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EC number: 227-824-5 | CAS number: 5994-61-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water and sediment: simulation tests
Administrative data
- Endpoint:
- biodegradation in water: sewage treatment simulation testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- Not reported
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, acceptable for assessment.
- Justification for data waiving:
- other:
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Biodegradation potential of the environmental isolations were conducted for degrading microorganisms using samples obtained from soils, sludges and waters. Enrichments for microorganisms capable of degradation were performed using sequencing batch reactors (SBRs), immobilized bacteria columns and anaerobic incubations.
- GLP compliance:
- no
Test material
- Reference substance name:
- N-(carboxymethyl)-N-(phosphonomethyl)glycine
- EC Number:
- 227-824-5
- EC Name:
- N-(carboxymethyl)-N-(phosphonomethyl)glycine
- Cas Number:
- 5994-61-6
- Molecular formula:
- C5H10NO7P
- IUPAC Name:
- 2-[(carboxymethyl)(phosphonomethyl)amino]acetic acid
- Details on test material:
- - Name of test material (as cited in study report): N-phosphonomethyliminodiacetic acid
- Substance type: Pure active substance
- Analytical purity: 97.5-98.0 %
Radiolabeled material 1
- Radiochemical purity (if radiolabelling): 14C-Carboxymethyl labeled GI
- Specific activity (if radiolabelling): 19.0 mCi/mM
- Radiochemical impurity: Iminodiacetic acid (IDA)
- Purity: > 99 %
Radiolabeled material 2
- Radiochemical purity (if radiolabelling): 14CPhosphonomethyl-Iabeled GI
- Specific activity (if radiolabelling): 8.27 mCi/mM
- Radiochemical impurity: Iminodiacetic acid (IDA)
- Purity: > 96 %
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable - Radiolabelling:
- yes
Study design
- Oxygen conditions:
- aerobic/anaerobic
- Inoculum or test system:
- mixture of sewage, soil and natural water
- Details on source and properties of surface water:
- - Details on collection (e.g. location, sampling depth, contamination history, procedure): Sediment plus overlying water from the Cape Fear River
- Details on source and properties of sediment:
- - Details on collection (e.g. location, sampling depth, contamination history, procedure):
Five samples were obtained in or around the Luling site: Sediments from the decant and polishing ponds, soil adjacent to a processing unit, sludge from the aerobic digester and sediment plus overlying water from the Mississippi River.
Four environments were sampled in or around the Fayetteville site: sludges from a SBR and second stage digester, soil surrounding a processing unit, and sediment plus overlying water from the Cape Fear River. - Details on inoculum:
- Composition of the Sequencing Batch Reactors:
- 10 g polishing pond sediment, 5 g Mississippi River sediment, 5 g processing unit soil, 240 mL decant pond sludge and 840 mL digester sludge - Duration of test (contact time):
- 25 d
Initial test substance concentration
- Initial conc.:
- 1.6 g/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- test mat. analysis
- other: Test material degrading activity
- Details on study design:
- Please refer " Any other information on materials and methods incl.table" section
Reference substance
- Reference substance:
- not specified
Results and discussion
- Test performance:
- No data
% Degradationopen allclose all
- % Degr.:
- > 99
- Parameter:
- test mat. analysis
- Sampling time:
- 5 d
- Remarks on result:
- other: In bacterial isolates at 1000 mg/L
- % Degr.:
- > 99
- Parameter:
- other: Degrading activity
- Sampling time:
- 10 h
- Remarks on result:
- other: In immobilized bacteria column (influent concentration of 500 mg/L)
- Transformation products:
- yes
Identity of transformation products
- No.:
- #1
Reference
- Reference substance name:
- Unnamed
- IUPAC name:
- AMPA
- Identifier:
- common name
- Identity:
- AMPA
- Details on transformation products:
- No data
- Evaporation of parent compound:
- not measured
- Volatile metabolites:
- not measured
- Residues:
- not measured
- Details on results:
- TEST CONDITIONS
- Aerobicity (or anaerobicity), moisture, temperature and other experimental conditions maintained throughout the study: Yes - Results with reference substance:
- None
Any other information on results incl. tables
Glyphosate intermediate degrading activity (GI-DA) was established in a SBR. The cycle time required to degrade up to 1.6 g/L glyphosate intermediate (GI) was reduced from 21 to less than 3 d.
Table 1. GI biodegradation by bacterial isolates and SBR sludges in GI minimal media
Inoculum Isolate # |
% GI removal |
1 |
4.7 |
2 |
99.9 |
3 |
1.8 |
4 |
0.0 |
5 |
99.9 |
6 |
0.0 |
7 |
0.0 |
8 |
99.9 |
Mixed liquor- SBR A |
18.2 |
Mixed liquor- SBR B |
31.9 |
Applicant's summary and conclusion
Validity criteria
- Validity criteria fulfilled:
- no
- Conclusions:
- Under the test conditions, the test material is readily biodegradable
- Executive summary:
A study was conducted to assess the biodegradation potential of the test material for degrading microorganisms using samples obtained from soils, sludges and waters.
Enrichments for microorganisms capable of degradation were performed using sequencing batch reactors (SBRs) immobilized bacteria columns, and anaerobic incubations. A microbial population was established which was capable of degrading test material. Test material degrading activity (GI-DA) was determined by HPLC analysis and confirmed by radiolabeled studies.
The cycle time required to degrade up to 1.6 g/L test material in SBRs was reduced from 21 to <3 d. Test material removal was >99% at an influent concentration of 500 mg/L and a hydraulic retention time of <10 h. In shake flask assays, microbial isolates removed 1000 mg/L test material in less than 5 d demonstrating the biodegradation of test material.
Under the test conditions, the test material is readily biodegradable.
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