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EC number: 274-778-7 | CAS number: 70693-62-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2001-03-15 - 2001-10-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.5100 - Bacterial Reverse Mutation Test (August 1998)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Pentapotassium bis(peroxymonosulphate) bis(sulphate)
- EC Number:
- 274-778-7
- EC Name:
- Pentapotassium bis(peroxymonosulphate) bis(sulphate)
- Cas Number:
- 70693-62-8
- Molecular formula:
- H3K5O18S4
- IUPAC Name:
- pentapotassium bis((hydroperoxysulfonyl)oxidanide) hydrogen sulfate sulfate
- Test material form:
- solid
Constituent 1
Method
- Target gene:
- not indicated
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535
- Additional strain / cell type characteristics:
- other: histidine missense mutation (hisG46), deficient in a DNA repair system (uvrB), defective lipopolysaccharide coat on the wall (rfa)
- Species / strain / cell type:
- S. typhimurium TA 1537
- Additional strain / cell type characteristics:
- other: histidine frameshift mutation (hisC3076), defective in a DNA repair system and lipopolysaccharide coat
- Species / strain / cell type:
- S. typhimurium TA 98
- Additional strain / cell type characteristics:
- other: histidine frameshift mutation (hisD3052), defective in a DNA repair system and lipopolysaccharide coat
- Species / strain / cell type:
- S. typhimurium TA 100
- Additional strain / cell type characteristics:
- other: histidine missense mutation (hisG46), deficient in a DNA repair system (uvrB), defective lipopolysaccharide coat on the wall (rfa)
- Species / strain / cell type:
- E. coli WP2 uvr A pKM 101
- Additional strain / cell type characteristics:
- other: tryptophan dependent mutant
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix from Aroclor 1254 induced male Sprague-Dawley rats
- Test concentrations with justification for top dose:
- First test (range finding):
5000, 1500, 500, 150, 50, 15 and 5 µg/plate (with and without metabolic activation). Based on the results of the range-finding (cytotoxicity) test, the following concentrations were selected for the main test:
Second (main) test:
5000, 1500, 500, 150 and 50 µg/plate with metabolic activation
500, 150, 50, 15 and 5 µg/plate without metabolic activation - Vehicle / solvent:
- not indicated
Controls
- Untreated negative controls:
- yes
- Remarks:
- water
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: Without metabolic activation: Sodium azide, 9 Aminoacridine, 2 Nitrofluorene, 2 (2 Furyl) 3 (5 nitro 2 furyl)acrylamide With metabolic activation: 2-Aminoanthracene, Benzo[a]pyrene
- Details on test system and experimental conditions:
- METHOD OF APPLICATION:
Aliquots of 0.1 mL of the test dilution (in water), positive or negative control, were placed in glass vessels.
0.5 mL S9 mix or 0.5 mL 0.1 M phosphate buffer (pH 7.4) was added, followed by 0.1 mL of a 10 hour bacterial culture and 2 mL of agar containing histidine (0.5 mM) and tryptophan (0.5 mM) at 45+/-2°C. The mixture was thoroughly shaken and overlaid onto previously prepared petri dishes containing 25 mL minimal agar. Three petri dishes were used for each concentration. All plates were incubated at 37°C for ca. 72 hours.
DURATION
- Preincubation period: In the second test:
Tubes, which contained mixtures of bacteria, buffer or S9 mix and test dilution, were incubated at 37°C for 30 min. with shaking before the addition of the agar overlay.
- Exposure duration: 72 hours
NUMBER OF CELLS EVALUATED: 10 E09 - Evaluation criteria:
- For a test to be considered valid the mean of the solvent/vehicle control revertant colony number for each strain should lie within the historical control range.
The positive control compounds must cause at least a doubling of mean revertant colony number over the negative control.
