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EC number: 212-298-1 | CAS number: 778-94-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 Feb – 22 Mar 2019
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- GLP compliance:
- yes
- Remarks:
- The test was performed in compliance with the requirements of the Principles of Good Laboratory Practices (GB/T 22278-2008).
Test material
- Reference substance name:
- 2-nitro-4-(trifluoromethyl)benzonitrile
- EC Number:
- 212-298-1
- EC Name:
- 2-nitro-4-(trifluoromethyl)benzonitrile
- Cas Number:
- 778-94-9
- Molecular formula:
- C8H3F3N2O2
- IUPAC Name:
- 2-nitro-4-(trifluoromethyl)benzonitrile
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Control, 1.0, 2.0, 4.0, 8.0 and 16.0 mg/L
- Sampling method: 10 mL water samples were taken at 0, 24 and 48h from the blank control group and each treatment group
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 0.0101 g test substance was weighed, dissolved with Elendt M7, subjected to ultrasound for 20 minutes and diluted to 100 mL, yielding 100 mg/L test substance stock solution.
- Controls: negative control (Elendt M7 medium)
- Test concentrations separation factor: 2
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: Batch number: D20190320D
- Age at study initiation (mean and range, SD): younger than 24 hours
- Stage and instar at study initiation: The Daphnia used in the toxicity test were healthy parthenogenetic female Daphnia, not first born.
- Source: Nanjing Institute of Environmental Sciences of the Ministry of Environmental Protection obtained in 2018
- Feeding during test: no
ACCLIMATION
- Acclimation period: Held in test facility since 2018
- Acclimation conditions (same as test or not): same
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20.2 - 20.7 °C.
- pH:
- 7.34 - 8.03
- Dissolved oxygen:
- 6.46 - 8.60 mg/L
- Nominal and measured concentrations:
- Nominal concentrations: 0.0 (Control), 1.0, 2.0, 4.0, 8.0 and 16.0 mg/L
Mean measured concentrations: < LOD/LOQ, 0.973, 1.88, 3.80, 7.50 and 14.9 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL beakers (100 mL, Tianbo Instruments Factory, with breathable lid)
- Type (delete if not applicable): open
- Volume of solution: 50 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M7 medium was used as test medium
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH, dissolved oxygen, and temperature were measured at 0 and 48 hours.
OTHER TEST CONDITIONS
- Photoperiod: Light/dark ratio 16:8 h
- Light intensity: 1430 lux to 1476 lux
EFFECT PARAMETERS MEASURED: Immobilisation of daphnids was observed daily (0, 24, 48 h).
RANGE-FINDING STUDY
- Test concentrations: Control, 1.00 mg/L, 10.0 mg/L, 100 mg/L
- Results used to determine the conditions for the definitive study: 0% inhibition rate at 1 mg/L; 100% inhibition rate at 10 and 100 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate was used as reference compound and tested at least once a year to verify that the test conditions satisfied the test requirements and no significant changes had occurred.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.55 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: Activity of young Daphnia was normal in the blank control group and in the 1.00 mg/L, 2.00 mg/L, and 4.00 mg/L test groups and no significant inhibition symptoms were apparent.
- Immobilisation: In the 8.00 mg/L and 16.0 mg/L test groups, deaths and immobilisation occurred in Daphnia. - Results with reference substance (positive control):
- EC50 (48 h) = 1.23 mg/L (nominal) obtained in test from test period of 16 to 28 Feb 2019.
Any other information on results incl. tables
Table 1: Measurement Results for Stability of the Test Substance in Test Medium
Prepared concentration (mg/L) |
Measured concentration (mg/L) |
Mean actual measured concentration/0 h actual measured concentration |
|||
0 h |
24 h |
48 h |
Mean value |
||
0.00 |
ND1 |
ND |
ND |
ND |
- |
1.00 |
0.989 |
0.980 |
0.951 |
0.973 |
98.4% |
2.00 |
1.95 |
1.83 |
1.85 |
1.88 |
96.2% |
4.00 |
3.94 |
3.76 |
3.69 |
3.80 |
96.4% |
8.00 |
7.55 |
7.48 |
7.46 |
7.50 |
99.3% |
16.0 |
15.2 |
14.9 |
14.7 |
14.9 |
98.2% |
1: Concentration not detected.
Table 2: Observation Results from Actual test
Prepared concentration (mg/L) |
Observation results |
|
24 h |
48 h |
|
Blank control |
5 NB |
5 NB |
5 NB |
5 NB |
|
5 NB |
5 NB |
|
5 NB |
5 NB |
|
1.0 |
5 NB |
5 NB |
5 NB |
5 NB |
|
5 NB |
5 NB |
|
5 NB |
5 NB |
|
2.0 |
5 NB |
5 NB |
5 NB |
5 NB |
|
5 NB |
5 NB |
|
5 NB |
5 NB |
|
4.0 |
5 NB |
5 NB |
5 NB |
5 NB |
|
5 NB |
5 NB |
|
5 NB |
5 NB |
|
8.0 |
5 NB |
1 D 4 NB |
5 NB |
2 D 3 NB |
|
5 NB |
5 D |
|
5 NB |
5 D |
|
16.0 |
5 D |
5 D |
5 D |
5 D |
|
5 D |
5 D |
|
5 D |
5 D |
NB: normal activity.
D: dead.
Table 3: Validity criteria for OECD 202 (2004)
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
In the control 0% of the daphnids were immobilized. |
yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
Dissolved oxygen in the blank control group and the test treatment group was greater than 3 mg/L (minimum value: 6.46, maximum value: 8.60). |
yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- See Table 3 in "Any other information on results incl. tables".
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