Amines, polyethylenepoly-

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

02-03-1989 to 28-04-1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Test is valid and performed under GLP, method is clear and is reproducable. however no analysis was performed on the actual test substance concentration. This makes it only partly usable for risk assessment because the actual bioavailable fraction of the test substance is unknown.

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Qualifier:

according to guideline

Guideline:

other: S.A.L.M. Kooyman: "Parametric Analysis of mortality rates in bio-assays". Water Research 15(1981)107-119.

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

A stock solution of 25 g/L was prepared by dissolving the test
substance in demineralized water containing concentrated HCl.
The presence of the test substance in the test medium caused a
change of the pH, which was outside the range that can be supported
by the test animal. Therefore, the pH was neutralized
both in the stock solution and in the dilution water to a
value between 7.0 and 7.3. The chosen test concentrations were
prepared by dilution of the stock solution.

Test organisms (species):

Poecilia reticulata

Details on test organisms:

The test organism was Poecilia reticulata (guppy); they were
obtained from the lOB (Instituut Onderzoek Bestrijdingsmiddelen), Wageningen, The Netherlands and maintained in the Akzo
Research Laboratories Arnhem, dept. CRL according to Standard
Operation Procedures SOP CRL T9 & 42. The size was approximately 2 cm.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

68.4 mg/L Ca; 35.7 mg/L Mg; 17.7 dH

Test temperature:

21.7-23 C

pH:

7.0-7.7

Dissolved oxygen:

6.7-8.3 mg/L

Nominal and measured concentrations:

nominal: 32, 56, 100, 180, 320, 560 mg/L

Details on test conditions:

The temperature was kept between 21. 7 and 23.0°C, and the
light regime was 15 hours ambient light per day.
The duration of the test was 96 hours.
The test vessels were 2 l glass aquaria, containing 1 l of
test medium.
The loading in the test vessels was approximately 0.6 g biomass/L

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 75-131 mg/L

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

180 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality occurred at 32 mg/l, and 100% mortality occurred at 180 mg/l. The
LC50-96 is 100 mg/l. The 95% confidence limits are 75 and 131 mg/l.
The quality criteria of this test have been fulfilled: the mortality
in the control was <=10%, the oxygen concentration was >60% of
the air-saturation value throughout the test, and the concentration
of HEPA in the test medium remained constant.

Reported statistics and error estimates:

The LC50 was determined with an LC50 program of Griffioen
(R1ZA), based on a model of Kooyman (1981).

Sublethal observations / clinical signs:

Nominal concentration mg/L	Number of surviving animals
	0h	24h	48h	72h	96h
Control 1	10	10	10	10	10
Control 2	10	10	10	10	10
Control 3	10	10	10	10	10
32	10	10	10	10	10
56	10	10	10	8	8
100	10	10	9	8	6*
180	10	9	8	2	0
320	10	10	6	1	0
560	10	10	2	0	0

* number not healthy

Validity criteria fulfilled:

yes

Conclusions:

The LC50 was determined to be 100 mg/L nominal of HEPA. The study meets all criteria however no analysis was done so the use for risk assessment purposes is restricted to only nominal concentrations

Executive summary:

The fraction HEPA (Higher ethylene polyamines) was tested in an acute

toxicity test with guppy (Poecilia reticulata). The test was performed according to the EEC method, part c.:

Methods for the determination of ecotoxicity, C.l. Acute toxicity to fish.

The test was performed as a semi-static test, in which the solution was renewed after 48 hours. During this time HEPA is

not (bio)degradable. No mortality was observed up to 32 mg/l. The LC50-96 h was 100 mg/l,

with 95% confidence limits of 75 and 131 mg/l. no analysis was performed so all concentrations are nominal, this makes a restrictionfor the use for risk assessment uses

Description of key information

 EU method C.1, Poecilia reticulate: LC50(96 h) of 100 mg/L (nominal) based on mortality. 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

The acute toxicity of the substance to fish was tested in a guideline study following EU Method C.1. The study was conducted according the GLP specifications. Fish of the species Poecilia reticulata were exposed to nominal substance concentrations of 32, 56, 100, 180, 320, 560 mg/L. The duration of the semi-static test was 96 hours. At test termination a LC50 (96 h) of 100 mg/L (nominal) was determined.