The mean number of revertant colonies for each treatment group was compared with those obtained for the solvent/vehicle control groups. - Statistics:
- not indicated
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 5000 µg/plate with S9 mix; 500 µg/plate without S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 5000 µg/plate with S9 mix; 500 µg/plate without S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 5000 µg/plate with S9 mix; 500 µg/plate without S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 5000 µg/plate with S9 mix; 500 µg/plate without S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A pKM 101
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 5000 µg/plate with S9 mix; 1500 µg/plate without S9 mix;
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- GENOTOXICITY:
Please refer to tables 1 and 2, which are presented under "Remarks on results including tables and figures"
- without metabolic activation: No increase in the number of revertants/plate observed
- with metabolic activation: No increase in the number of revertants/plate observed
CYTOTOXICITY:
Toxicity was seen in all strains following exposure to KMPS triple salt at 5000 µg/plate in the presence of S9 mix, and at 1500 µg/plate (E. coli) or 500 µg/plate (S. typhimurium) in the absence of S9 mix.
Any other information on results incl. tables
Table 1: Results test 1 (plate-incorporation method)
Plate |
S9 mix |
Revertant colony mean counts |
||||
TA98 |
TA100 |
TA1535 |
TA1537 |
WP2uvr/pKM101 |
||
S9mix, sterility check |
+ |
0 |
0 |
0 |
0 |
0 |
Buffer, sterility check |
- |
0 |
0 |
0 |
0 |
0 |
KMPS triple salt, sterility check |
- |
0 |
0 |
0 |
0 |
0 |
KMPS triple salt, 5000 µg/plate |
+ |
4±3* |
8±7* |
3±4* |
1±1* |
34±9 |
KMPS triple salt, 1500 µg/plate |
+ |
39±4 |
129±7 |
15±2 |
15±2 |
130±12 |
KMPS triple salt, 500 µg/plate |
+ |
40±5 |
133±9 |
17±6 |
20±7 |
124±9 |
KMPS triple salt, 150 µg/plate |
+ |
38±6 |
145±15 |
22±2 |
20±4 |
150±19 |
KMPS triple salt, 50 µg/plate |
+ |
39±5 |
135±14 |
17±5 |
20±6 |
163±22 |
KMPS triple salt, 15 µg/plate |
+ |
45±10 |
140±11 |
20±5 |
22±3 |
141±12 |
KMPS triple salt, 5 µg/plate |
+ |
48±5 |
143±10 |
19±2 |
18±2 |
143±12 |
Water, 0.1 mL/plate |
+ |
45±3 |
148±3 |
21±3 |
22±2 |
162±8 |
KMPS triple salt, 5000 µg/plate |
- |
0±0* |
0±0* |
0±0* |
0±0* |
0±0 |
KMPS triple salt, 1500 µg/plate |
- |
0±0* |
0±0* |
0±0* |
0±0* |
12±9 |
KMPS triple salt, 500 µg/plate |
- |
1±1* |
13±6* |
2±2* |
1±1* |
129±12 |
KMPS triple salt, 150 µg/plate |
- |
37±4 |
121±4 |
13±4 |
14±3 |
117±1 |
KMPS triple salt, 50 µg/plate |
- |
37±4 |
139±8 |
15±4 |
11±3 |
114±3 |
KMPS triple salt, 15 µg/plate |
- |
36±6 |
132±13 |
17±3 |
11±2 |
131±12 |
KMPS triple salt, 5 µg/plate |
- |
37±2 |
115±16 |
20±4 |
14±2 |
125±15 |
Water, (0.1 ml/plate) |
- |
39±5 |
135±8 |
18±3 |
14±2 |
131±10 |
Benzo[a]pyrene, (5 µg/plate) |
+ |
702±77 |
989±24 |
452±56 |
209±13 |
806±72 |
2-Nitrofluorene, (1 µg/plate) |
- |
319±25 |
622±58 |
291±46 |
382±20 |
527±21 |
