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EC number: 248-145-0 | CAS number: 26966-75-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Pre-GLP study, fully reported, essentially to guideline. No E coli or TA102 strains used.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Principles of method if other than guideline:
- Duplicate plates used. Four test concentrations, up to limit.
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3(or 4)-methylbenzene-1,2-diamine
- EC Number:
- 248-145-0
- EC Name:
- 3(or 4)-methylbenzene-1,2-diamine
- Cas Number:
- 26966-75-6
- Molecular formula:
- C7H10N2
- IUPAC Name:
- 3(or 4)-methylbenzene-1,2-diamine
- Details on test material:
- 3,4-TDA, CAS 496-72-0. 97% purity
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- Rat S-9, induced with Aroclor 1254.
- Test concentrations with justification for top dose:
- 1000, 1710, 2924, 5000,
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other:
- Remarks:
- Positive control S9 varied according to tester strain and use of S9.
- Details on test system and experimental conditions:
- Plate incopporation method. Duplicate plates.
- Statistics:
- None
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- 3.4-TDA was marginally active in strains TA98 and TA1538, although no dose-response relationship was evident.
3,4-TDA was more toxic to cells in the absence of S9, and was particularly toxic to TA98 and TA 1538 strains. All doses tested were toxic.
Any other information on results incl. tables
Bacterial Mutagenicity of 3,4-TDA
Chemical |
Amount per plate (ug) |
S-9b |
Revertants per platea |
||||
TA98 |
TA100 |
TA1535 |
TA1537 |
TA1538 |
|||
Negative control |
- |
- |
13 |
54 |
54 |
6 |
8 |
Negative control |
- |
+ |
17 |
55 |
52 |
7 |
11 |
Positive controlc |
- |
- |
558 |
237 |
78 |
122 |
1209 |
Positive controld |
- |
+ |
1178 |
242 |
0 |
379 |
1156 |
3,4-TDA |
1000 |
- |
11 |
60 |
46 |
4 |
6 |
3,4-TDA |
1710 |
- |
20 |
66 |
51 |
8 |
6 |
3,4-TDA |
2924 |
- |
7 |
42 |
15 |
7 |
6 |
3,4-TDA |
5000 |
- |
4 |
14 |
1 |
4 |
4 |
3,4-TDA |
1000 |
+ |
48 |
68 |
70 |
12 |
56 |
3,4-TDA |
1710 |
+ |
54 |
64 |
58 |
13 |
56 |
3,4-TDA |
2924 |
+ |
59 |
62 |
62 |
9 |
48 |
3,4-TDA |
5000 |
+ |
48 |
55 |
58 |
5 |
26 |
a Average of two plates
b Aroclor 1254 induced Fisher 344 rat liver.
c The following positive controls were used without activating medium: dinitrotoluene (500 ug, TA1535 and TA100), quinacrine mustard (10ug, TA1537) and nitrofluorene (100 ug, TA1538 and TA98)
d The following positive controls were used with activating medium, anthramine (100 ug, TA1535 and TA100), aminoquinoline (100 ug, TA1537) and acetamidofluorene (500 ug, TA1538 and TA98)
Toxicity of TDA Isomers for S. Typhimurium
Chemical |
Amount per plate (ug) |
S-9b |
Relative Survivala |
||||
TA98 |
TA100 |
TA1535 |
TA1537 |
TA1538 |
|||
3,4-TDA |
1000 |
- |
0.107 |
0.463 |
0.803 |
0.794 |
0.341 |
3,4-TDA |
1710 |
- |
0.094 |
0.473 |
0.767 |
0.811 |
0.302 |
3,4-TDA |
2924 |
- |
0.068 |
0.471 |
0.598 |
0.712 |
0.185 |
3,4-TDA |
5000 |
- |
0.018 |
0.411 |
0.407 |
0.326 |
0.042 |
3,4-TDA |
1000 |
+ |
0.224 |
0.471 |
0.901 |
0.872 |
0.320 |
3,4-TDA |
1710 |
+ |
0.247 |
0.501 |
0.904 |
0.868 |
0.313 |
3,4-TDA |
2924 |
+ |
0.240 |
0.422 |
0.816 |
0.861 |
0.309 |
3,4-TDA |
5000 |
+ |
0.194 |
0.385 |
0.773 |
0.875 |
0.309 |
a The amount of chemical indicated, + or - rat S-9, was added to a tube of top agar containing 0.1 ml of 1 10-6 dilution of the relevant tester strain. The mixture was poured onto a nutrient agar plate, was allowed to harden, and the plates were incubated at 37°C for 24 hr.
Relative Survival = Number of colonies on plate + chemical
................................Number of colonies on control plate
b Aroclor 1254 induced Fisher 344 rat liver.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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