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Diss Factsheets
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EC number: 254-274-3 | CAS number: 39072-70-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- N,N''-hexane-1,6-diylbis[N'-benzylurea]
- EC Number:
- 254-274-3
- EC Name:
- N,N''-hexane-1,6-diylbis[N'-benzylurea]
- Cas Number:
- 39072-70-3
- Molecular formula:
- C22H30N4O2
- IUPAC Name:
- N',N'''-hexane-1,6-diylbis(1-benzylurea)
- Details on test material:
- Description White powder (determined at NOTOX)
Batch 259-635
Purity > 90%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Frequency at t=0 h and t=48 h
Volume 2 ml from the approximate centre of the test vessels
Storage Samples were stored in a freezer until analysis.
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnids originated from a healthy stock, 2nd to 5th brood, showing no signs of stress such as mortality >20%, presence of males, ephippia or discoloured animals and there was no delay in the production of the first brood.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 180 mg/l as CaCO3
- Test temperature:
- 19.7 - 20.8°C
- pH:
- 7.8
- Dissolved oxygen:
- 8.9 - 9.8
- Nominal and measured concentrations:
- Analysis of the samples taken from the filtrates prepared at 1.0 and 100 mg/l showed that measured concentrations remained fairly stable over the test period. Analyses of the filtrate prepared at 1.0 mg/l showed measured concentrations of 11.0 and 12.1 µg/l at t=0 and 48 hours. Analyses of the filtrate prepared at 100 mg/l showed measured concentrations of 17.1 and 19.6 µg/l at t=0 and 48 hours, respectively. The average measured concentrations were calculated to correspond to 12 and 18 µg/l, for the filtrates prepared at 1.0 and 100 mg/l, respectively.
- Details on test conditions:
- Test vessels 100 ml, all-glass
Medium Adjusted ISO medium
Number of daphnids 20 per concentration
Loading 5 per vessel containing 80 ml of test solution
Light 16 hours photoperiod daily
Feeding No feeding
Aeration No aeration of the test solutions.
Introduction of daphnids Within 1 hour after preparation of the test solutions.
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- The 48h-EC50 exceeded 18 µg/l, being the average measured concentration in a 0.45 µm filtered solution prepared at a loading rate of 100 mg/l.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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