Registration Dossier
Registration Dossier
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EC number: 221-882-5 | CAS number: 3268-49-3
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Version / remarks:
- 1997
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: sampling at 0, 9, 14, 19 and 21 days
- Vehicle:
- not specified
- Test organisms (species):
- Daphnia magna
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Test temperature:
- 20 ± 1 °C
- Nominal and measured concentrations:
- Nominal test substance concentration 0.125, 0.250, 0.500, 1.00, 2.00 mg/L
- Details on test conditions:
- TEST SYSTEM
- Fill volume: 1.8 L
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
OTHER TEST CONDITIONS
- Photoperiod:16 h light, 8 h darkness
- Light intensity: 1200 lux
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Reference substance (positive control):
- not specified
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- 1.09 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% confidence limit: 0.861 to 153 mg/L
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 1.51 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.315 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
Reference
Description of key information
NOEC (21 d): 0.315 mg/L (measured, OECD 211)
Key value for chemical safety assessment
Additional information
The chonic toxicity of the substance to Daphnia magna was investigated in a study following OECD 211. Daphnia magna were exposed for 21 d to nominal substance concentrations of 0.125, 0.250, 0.500, 1.00, 2.00 mg/L in a flow-through system. An analytical dose verification by HPLC was performed. Based on the reproduction rate a NOEC (21 d) of 0.315 mg/L (measured) was determined.
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