Diallyldimethylammonium chloride

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented report of a guideline study conducted to GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Diallyldimethylammonium chloride

EC Number:

230-993-8

EC Name:

Diallyldimethylammonium chloride

Cas Number:

7398-69-8

Molecular formula:

C8H16N.Cl

IUPAC Name:

diallyldimethylammonium chloride

Details on test material:

- Name of test material (as cited in study report): Dimethyldiallylammoniumchlorid ca 60%
- Physical state: liquid
- Analytical purity: no data given
- Composition of test material, percentage of components: Dimethyldiallylammoniumchlorid ca 60% in water
- Lot/batch No.: 76334
- Expiration date of the lot/batch: not given
- Stability under test conditions: stable
- Storage condition of test material: no special conditions

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

yes

Details on test solutions:

water

Test organisms

Test organisms (species):

other: activated sludge from laboratory wastewater plants treating municipal and synthetic sewage.

Details on inoculum:

Preparation of the inoculum: The inoculum was washed, brought to a concentration of 5 g/l and aerated for 24 hours. 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/l dry substance in the test.

Study design

Test type:

static

Water media type:

brackish water

Limit test:

yes

Total exposure duration:

30 min

Post exposure observation period:

none

Test conditions

Hardness:

no data

Test temperature:

20+/-2°C

pH:

7.5

Dissolved oxygen:

During aeration >2.5 mg/L; immediately before measurement > 6.5 mg/l

Salinity:

no data

Nominal and measured concentrations:

nominal 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: Erlenmeyer flask
- Size: nominal volume of 250 ml
- Aeration: yes
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 1g/L dry substance
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: no data given
- Culture medium different from test medium: no
EFFECT PARAMETERS MEASURED: oxygen concentration at beginning and after 30 minutes.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: maximum concentration

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Duration:

30 min

Dose descriptor:

EC0

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: Inhibition of oxygen consumption

Details on results:

No abnormal observations or inconsistencies.

Results with reference substance (positive control):

- Results with reference substance valid?: yes
- Relevant effect levels: EC50 of 3,5-dichlorophenol in the range of 5-30 mg/L (approx. 9 mg/l)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

No significant inhibition of respiration was measured up to the highest tested concentration of 1000 mg/L (nominal) Dimetyldiallylammoniumchlorid ca 60% (600 mg/l DADMAC)