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EC number: 203-309-0 | CAS number: 105-56-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Ethyl cyanoacetate
- EC Number:
- 203-309-0
- EC Name:
- Ethyl cyanoacetate
- Cas Number:
- 105-56-6
- Molecular formula:
- C5H7NO2
- IUPAC Name:
- ethyl 2-cyanoacetate
- Details on test material:
- Batch No. Tank 434
ID No. 3633/81 410
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 6 to 8 weeks
- Fasting period before study: ca 16 hours
- Housing: conventionel, by gender separated, max. 5 animals
- Diet: 3 hours p.a. ad libitum
- Water: ad libitum
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 ± 3°C
- Humidity (%): 30 - 70 %
- Air changes: 15 times/ h
- Photoperiod: 12 h dark/ 12 h light
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- not specified
- Details on oral exposure:
- application volume: 1.89 cm³/kg body weight
- Rationale for the selection of the starting dose: - Doses:
- 2000 mg ethyl cyanoacetate pro kg body weight
- No. of animals per sex per dose:
- 5 female animals per dose
5 male animals per dose - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: 1/2, 1, 2, 3, 4, 5, 6 hours p.a., after this daily
- Frequency of weighing: day 0, 7, 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ changes
Results and discussion
- Preliminary study:
- 2000 mg ethyl cyanoacetate pro kg body weight were administered to 2 male and 2 female rats as a preliminary study. The results were recognized in the test outcome.
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No mortality was observed.
- Clinical signs:
- other: Observations were performed 0.5, 1, 2, 3, 4, 5, 6, hours after administration (p.a.) of the test substance and then at least once a day for a total of 2 weeks. 2 - 6 hours p.a. all animals indicated symptoms of poisoning. All animals showed changes in the
- Gross pathology:
- All animals were killed by CO2 14 days p.a. and subjected to a necropsy including a gross pathological examination.
The pathological examination showed no signs for macroscopic changes of the organs conditional on the test substance.
Applicant's summary and conclusion
- Interpretation of results:
- practically nontoxic
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The LD50 of ethyl cyanoacetate is estimated to be higher than 2000 mg/kg body weight in rats for a single dose.
The pathological examination showed no signs for macroscopic changes of the organs conditional on the test substance.
Therefore the test substance ethyl cyanoacetate is practically nontoxic according to the EC-guideline 93/21. - Executive summary:
Aim of the study
It was the aim of the study to investigate acute toxic effects of the test substance after a single oral administration.
Methods
The OECD-Guideline 401, "Acute Oral Toxicity" OECD 1981, updated Guideline was adopted in Feb. 1987, was applied.
Administration
Ethyl cyanoacetate was administered once oral by stomach intubation to Bor: WISW (SPF Cpb) rats. The dosing was performed sequentially to 5 male and 5 female animals, each using a dose of 2000 mg per kg body weight.
The dose volume was 1.89 cm³ per kg body weight for all animals.
Investigations
Body weight: before administration, 7 and 14 days p.a.
Clinical observations were performed 0.5, 1, 2, 3, 4, 5, 6, hours p.a. of the test substance and then at least once a day for a total of 2 weeks.
Necropsy: all animals were sacrificed and necropsied 14 days p.a.
Results
Mortality
No mortality was observed.
Body weights
Males and females: Body weight and body weight gain of all animals were inconspicuous.
Clinical observations
2 - 6 hours p.a. all animals indicated symptoms of poisoning. All animals showed changes in the fur. Some animals showed changes in gait, ventral and lateral position, sedation or ataxia, decreased motility, tremor, vocalization, stagger, increased respiratory frequency, Straub phenomenon and disturbance of equilibrium.
Necropsy findings
The pathological examination showed no signs for macroscopic changes of the organs conditional on the test substance.
Sex differences
The response to the test substance at 2000 mg/kg body weight did not indicate a sex difference.
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