Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-375-3 | CAS number: 120-18-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
Valid experimental data were available to assess the genetic toxicity in vitro:
1.) Gene mutation in bacteria
Naphthalenesulfonic acids (purity 86%) were not mutagenic in a Ames test with and without metabolic activation according to OECD test guideline 471 (tested up to 5000 μg/plate in Salmonella typhimurium TA1535, TA 1537, TA 98 and TA 100; metabolic activation: S-9 mix from Aroclor 1254 induced rat liver; BASF AG 1989). The test was performed as independent standard plate test and preincubation test. Cytotoxicity (reduction of the background lawn) was not observed.
2.) Cytogenicity in mammalian cells
Naphthalenesulfonic acids (purity unknown) were tested for their potential to induce chromosomal aberrations in Chinese hamster lung cells (V79) in a GLP conform study according to OECD test guideline 473 (BAUA 1993). The test substance did not induce chromosomal aberrations with or without S-9 mix as exogenous metabolic activation system; the trials were performed up to toxic doses of 2500 µg/mL.
3.) Mammalian cell gene mutation assay
Naphthalinsulfonsaeureschmelze (NSS, purity 80 area-%) was tested in an in vitro GLP guideline study (BASF SE, 2010) for its potential to induce gene mutations at the HPRT locus in V79 cells with and without metabolic activation. Cytotoxicity (<= 50 % viability compared to vehicle control) was observed at >= 1875 µg/ml (without metabolic activation, 24 h treatment) or >= 468.8 µg/ml (with metabolic activation, 4 h treatment), respectively. Neither a relevant and reproducible increase in mutant colony numbers/10e6 cells nor a significant dose dependent trend of the mutation frequency was observed up to the maximum concentrations (2500 µg/ml without metabolic activation; 468.8 µg/ml with metabolic activation). Therefore, under the experimental conditions reported, NSS was considered to be non-mutagenic.
In vivo:
No data available.
Read across justification:
The registration item contains ca. 78.89% of naphthalene-2-sulphonic acid (CAS # 120-18-3) and ca. 6.5 % of naphthalene-1-sulphonic acid (CAS # 85-47-2). These two substances have the same molecular weight and are structurally almost identical. Therefore naphthalene-2-sulphonic acid and naphthalene-1-sulphonic acid and their respective salts are suitable for read across in order to fulfill the data requirements.
Short description of key information:
Gene mutation in bacteria
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100, with and without metabolic activation (Ames test): negative (OECD 471; BASF AG 1989)
Cytogenicity in mammalian cells
CHL V79 cells (chromosomal aberration test), without metabolic activation: negative (GLP, OECD 473; BAUA 1993)
In vitro mammalian cell gene mutation assay
HPRT assay, CHL V79 cells, with and without metabolic activation: negative (GLP, OECD 476, BASF SE 2010)
Mutagenicity in vivo
No data available
Endpoint Conclusion: No adverse effect observed (negative)
Justification for classification or non-classification
The test substance was not genotoxic in in vitro experiments using mammalian and bacterial cells. For naphthalenesulfonic acids, there is therefore no need for classification for mutagenic effects.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.