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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Endpoint:
fish embryo acute toxicity (FET)
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 236 (Fish embryo acute toxicity (FET) test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium valproate
EC Number:
213-961-8
EC Name:
Sodium valproate
Cas Number:
1069-66-5
Molecular formula:
C8H16O2.Na
IUPAC Name:
sodium 2-propylpentanoate

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Remarks:
treated mains water

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:
semi-static
Water media type:
other: treated mains water
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
63 mg/L as CaCO3
Test temperature:
26°C +/- 1°C
pH:
7.02 to 7.33
Dissolved oxygen:
92 mg O2/L
8.14 % (air saturation value)
Conductivity:
236 µS
Nominal and measured concentrations:
10, 18, 32, 56 and 100 mg/L (nominal concentrations)
Reference substance (positive control):
yes
Remarks:
3,4-dichloroaniline

Results and discussion

Effect concentrations
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
66 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
The 95% confidence limits for 96h LC50 are: 54-80 mg/L
Results with reference substance (positive control):
For the reference substance, 3,4-dichloroaniline, the observed mortality was 100% which was in excess of the minimum 30% validity criteria.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, the 96-hour LC50 of sodium valproate was considered to be 66 mg/L.