity (0.767) and the animal body weight (3.185 kg), the applied dose was roughly equivalent to 14450 mg/kg bw/day. The test substance was applied on a surface of approximately 24 square inches (corresponding to ca. 155 cm²). Thus, the local dose was about 300 mg/cm²/day.
Slight hypothermia and a slight loss in body weight (no further details given) were observed in the treated animal during the 6-day study period. The loss in body weight was regained within 2 days. Repeated application of methylcyclohexane to the skin of a rabbit induced local irritation and thickening. The irritation appeared on the second day and increased with successive treatments. Hardening of the skin, thickening and ulceration appeared later and the experiment was terminated after the 6th day.
Due to deficiencies in study design (one animal, one dose level, 6-day exposure) and limited documentation, the results of this study are not fully sufficient for assessment of toxicity after repeated dermal exposure, not adequate for the purpose of classification and therefore inconclusive.
CLP: inconclusive
DSD: inconclusive
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