10-6 dilution of overnight culture, plated on nutrient agar |
- |
104±6 |
106±1 |
130±8 |
110±11 |
169±16 |
* cytotoxicity
Table 2: Results test 2 (with pre‑incubation)
Plate |
S9 mix |
Revertant colony mean counts |
||||
TA98 |
TA100 |
TA1535 |
TA1537 |
WP2uvr/pKM101 |
||
S9mix, sterility check |
+ |
0 |
0 |
0 |
0 |
0 |
Buffer, sterility check |
- |
0 |
0 |
0 |
0 |
0 |
KMPS triple salt, sterility check(5000 µg/plate) |
- |
0 |
0 |
0 |
0 |
0 |
KMPS triple salt, 5000 µg/plate |
+ |
13±4 |
41±5 |
6±2 |
2±1 |
21±2 |
KMPS triple salt, 1500 µg/plate |
+ |
41±6 |
138±6 |
14±1 |
14±3 |
115±25 |
KMPS triple salt, 500 µg/plate |
+ |
42±8 |
134±8 |
19±7 |
14±1 |
138±21 |
KMPS triple salt, 150 µg/plate |
+ |
49±8 |
139±11 |
18±4 |
18±4 |
136±8 |
KMPS triple salt, 50 µg/plate |
+ |
38±7 |
122±14 |
16±4 |
15±2 |
141±5 |
Water, 0.1 mL/plate |
+ |
49±4 |
151±5 |
22±3 |
18±3 |
146±4 |
KMPS triple salt, 500 µg/plate |
- |
12±3 |
73±11 |
4±1 |
2±2 |
8±5 |
KMPS triple salt, 150 µg/plate |
- |
31±4 |
122±11 |
17±5 |
8±1 |
108±6 |
KMPS triple salt, 50 µg/plate |
- |
31±9 |
114±13 |
19±2 |
12±3 |
117±13 |
KMPS triple salt, 15 µg/plate |
- |
33±6 |
131±10 |
18±7 |
9±2 |
121±12 |
KMPS triple salt, 5 µg/plate |
- |
32±5 |
122±15 |
16±5 |
10±1 |
107±10 |
Water, (0.1 ml/plate) |
- |
33±6 |
123±6 |
19±1 |
13±3 |
120±13 |
Benzo[a]pyrene, (5 µg/plate) |
+ |
778±71 |
1006±67 |
251±32 |
386±20 |
1461±396 |
2-Nitrofluorene, (1 µg/plate) |
- |
268±65 |
711±189 |
419±38 |
967±177 |
772±146 |
10-6 dilution of overnight culture, plated on nutrient agar |
- |
116±3 |
176±10 |
131±16 |
120±8 |
180±14 |
Applicant's summary and conclusion
- Conclusions:
- The study and the conclusions which are drawn from it fulfill the quality criteria (validity, reliability, repeatability). Under the test conditions employed, KMPS triple salt showed no evidence of mutagenic activity in this bacterial system in the absence or presence of metabolic activation.
- Executive summary:
Materials and methods
The mutagenic potential of KMPS triple salt was investigated in the Ames test using the histidine auxotroph S. typhimurium strains TA98, TA100, TA1535, TA1537, and a tryptophan dependent mutant of E. coli, WP2uvrA/pKM101, all with and without metabolic activation by rat liver S9 mix from Aroclor 1254 induced male Sprague-Dawley rats. KMPS triple salt was tested at concentrations of 5000, 1500, 500, 150 and 50 µg/plate with metabolic activation and 500, 150, 50, 15 and 5 µg/plate without metabolic activity. The solvent used for the test substance was water.
Results and discussion
No substantial increases in revertant colony numbers over control counts were obtained with any of the tester strains following exposure to KMPS triple salt at any concentration in the presence or absence of S9 mix.
Toxicity was seen in all strains following exposure to KMPS triple salt at 5000 µg/plate in the presence of S9 mix, and at 1500 µg/plate (E. coli) or 500 µg/plate (S. typhimurium) in the absence of S9 mix.
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