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EC number: 222-823-6 | CAS number: 3622-84-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 38 mg/l
- Sampling method:Representative water samples of exposure medium, control and solvent control was collected during the conduct of the experiment at
0 and 24h and sent to the department of analytical chemistry for the verification of concentration and stability.
- Sample storage conditions before analysis: no data - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the final solution was warmed between 50-60°c and kept on a magnetic stirrer for 30 minutes to obtain clear solution. Then it was allowed to cool at room temperature and the same was transferred to the exposure medium.
- Eluate:
- Differential loading:
- Controls: control group (without test substance) and solvent control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone as organic solvent
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): the solvent used in the exposure medium did not exceed 0.1 ml/l
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Brachydanio Rerio
- Strain:
- Source: supplied from a commercial fish farm.
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 3 +- 0.5 cm
- Weight at study initiation (mean and range, SD):
- Method of breeding: /
- Feeding during test
- Food type: commercially available aquarium fish feed
- Amount:
- Frequency: daily
ACCLIMATION
- Acclimation period:7 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: commercially available aquarium fish feed
- Feeding frequency: feeding was stopped 24h prior to the commencement of the experiment
- Health during acclimation (any mortality observed): healthy
QUARANTINE (wild caught)
no wild caught
- Duration:
- Health/mortality: - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- The fish was quarantined for a min. of at least 12d in the laboratory before they are used for testing and the fish was fed with
commercially available aquarium fish feed, daily.
Body weight was recorded and length measured in randomly selected ten fish prior to the commencement of the acclimatization.
The fish was acclimatized 7 days prior to the experiment in the test room and the feeding was stopped 24h prior to the commencement of the
experiment. - Hardness:
- control: 200 CaCO3 mg/l
Solvent control (acetone not exceeding 0.1 ml/l): 200 CaCO3 mg/l
38 mg/l: 20/04/2006: 184 mg/l CaCO3 -- 24/04/2006: 194 mg/l CaCO3. - Test temperature:
- control: ca. 22.5°c
Solvent control (acetone not exceeding 0.1 ml/l): ca. 22.5°c
38 mg/l: ca. 22.5 - 25.4 °c - pH:
- control: 8.21-8.26
Solvent control (acetone not exceeding 0.1 ml/l): 8.20 - 8.28
38 mg/l: 8.20 - 8.38 - Dissolved oxygen:
- control: 7.07 - 7.22 mg/l
Solvent control (acetone not exceeding 0.1 ml/l): 7.17 - 7.31 mg/l
38 mg/l: 7.19 - 7.28 mg/l - Salinity:
- NO DATA
- Nominal and measured concentrations:
- 38, 45.6, 54.7, 65.7 and 78.8 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:glass aquaria (10l)
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: 10l
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):renewed daily
- No. of organisms per vessel:10
- No. of vessels per concentration (replicates):5
- No. of vessels per control (replicates):1
- No. of vessels per vehicle control (replicates):1
- Biomass loading rate:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:1120-1158µS
- Culture medium different from test medium:
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Adjustment of pH:8.20-8.38
- Photoperiod:
- Light intensity:12h light, 12h darkness
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 38 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 36.7 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: no signs of toxicity or behavioural abnormalities were observed in fish exposed to control and 38 mg/l BBSA.
- Observations on body length and weight:
- Other biological observations:
- Mortality of control: no mortality was observed in control group, solvent control group and fish exposed to 38 mg/l
- Other adverse effects control:
- Abnormal responses:
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:/
- Effect concentrations exceeding solubility of substance in test medium:/ - Reported statistics and error estimates:
- NO DATA
- Validity criteria fulfilled:
- yes
- Conclusions:
- Short-term toxicity to fish was assessed in a 96h study. The measured LC 50 > 38 mg/L (nominal), the highest concentration tested.
Reference
Description of key information
The acute fish toxicity of BBSA was determined in an OECD 203 test. The measured LC50 was 36.7 mg/l.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 36.7 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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